Developmental Expression of Osteopontin (OPN) mRNA in Rat Tissues: Evidence for a Role for OPN in Bone Formation and Resorption

Chen, Jinkun; Singh, Krishna; Mukherjee, Barid B.; Sodek, Jaro

doi:10.1016/s0934-8832(11)80070-3

Cited by 185 publications

(115 citation statements)

References 36 publications

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“…It is also secreted in milk, blood, and urine (bodily fluids). 56 In comparison with its restricted allocation in the normal tissue, OPN is markedly upregulated at sites of inflammation and tissue remodeling. 57,58 OPN exists in two forms, one as a component of the ECM and other as a soluble cytokine.…”

Section: Role Of Osteopontin In Arthritismentioning

confidence: 99%

Molecular targets in arthritis and recent trends in nanotherapy

Roy¹,

Kanwar²,

Kanwar³

2015

IJN

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Due to its severity and increasing epidemiology, arthritis needs no description. There are various forms of arthritis most of which are disabling, very painful, and common. In spite of breakthroughs in the field of drug discovery, there is no cure for arthritis that can eliminate the disease permanently and ease the pain. The present review focuses on some of the most successful drugs in arthritis therapy and their side effects. Potential new targets in arthritis therapy such as interleukin-1β, interleukin-17A, tumor necrosis factor alpha, osteopontin, and several others have been discussed here, which can lead to refinement of current therapeutic modalities. Mechanisms for different forms of arthritis have been discussed along with the molecules that act as potential biomarkers for arthritis. Due to the difficulty in monitoring the disease progression to detect the advanced manifestations of the diseases, drug-induced cytotoxicity, and problems with drug delivery; nanoparticle therapy has gained the attention of the researchers. The unique properties of nanoparticles make them highly attractive for the design of novel therapeutics or diagnostic agents for arthritis. The review also focuses on the recent trends in nanoformulation development used for arthritis therapy. This review is, therefore, important because it describes the relevance and need for more arthritis research, it brings forth a critical discussion of successful drugs in arthritis and analyses the key molecular targets. The review also identifies several knowledge gaps in the published research so far along with the proposal of new ideas and future directions in arthritis therapy.

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Section: Role Of Osteopontin In Arthritismentioning

confidence: 99%

Molecular targets in arthritis and recent trends in nanotherapy

Roy¹,

Kanwar²,

Kanwar³

2015

IJN

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“…Hypertrophic chondrocytes express specific extracellular matrix molecules, such as collagen type X (Col10a1) (Elima et al, 1993;Linsenmayer et al, 1991), and normally undergo a further maturation process. Terminal hypertrophic chondrocytes at the chondro-osseous junction actively express additional molecules, such as matrix metalloproteinase 13 (Mmp13) (Stickens et al, 2004), osteopontin (Opn; also known as Spp1) (Chen et al, 1993) and vascular endothelial growth factor (Vegfa) (Gerber et al, 1999), which are crucial for the invasion of blood vessels, osteoclasts and osteoblast precursors from the perichondrium. Defects in the terminal differentiation of hypertrophic chondrocytes result in severely delayed formation of the primary ossification center (POC) and secondary ossification center (SOC) (Hattori et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Zbtb20 regulates the terminal differentiation of hypertrophic chondrocytes via repression of Sox9

et al. 2015

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The terminal differentiation of hypertrophic chondrocytes is a tightly regulated process that plays a pivotal role in endochondral ossification. As a negative regulator, Sox9 is essentially downregulated in terminally differentiated hypertrophic chondrocytes. However, the underlying mechanism of Sox9 silencing is undefined. Here we show that the zinc finger protein Zbtb20 regulates the terminal differentiation of hypertrophic chondrocytes by repressing Sox9. In the developing skeleton of the mouse, Zbtb20 protein is highly expressed by hypertrophic chondrocytes from late embryonic stages. To determine its physiological role in endochondral ossification, we have generated chondrocyte-specific Zbtb20 knockout mice and demonstrate that disruption of Zbtb20 in chondrocytes results in delayed endochondral ossification and postnatal growth retardation. Zbtb20 deficiency caused a delay in cartilage vascularization and an expansion of the hypertrophic zone owing to reduced expression of Vegfa in the hypertrophic zone. Interestingly, Sox9, a direct suppressor of Vegfa expression, was ectopically upregulated at both mRNA and protein levels in the late Zbtb20-deficient hypertrophic zone. Furthermore, knockdown of Sox9 greatly increased Vegfa expression in Zbtb20-deficient hypertrophic chondrocytes. Our findings point to Zbtb20 as a crucial regulator governing the terminal differentiation of hypertrophic chondrocytes at least partially through repression of Sox9.

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“…Among these, osteopontin (OPN) is originally identified as a major sialylated noncollagenous matrix protein in bone (12). Most studies to date have focused on the role of OPN in the process of mineralization and bone morphogenesis (7,9,42,43,53,57). An extensive study of OPN expression in adult human tissue by Brown et al (6) revealed that the OPN was a major constituent of the luminal surface of epithelial cells in the gastrointestinal tract, gall bladder, pancreas, urinary and reproductive tracts, lung bronchi, mammary and salivary glands, and sweat duct where epithelial cells communicate with the external environment.…”

Section: Introductionmentioning

confidence: 99%

Osteopontin, a Coordinator of Host Defense System: a Cytokine or an Extracellular Adhesive Protein?

Uede

Katagiri

Iizuka

et al. 1997

Microbiology and Immunology

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Developmental Expression of Osteopontin (OPN) mRNA in Rat Tissues: Evidence for a Role for OPN in Bone Formation and Resorption

Cited by 185 publications

References 36 publications

Molecular targets in arthritis and recent trends in nanotherapy

Molecular targets in arthritis and recent trends in nanotherapy

Zbtb20 regulates the terminal differentiation of hypertrophic chondrocytes via repression of Sox9

Osteopontin, a Coordinator of Host Defense System: a Cytokine or an Extracellular Adhesive Protein?

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Developmental Expression of Osteopontin (OPN) mRNA in Rat Tissues: Evidence for a Role for OPN in Bone Formation and Resorption

Cited by 185 publications

References 36 publications

Molecular targets in arthritis and recent trends in&nbsp;nanotherapy

Molecular targets in arthritis and recent trends in&nbsp;nanotherapy

Zbtb20 regulates the terminal differentiation of hypertrophic chondrocytes via repression of Sox9

Osteopontin, a Coordinator of Host Defense System: a Cytokine or an Extracellular Adhesive Protein?

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Molecular targets in arthritis and recent trends in nanotherapy

Molecular targets in arthritis and recent trends in nanotherapy