Developmental Expression of Glial Fibrillary Acidic Protein and Glutamine Synthetase in Serum-Free Aggregating Cell Cultures of Fetal Rat Telencephalon

Monnet‐Tschudi, Florianne; Eng, Lawrence F.; Matthieu, Jean‐Marie; Honegger, Paul

doi:10.1159/000111966

Cited by 11 publications

(1 citation statement)

References 25 publications

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“…2). As the astrocytic enzyme glutamine synthetase (GS) is predominantly located in cell bodies, whereas the cytoskeletal marker GFAP labelled rather astrocytic processes (Monnet-Tschudi et al, 1988), the colocalization of the apoptotic nuclei within the GS-labelled cell bodies were considered for the evaluation. In control cultures, the colocalization of apoptotic nuclei with the astrocytic markers was somewhat lower (38% colocalized with GFAP and 56% colocalized with GS) and the number of unidentifiable cells was higher (32%) than in mercury treated cultures.…”

Section: Astrocytes Undergo Apoptosis After Mehgcl Treatmentsmentioning

confidence: 99%

Induction of apoptosis by mercury compounds depends on maturation and is not associated with microglial activation

Monnet‐Tschudi

1998

J. Neurosci. Res.

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The earliest sign of neurotoxicity observed after exposure of three-dimensional brain cell cultures to low concentrations of mercury compounds is a microglial reaction. We hypothesized that an induction of apoptosis by mercury compounds could be an activating signal of the microglial reaction. Aggregating brain cell cultures of fetal rat telencephalon were treated for 10 days with either mercury chloride or monomethylmercury chloride at noncytotoxic concentrations during two developmental periods: from day 5 to 15, corresponding to an immature stage, and from day 25 to 35 corresponding to a mature stage. Apoptosis was evaluated by the TUNEL technique. It was found that both mercury compounds caused a significant increase in the number of apoptotic cells, but exclusively in immature cultures exhibiting also spontaneous apoptosis. Double staining by the TUNEL technique combined with either neuronal or astroglial markers revealed that the proportion of cells undergoing apoptosis was highest for astrocytes. Furthermore neither an association nor a colocalization was found between apoptotic cells and microglial cells. In conclusion, it appears that the induction of apoptosis by mercury compounds in immature cells is only an acceleration of a spontaneously occurring process, and that it is not a directly related to the early microglial reaction.

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Section: Astrocytes Undergo Apoptosis After Mehgcl Treatmentsmentioning

confidence: 99%

Induction of apoptosis by mercury compounds depends on maturation and is not associated with microglial activation

Monnet‐Tschudi

1998

J. Neurosci. Res.

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Demyelination induced by protein kinase C-activating tumor promoters in aggregating brain cell cultures

Pouly

Storch

Matthieu³

et al. 1997

J. Neurosci. Res.

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The plasticity of mature oligodendrocytes was studied in aggregating brain cell cultures at the period of maximal expression of myelin marker proteins. The protein kinase C (PKC)-activating tumor promoters mezerein and phorbol 12-myristate 13-acetate (PMA), but not the inactive phorbol ester analog 4alpha-PMA, caused a pronounced decrease of myelin basic protein (MBP) content and 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP) activity. In contrast, myelin/oligodendrocyte protein (MOG) content was affected relatively little. Northern blot analyses showed a rapid reduction of MBP and PLP gene expression induced by mezerein, and both morphological and biochemical findings indicate a drastic loss of compact myelin. During the acute phase of demyelination, only a relatively small increase in cell death was perceptible by in situ end labeling and in situ nick translation. Basic fibroblast growth factor (bFGF) also reduced the levels of the oligodendroglial differentiation markers and enhanced the demyelinating effects of the tumor promoters. The present results suggest that PKC activation resulted in severe demyelination and partial loss of the oligodendrocyte-differentiated phenotype.

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Regeneration of the avian retina by retinospheroid technology

Layer

Willbold

1994

Progress in Retinal and Eye Research

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Developmental Expression of Glial Fibrillary Acidic Protein and Glutamine Synthetase in Serum-Free Aggregating Cell Cultures of Fetal Rat Telencephalon

Cited by 11 publications

References 25 publications

Induction of apoptosis by mercury compounds depends on maturation and is not associated with microglial activation

Induction of apoptosis by mercury compounds depends on maturation and is not associated with microglial activation

Demyelination induced by protein kinase C-activating tumor promoters in aggregating brain cell cultures

Regeneration of the avian retina by retinospheroid technology

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