Developmental expression in the rat cerebellum of SC1, a putative brain extracellular matrix glycoprotein related to SPARC

Mendis, Duane B.; Shahin, Susan; Gurd, James W.; Brown, Ian R.

doi:10.1016/0006-8993(94)91540-7

Cited by 42 publications

(23 citation statements)

References 27 publications

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“…significant levels of SPARC in the normal adult brain in regions enriched in synapses (Mendis et al 1995). In the adult brain, some counteradhesive glycoproteins, such as SPARC and SC1, are associated with the extracellular matrix and are believed to regulate adhesive events at synaptic areas and to prevent further neurite outgrowth (Mendis et al 1994b). We have demonstrated that SPARC is expressed predominantly in ganglion cell soma, and especially in axons, in the bovine retina.…”

Section: Discussionmentioning

confidence: 83%

SPARC Is Expressed by Ganglion Cells and Astrocytes in Bovine Retina

Yan

Sage

Hendrickson

1998

J Histochem Cytochem.

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SUMMARY SPARC (secreted protein, acidic and rich in cysteine) ր osteonectin is a matricellular, counteradhesive glycoprotein that disrupts cell-matrix interactions, interacts with growth factors and components of extracellular matrix, and modulates the cell cycle, but appears to subserve only minor structural roles. SPARC is expressed in a variety of tissues during embryogenesis and remodeling and is believed to regulate vascular morphogenesis and cellular differentiation. Although usually limited in normal adult tissues, SPARC is expressed at significant levels in the adult central nervous system. Using a monoclonal antibody against bovine bone osteonectin, we have determined the localization of SPARC in newborn (3-day-old) and adult (4-8-year-old) normal bovine retinas. SPARC was present in the soma of ganglion cells and strong reactivity was found in ganglion cell axons. Muller cells displayed no immunoreactivity, but SPARC was present in retinal astrocytes that were identified by the astrocyte marker glial fibrillary acidic protein (GFAP). Newborn calf retina showed a staining pattern similar to that of adult retina but exhibited significantly reduced levels of SPARC. Minimal levels of SPARC protein were also detected in some capillaries of the inner retina of both newborn and adult animals, whereas large vessels were negative. The presence of SPARC in the retina was confirmed by Western blotting of retinal extracts. These data indicate that SPARC originating from both neurons and glia of the inner retina may be an important modulator of retinal angiogenesis. The increased expression of SPARC in adult relative to newborn retinal tissue also indicates that SPARC has an ongoing role in the maintainance of retinal functions. (J Histochem Cytochem 46:3-10, 1998)

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Section: Discussionmentioning

confidence: 83%

SPARC Is Expressed by Ganglion Cells and Astrocytes in Bovine Retina

Yan

Sage

Hendrickson

1998

J Histochem Cytochem.

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“…For example, Scl is known to be expressed in astrocytes in the adult rodent brain (Mendis et al 1994;McKinnon and Margolskee 1996), but the Scl promoter region does not show any similarity to astrocyte-specific gene promoters such as the glial fibrillary acidic protein promoter.…”

Section: Genome Research ~ 1079mentioning

confidence: 99%

“…Scl is expressed highly in the nervous system and heart (Johnston et al 1990;Mendis et al 1994;McKinnon and Margolskee 1996) and at lower levels in other tissues, and Sparc, while high in bone, has a more general distribution (Sage et al 1989;Mundlos et al 1992;Mendis et al 1994;Porte et al 1995). Additionally, SC1 has been isolated from the high endothelial venules of the immune system (Girard and Springer 1995).…”

mentioning

confidence: 99%

The exon structure of the mouse Sc1 gene is very similar to the mouse Sparc gene.

McKinnon¹,

Kapsetaki²,

Margolskee³

1996

Genome Res.

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Scl and Sparc are two extracellular proteins sharing similarity in their carboxyi terminus, with 63% identity over a 232-amino-acid region. We have cloned and mapped the genomic locus of mouse Scl. The mouse Scl gene contains 11 exons spanning -35 kb of DNA. The genomic structure [exon/intron boundaries] of Ecl exons 6 to exon 11 is identical to those of the similar portion of the Sparc gene. This suggests that Sd and

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“…SC1 is prominent in the adult rat brain, especially the cerebellum [16]. In the cerebral cortex and hippocampus, SC1 is evident in astrocytes and neurons, whereas in the cerebellum, SC1 exhibits a striking distribution along Bergmann glial fibers [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

“…SC1 (also known as hevin, MAST9, SPARCL1, RAGS1 and ECM2) is an anti-adhesive molecule belonging to the SPARC family of matricellular proteins, which are a subgroup of ECM molecules that mediate interactions between cells and their environment [13][14][15]. SC1 is prominent in the adult rat brain, especially the cerebellum [16]. In the cerebral cortex and hippocampus, SC1 is evident in astrocytes and neurons, whereas in the cerebellum, SC1 exhibits a striking distribution along Bergmann glial fibers [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

The Extracellular Matrix Protein SC1/Hevin Localizes to Multivesicular Bodies in Bergmann Glial Fibers in the Adult Rat Cerebellum

Lively

Brown

2009

Neurochem Res

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SC1 is an extracellular matrix molecule prominent in the mammalian brain. In the cerebellum, SC1 localizes to Bergmann glial cells and perisynaptic glial processes that envelop synapses in the molecular layer. In the present study, confocal microscopy revealed a punctate distribution of SC1 along Bergmann glial fibers that colocalized with the intermediate filament GFAP when fibers were viewed in cross-section. Immunoelectron microscopy showed that the punctate SC1 pattern corresponded to the localization of SC1 in multivesicular bodies situated within Bergmann glial fibers. The pattern of SC1 localization was not disrupted following hyperthermia or pilocarpine-induced status epilepticus. The present study suggests that SC1 protein may reach its destination in perisynaptic glial processes and glial endfeet by transport along Bergmann glial fibers in multivesicular bodies and that this process is preserved following stress.

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Developmental expression in the rat cerebellum of SC1, a putative brain extracellular matrix glycoprotein related to SPARC

Cited by 42 publications

References 27 publications

SPARC Is Expressed by Ganglion Cells and Astrocytes in Bovine Retina

SPARC Is Expressed by Ganglion Cells and Astrocytes in Bovine Retina

The exon structure of the mouse Sc1 gene is very similar to the mouse Sparc gene.

The Extracellular Matrix Protein SC1/Hevin Localizes to Multivesicular Bodies in Bergmann Glial Fibers in the Adult Rat Cerebellum

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