Developmental expression of the glucose dehydrogenase gene in Drosophila melanogaster.

Abstract

The Gld gene of Drosophila melanogaster is transiently expressed during every stage of development. The temporal pattern of Gld expression is highly correlated with that of ecdysteroids. Exogeneous treatment of third instar larvae with 20-hydroxyecdysone induces the accumulation of Gld mRNA in the hypoderm and anterior spiracular gland cells. During metamorphosis Gld is expressed in a variety of tissues derived from the ectoderm. In the developing reproductive tract, Gld mRNA accumulates in the female spermathecae and oviduct and in the male ejaculatory duct and ejaculatory bulb. These four organs are derived from closely related cell lineages in the genital imaginal disc. Since the expression of Gld is not required for the development of these reproductive structures, this spatial pattern of expression is most likely a fortuitous consequence of a shared regulatory factor in this cell lineage. At the adult stage a high level of the Gld mRNA is only observed in the male ejaculatory duct.

RNAseq-based gene expression analysis ofMelolontha hippocastanihindgut pockets and the surrounding hindgut wall tissue

“…Their concentration in hemolymph increases during each larval stage to quickly drop at each molt, as their amino acid components are used for the synthesis of body tissues (Delobel et al 1993; Chapman 2013). Expression of glucose dehydrogenases is highly correlated with that of 20-hydroxyecdysone, a major insect molting hormone, and is increased during metamorphosis (Cox-Foster et al 1990). HDE arylphorins, Osiris family proteins and glucose dehydrogenases make tempting to speculate that the role of the pockets and, perhaps, their bacterial symbionts, might go beyond insect immunity and nutrition and embrace other aspects of physiology such as development, as it happens in the Riptortus-Burkholderia system (Lee et al 2017) or Aedes mosquitoes (Coon et al 2017).…”