Developmental expression of the transcription factor zif268 in rat brain

Herms, Jochen; Zurmöhle, Uwe; Schlingensiepen, R.; Brysch, Wolfgang; Schlingensiepen, Karl‐Hermann

doi:10.1016/0304-3940(94)90737-4

Cited by 40 publications

(21 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By contrast to the B cells, in L929 fibroblasts the proximal SRE5 is the major Egr-1 activating binding site, whereas deletion of SRE2-4 or SRE1 did not reduce Egr-1 promoter activity (Figs. 4,5). In 3T3 fibroblasts, the SRE2-4 cluster plays a dominant role in the activation of Egr-1 transcription.…”

Section: Discussionmentioning

confidence: 99%

“…4,5). In resting L929 cells (maintained in medium containing 0.5% FCS) the native Egr-1 promoter activated luciferase production about one-hundred fold when compared to the pGL-2 basic vector (Fig.…”

Section: Egr-1 Promoter Activity In L929 and Nih 3t3 Fibroblastsmentioning

confidence: 96%

“…Egr-1 is a C 2 H 2 zinc-finger transcription factor and is also referred to as krox 24, TIS8, Z225, NGFI-A, zif268 [1][2][3][4][5]. Egr-1 gene products were detected in rheumatoid arthritis (RA) synovial tissue and primary passage RA synovial fibroblasts [6,7].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Analysis of functional elements in the human Egr-1 gene promoter

Aicher

Sakamoto

Hack

et al. 1999

Rheumatology International

View full text Add to dashboard Cite

The early growth response (Egr)-1 gene encoding a zinc-finger transcription factor is transiently induced in many different cell types upon various differentiation signals. However, in synovial fibroblasts of rheumatoid arthritis patients, Egr-1 is constitutively expressed at high levels, and several genes with Egr-1 binding sites in their promoter regions have been associated with disease progression of RA. We analyzed the control of Egr-1 transcription by characterizing those regulatory elements in the Egr-1 promoter that induce Egr-1 expression in fibroblasts. Using reporter gene assays and deletion mutants of the Egr-1 promoter we could demonstrate that Egr-1 transcription is mainly activated by a single serum response element, whereas other transcription factor binding sites, including binding sites for AP-1 or Egr-1, were found to play a minor role. Furthermore, we identified a novel regulatory element in the human Egr-1 promoter similar to a NF kappa-B binding site. Deletion of this element enhanced Egr-1 promoter activity in 3T3 but not in L929 fibroblasts. Stimulation by phorbolester induced only transient Egr-1 expression in 3T3 fibroblasts but a extended expression of Egr-1 in L929 cells. These data suggest that in fibroblasts the most proximal serum response element in the Egr-1 promoter represents the major activation site, whereas binding of the NFkB-like factor may serve as negative regulatory signal for Egr-1 transcription in fibroblasts.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Egr-1 Promoter Activity In L929 and Nih 3t3 Fibroblastsmentioning

confidence: 96%

See 1 more Smart Citation

Analysis of functional elements in the human Egr-1 gene promoter

Aicher

Sakamoto

Hack

et al. 1999

Rheumatology International

View full text Add to dashboard Cite

show abstract

“…Accordingly, EGR1 expression is undetectable in the embryonic nervous system (McMahon et al, 1990; Crosby et al, 1992), but slowly rises throughout postnatal development to reach adult expression levels by postnatal day 17 in the rat hippocampus, for instance (Watson and Milbrandt, 1990; Herms et al, 1994; Beckmann and Wilce, 1997). Interestingly, this progressive increase in EGR1 expression parallels the time of synaptic formation in cortical regions, and in the hippocampal CA1 area, corresponds closely to the period of maximal response to N-methyl-D-aspartate (NMDA) and long-term potentiation (LTP) inducibility (Herms et al, 1994), which underscores the relationship between EGR1 expression and synaptic plasticity.…”

Section: Functions and Regulations Of Egr1mentioning

confidence: 99%

The Role of Early Growth Response 1 (EGR1) in Brain Plasticity and Neuropsychiatric Disorders

Duclot

Kabbaj

2017

Front. Behav. Neurosci.

267

240

View full text Add to dashboard Cite

It is now clearly established that complex interactions between genes and environment are involved in multiple aspects of neuropsychiatric disorders, from determining an individual’s vulnerability to onset, to influencing its response to therapeutic intervention. In this perspective, it appears crucial to better understand how the organism reacts to environmental stimuli and provide a coordinated and adapted response. In the central nervous system, neuronal plasticity and neurotransmission are among the major processes integrating such complex interactions between genes and environmental stimuli. In particular, immediate early genes (IEGs) are critical components of these interactions as they provide the molecular framework for a rapid and dynamic response to neuronal activity while opening the possibility for a lasting and sustained adaptation through regulation of the expression of a wide range of genes. As a result, IEGs have been tightly associated with neuronal activity as well as a variety of higher order processes within the central nervous system such as learning, memory and sensitivity to reward. The immediate early gene and transcription factor early growth response 1 (EGR1) has thus been revealed as a major mediator and regulator of synaptic plasticity and neuronal activity in both physiological and pathological conditions. In this review article, we will focus on the role of EGR1 in the central nervous system. First, we will summarize the different factors influencing its activity. Then, we will analyze the amount of data, including genome-wide, that has emerged in the recent years describing the wide variety of genes, pathways and biological functions regulated directly or indirectly by EGR1. We will thus be able to gain better insights into the mechanisms underlying EGR1’s functions in physiological neuronal activity. Finally, we will discuss and illustrate the role of EGR1 in pathological states with a particular interest in cognitive functions and neuropsychiatric disorders.

show abstract

“…Zif268 is expressed at low levels during development. Its expression gradually increases during the second week after birth in the neocortex and in the hippocampus, where transient expression in the dentate gyrus declines during the third week postnatal (Watson and Milbrandt, 1990;Herms et al, 1994). Basal expression of Zif268 mRNA/protein is rapidly and dramatically reduced in the brain by systemic administration of N-methyl-D-aspartate (NMDA) receptor antagonists as well as in the primary visual cortex after monocular deprivation or dark adaptation, suggesting that naturally occurring afferent synaptic activity regulates the steady-state basal expression of Zif268 (Worley et al, 1991;Chandhuri et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Regulated transcription of the immediate‐early gene Zif268: Mechanisms and gene dosage‐dependent function in synaptic plasticity and memory formation

2002

View full text Add to dashboard Cite

ABSTRACT:The immediate-early gene Zif268 is a member of the Egr family of inducible transcription factors. Data from gene expression studies have suggested that this gene may play a critical role in initial triggering of the genetic machinery that has long been considered a necessary mechanism for maintenance of the later phases of LTP and also for the consolidation or stabilization of long-lasting memories. Until recently, however, the data supporting this assumption have been based primarily on circumstantial evidence, with no direct evidence to suggest that Zif268 is required for long-lasting synaptic plasticity and memory. In this report, we review our own data using Zif268 mutant mice; we show that although the early phase of dentate gyrus LTP is normal in these mice, the later phases are not present, and the ability of the mice to maintain learned information over a 24-h period is deficient. In addition, we present new information showing a task-dependent gene dosage effect in Zif268 heterozygous mice. We show that spatial learning is particularly sensitive to reduced levels of Zif268, as one-half of the complement of Zif268 in heterozygous mice is insufficient to maintain spatial long-term memories.

show abstract

Developmental expression of the transcription factor zif268 in rat brain

Cited by 40 publications

References 20 publications

Analysis of functional elements in the human Egr-1 gene promoter

Analysis of functional elements in the human Egr-1 gene promoter

The Role of Early Growth Response 1 (EGR1) in Brain Plasticity and Neuropsychiatric Disorders

Regulated transcription of the immediate‐early gene Zif268: Mechanisms and gene dosage‐dependent function in synaptic plasticity and memory formation

Contact Info

Product

Resources

About