Developmental expression of two antigens associated with mouse intestinal crypts

Abstract

Two monoclonal antibodies were prepared against the duodenal mucosa of fourday‐old mice (MIM‐1/39 and MIM 1/130). The expression of the antigens was associated with the crypts of the small and large intestine in the fetus and adult. MIM‐1/39 was present in epithelial cells of the intervillous areas in the small intestine at 17 and 18 days of gestation; afterwards its expression was detected only in crypt cells from birth to adulthood. Transition from the mouth of the crypts to intestinal villi was abrupt. Expression of MIM‐1/39 was first detected at time of birth in the colon: In the adult, only crypt cells expressed the antigen and goblet cells were negative. Antigen MIM‐1/130 was detected from 16 to 18 days of gestation in the small intestine, in the mesenchymal matrix lying under the intervillous epithelium. After birth, it was present in the pericryptal mesenchymal matrix. This antigen was also expressed at birth in the colon and remained in the pericryptal matrix in the adult. In vivo, multiple injections of an organic extract of rat amniotic fluid to mothers, starting at 14 days of gestation, induced a profound modulation in the pattern of expression of both antigens at 17 days of gestation: The pattern of expression was comparable to that observed at least 5 days after birth in untreated animals. The expression of both antigens before crypt appearance may reflect some molecular differentiation in preparation for the formation of crypts, while their association with differentiated crypts may indicate that they have a role in the maintenance of crypt functional and/or morphological integrity. Finally, the fact that their expression can be modulated experimentally may prove to be a breakthrough for the study of crypt formation. © 1992 Wiley‐Liss, Inc.

Culture of human intestinal epithelial cell using the dissociating enzyme thermolysin and endothelin-3

“…And the presence of these intestinal functional markers in HIEC indicated that these cells have some potential for functional differentiation. As a crypt cell marker, the detection of the MIM-1/39 antigen provided strong evidence that serially passaged HIEC still retained their epithelial character, a feature that was absent in Caco-2 and HIM cells (21,23). This may also indicate that HIEC had the characteristics of crypt cells (31).…”

“…Production of a rabbit-specific anti-mouse intestinal mucosa (MIM) -1/39 antibody has been described elsewhere (21)(22)(23).…”

ERK-associated changes in E2F4 phosphorylation, localization and transcriptional activity during mitogenic stimulation in human intestinal epithelial crypt cells

“…Indeed, the expression of intestinal epithelial-specific keratins [37], of components specific to cell junctions [38], cell cycle-related proteins [9,10,39], as well as typical intestinal cell markers of undifferentiated lower crypt cells [37,39,40] indicate that these cells behave as cells representative of the bottom of the human crypt [41]. Their epithelial cryptal origin was also confirmed by their ability to express the 350-kD crypt cell-specific marker MIM-1/39 [42]. Interestingly, we show herein that although EGF induced a rapid activation of ERK1/2 similarly to serum and LPA, this action was not sufficient for S phase entry as visualized by the absence of pRb hyperphosphorylation, cyclin A protein expression and E2F4 nuclear translocation.…”

Expression and turnover of an interstitial matrix component of the pericryptal sheath in neonatal mice

“…from MIM-1/130 hybridoma cells (125 µg protein/100 µl; group 3) or 100 µl anti-laminin antibodies in saline (106 µg protein/100 µl; group 4) twice daily for 3 days. Anti-laminin (clone LAM-1), a rat anti-mouse monoclonal antibody (ICN ImmunoBiological, Costa Mesa, Calif.) was of the same subclass as Mab-1/130 (Beaulieu et al 1992). This antibody recognizes laminin in a broad array of human and mouse basement membranes in cryostat sections.…”

“…In this regard, the presence of an extracellular matrix molecule, the antigen MIM-1/130, expressed exclusively in the gastrointestinal tract and gallbladder (Beaulieu et al 1992) had raised much interest. At the fine structural level, this new component has been localized in the interstitial matrix around collagen fibrils surrounding fibroblasts of the pericryptal sheath and is absent from the basement membrane (Calvert et al 1994).…”