Developmental expression of LTβR and differential expression in Escherichia coli F18 resistant/sensitive piglets

Abstract

ABSTRACT. We analyzed LTβR mRNA expression in piglets from birth to weaning and compared the differential expression between Escherichia coli F18-resistant and sensitive populations to determine whether this gene could be used as a genetic marker for E. coli F18 resistance. Sutai piglets of different age groups (8, 18, 30, and 35 days; N = 4 each) and piglets demonstrating resistance/sensitivity to E. coli F18 were used. LTβR expression levels were determined by real-time PCR. The LTβR expression levels in the lymph node, duodenum, and jejunum were significantly higher in 8-day-old piglets than in the other age groups (P < 0.01), and the expression levels were significantly 2 R.W. Xia et al. Genetics and Molecular Research 15 (3): gmr.15038377 higher in the lungs of 8-day-old piglets than in 35-day-old piglets (P < 0.01) and 30 day-old piglets (P < 0.05). In liver tissue, the expression level was significantly higher in the 35-day-old piglets than in other age groups (P < 0.01). In the stomach tissue, the expression level was significantly higher in 35-day-old piglets than in 18-day-old piglets (P < 0.05). LTβR expression in the lymph nodes was significantly higher in the resistant group than in the sensitive group (P < 0.01), but there was no significant difference in the other tissues (P > 0.05). These results indicate that 8 days after birth is a crucial stage in the formation of mesentery lymph nodes and immune barriers in pigs, and increased expression of LTβR may be beneficial for developing resistance to E. coli F18.

Lack of LTβR Increases Susceptibility of IPEC-J2 Cells to Porcine Epidemic Diarrhea Virus

“…LTβR is reportedly highly expressed in the lymph nodes, duodenum and jejunum of eight-day-old newborn piglets, which may be beneficial for developing resistance to E. coli F18 in pigs [ 21 ], and IPEC-J2 cells are susceptible to PEDV infection [ 22 ]. As such, we investigated the effects of LTβR on PEDV infections in IPEC-J2 cells.…”

“…LTβR signaling is required for clearance of Salmonella typhimurium in infected gut lumen [ 28 ]. Additionally, very recent data showed that LTβR is highly expressed in the lymph nodes, duodenum and jejunum of eight-day-old newborn piglets, which may be beneficial for developing resistance to E. coli F18 in pigs [ 21 ]. In addition, the role of LTβR in viral infection was investigated.…”

New Insight into the Molecular Mechanism of the FUT2 Regulating Escherichia coli F18 Resistance in Weaned Piglets

“…Furthermore, we identified the differential expression of the FUT2 , FUT3 , TLR5 , TAP2 , IL1β genes in the duodenum, indicating that these genes probably play a crucial role in the resistance to E. coli F18. Our previous studies concentrated on the relationship of immune gene expression and E. coli F18 resistance in pigs [ 4 , 5 , 6 , 7 , 8 ], only a few reports directly on receptor formation showing the effect of the α(1,2) fucosyltransferase 1 ( FUT1 ) gene M307 G/A mutation on the adhesion of E. coli F18 to pig intestinal epithelial cells [ 9 ], but its polymorphism distribution in more than 20 Chinese local pig breeds and wild boar population is extremely skewed [ 10 , 11 , 12 ]. The smallest antigenic determinant of the E. coli F18 receptor is the type 1 H-antigen of the ABO blood group antigens [ 13 ].…”