2010
DOI: 10.1128/iai.00473-10
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Developmental Expression of a Trypanosoma cruzi Phosphoinositide-Specific Phospholipase C in Amastigotes and Stimulation of Host Phosphoinositide Hydrolysis

Abstract: Phosphoinositide phospholipase C (PI-PLC) plays an essential role in cell signaling. A unique Trypanosoma cruzi PI-PLC (TcPI-PLC) is lipid modified in its N terminus and localizes to the outer surface of the plasma membrane of amastigotes. We show here that TcPI-PLC is developmentally regulated in amastigotes and shows two peaks of surface expression during the developmental cycle of T. cruzi, the first immediately after differentiation of trypomastigotes into amastigotes and the second before differentiation … Show more

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Cited by 17 publications
(8 citation statements)
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References 25 publications
(33 reference statements)
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“…In conclusion, these works (Hashimoto et al , 2013; Huang et al , 2013) clearly established the presence of a functional IP 3 receptor in T. brucei and T. cruzi, and together with previous reports (Docampo and Pignataro, 1991; Moreno et al , 1992a; Moreno et al , 1992b; Furuya et al , 2000; Okura et al , 2005; de Paulo Martins et al , 2010; Martins et al , 2010), the function of a complete IP 3 /diacylglycerol pathway in trypanosomes.…”
Section: Intracellular Calcium Channels In Parasitic Protozoasupporting
confidence: 86%
See 1 more Smart Citation
“…In conclusion, these works (Hashimoto et al , 2013; Huang et al , 2013) clearly established the presence of a functional IP 3 receptor in T. brucei and T. cruzi, and together with previous reports (Docampo and Pignataro, 1991; Moreno et al , 1992a; Moreno et al , 1992b; Furuya et al , 2000; Okura et al , 2005; de Paulo Martins et al , 2010; Martins et al , 2010), the function of a complete IP 3 /diacylglycerol pathway in trypanosomes.…”
Section: Intracellular Calcium Channels In Parasitic Protozoasupporting
confidence: 86%
“…Similar experiments were done with isolated acidocalcisomes resulting in significant Ca 2+ release by IP 3 (Huang et al , 2013). Previous attempts to show Ca 2+ release by IP 3 had been unsuccessful (Moreno et al , 1992a; Moreno et al , 1992b), which was difficult to explain considering that the parasites do have a phosphoinositide phospholipase C (PI-PLC) (Furuya et al , 2000; Okura et al , 2005; Martins et al , 2010), the enzyme that cleaves phosphatidylinositol 4,5-bisphosphate to generate diacylglycerol and IP 3 , and that IP 3 was detected in both T. cruzi (Moreno et al , 1992b) and T. brucei (Moreno et al , 1992a). When those experiments were done (1991–2) the presence of acidocalcisomes (Vercesi et al , 1994; Docampo et al , 1995) and an acidocalcisome vacuolar H + -PPase in trypanosomes were not known, as they were discovered much latter (Scott et al , 1998; Rodrigues et al , 1999).…”
Section: Intracellular Calcium Channels In Parasitic Protozoamentioning
confidence: 99%
“…Again, we only found TS associated to microvesicles rather than as a soluble protein. Thus, even if differentiating amastigotes shed PI-PLC [ 53 ], the enzymatic activity is not enough to quantitatively hydrolyze the TS GPI-anchor.…”
Section: Resultsmentioning
confidence: 99%
“…It has been previously shown in various types of cells that elevation of IP 3 production, which release Ca 2+ from intracellular stores [24,69] [69] and a robust Ca 2+ release upon exposure to IP 3 BM-, ET-1-, or BK [69]. The elevated intracellular [IP 3 ] can have two possible sources i) the plasma membrane of parasites in intracellular forms, such as amastigotes [70] and ii) IP 3 derived from the plasma membrane of the host changes due to changes in IP 3 synthesis and/or degradation [71,72]. Furthermore, an elevated [IP 3 ] i may provoke an increase in Ca efflux from the SR, which could end in a depletion of intraluminal sarcoplasmic reticulum Ca 2+ content [24,73].…”
Section: Plos Neglected Tropical Diseasesmentioning
confidence: 99%