Developmental Forms of Vaccinia Virus

Gaylord, William H.; Melnick, Joseph L.

doi:10.1126/science.117.3027.10

Cited by 14 publications

(2 citation statements)

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“…Vaccinia-infected chorioallantois has been investigated by Bang (5) who suggested a relationship between mitochondria and virus, and by Wyckoff (31) who pointed out certain structures of low electron density which might have a role in virus development. Using similar material, we have called attention to the perinuclear position of early vaccinia virus development and to a matrix enveloping the earliest forms (13,14). Molluscum contagiosum virus has been identified in thin sections of the human lesion in two laboratories (4,9).…”

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confidence: 99%

Intracellular Forms of Pox Viruses as Shown by the Electron Microscope (Vaccinia, Ectromelia, Molluscum Contagiosum)

Gaylord

Melnick

1953

Journal of Experimental Medicine

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103

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The intracellular development of three pox viruses has been studied with the electron microscope using thin sections of infected tissue. Cells infected with vaccinia, ectromelia, and molluscum contagiosum viruses all form developmental bodies preliminary to the production of mature virus. Developmental bodies, believed to be virus precursors, are round to oval, slightly larger than mature virus particles, less dense to electrons, and have a more varied morphology. It is suggested as a working hypothesis that the process of maturation of a virus particle takes place as follows. In the earliest form the developmental bodies appear as hollow spheres, imbedded in a very dense cytoplasmic mass constituting an inclusion body, or in a less dense matrix near the nucleus in cells without typical inclusion bodies. The spheres become filled with a homogeneous material of low electron density. A small, dense granule appears in each developmental body and grows in size at the expense of the low density material. Following growth of the granule, particles are found with the dimensions of mature virus and having complex internal structure resembling bars or dumbells. Mature virus is ovoid and very dense to electrons. An "empty" interior may be found within its thick walls.

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Intracellular Forms of Pox Viruses as Shown by the Electron Microscope (Vaccinia, Ectromelia, Molluscum Contagiosum)

Gaylord

Melnick

1953

Journal of Experimental Medicine

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103

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“…VACV regulatory protein E6 is known to be essential for core protein association. By condensing into the dense brick-shaped mature virions (MVs), the IVs then maturate into intracellular mature virus (IMV), the infectious particles (Gaylord and Melnick, 1953;Morgan et al, 1954;Morgan and Wyckoff, 1950). The virus core condensates in this step and the major structural proteins enter the immature envelopes by proteolytic processing (Morgan, 1976;Moss and Rosenblum, 1973).…”

Section: Virion Assembly Maturation and Releasementioning

confidence: 99%