Developmental events in ovules of the ornamental plant Rudbeckia bicolor Nutt

Musiał, Krystyna; Kościńska-Pająk, M.; Śliwińska, Elwira; Joachimiak, Andrzej J.

doi:10.1016/j.flora.2011.07.015

Cited by 11 publications

(13 citation statements)

References 36 publications

(46 reference statements)

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“…However, Galinsoga has a nutritive tissue structure differing from that in other genera of the same subfamily (Asteroideae) that have been studied. As mentioned earlier, Musiał et al (2012) did not record any nutritive tissue in Rudbeckia (however, no documentation from TEM or resin sections was shown), which is allied to Ratibida. We found that the nutritive tissue is only slightly developed in Ratibida as compared to other Asteraceae species that have been studied.…”

Section: Discussionmentioning

confidence: 74%

Comparative Anatomy of Ovules in Galinsoga, Solidago and Ratibida (Asteraceae)

Kolczyk¹,

Stolarczyk²,

Płachno³

2015

Acta Biologica Cracoviensia S. Botanica

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Many Asteraceae species have been introduced into horticulture as ornamental or interesting exotic plants. Some of them, including Solidago and Galinsoga, are now aggressive weeds; others such as Ratibida are not. Special modifications of the ovule tissue and the occurrence of nutritive tissue have been described in several Asteraceae species, including invasive Taraxacum species. This study examined whether such modifications might also occur in other genera. We found that the three genera examined - Galinsoga (G. quadriradiata), Solidago (S. canadensis, S. rigida, S. gigantea) and Ratibida (R. pinnata) - differed in their nutritive tissue structure. According to changes in the integument, we identified three types of ovules in Asteraceae: “Taraxacum” type (recorded in Taraxacum, Bellis, Solidago, Chondrilla), with well-developed nutritive tissue having very swollen cell walls of spongy structure; “Galinsoga” type (in Galinsoga), in which the nutritive tissue cells have more cyto-plasm and thicker cell walls than the other integument parenchyma cells, and in which the most prominent character of the nutritive tissue cells is well-developed rough ER; and “Ratibida” type (in Ratibida), in which the nutritive tissue is only slightly developed and consists of large highly vacuolated cells. Our study and future investigations of ovule structure may be useful in phylogenetic analyses.

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Section: Discussionmentioning

confidence: 74%

Comparative Anatomy of Ovules in Galinsoga, Solidago and Ratibida (Asteraceae)

Kolczyk¹,

Stolarczyk²,

Płachno³

2015

Acta Biologica Cracoviensia S. Botanica

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“…On the other hand, in the ovules of Cynara cardunculus , in the vicinity of the endothelium, the walls of the integument cells remained thin; whereas, thick-walled cells were observed only in the chalazal region of the embryo sac, where specialised nucellar layers formed a hypostase around the podium (Figueiredo et al 2006). It is also worth mentioning that during the analysis of developmental processes in Rudbeckia bicolor , from Asteraceae (Musiał et al 2012), we observed neither special deposition of cell wall material in the inner layers of the integument nor a liquefied zone surrounding the integumentary tapetum (Musiał, unpublished data). Thus, the examples of C .…”

Section: Discussionmentioning

confidence: 82%

“…Than isolated ovaries were dehydrated for 1 h in 70 %, 80 %, 90 % ethanol (one change) and 100 % ethanol (three changes), and incubated (for 1.5 h) in one change of ethanol/methyl salicylate (1:1), one change of ethanol/methyl salicylate (1:3) and two changes of 100 % methyl salicylate (Young et al 1979; Musiał et al 2012). Cleared ovaries were examined with a Nikon Eclipse 80i microscope equipped with Nomarski interference contrast optics.…”

Section: Methodsmentioning

confidence: 99%

“…The periodic acid-Schiff (PAS) reaction was used for visualisation of the total carbohydrates of insoluble polysaccharides (Wędzony 1996). Additionally, material embedded in paraffin (Musiał et al, 2012) or in Technovit 7100 (Kulzer, Germany) (Popielarska-Konieczna et al 2012) was also used for (PAS) reaction. All results were the same: total carbohydrates of insoluble polysaccharides stain magenta to purplish red.…”

Section: Methodsmentioning

confidence: 99%

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Anatomy of ovary and ovule in dandelions (Taraxacum, Asteraceae)

et al. 2012

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The genus Taraxacum Wigg. (Asteraceae) forms a polyploid complex within which there are strong links between the ploidy level and the mode of reproduction. Diploids are obligate sexual, whereas polyploids are usually apomictic. The paper reports on a comparative study of the ovary and especially the ovule anatomy in the diploid dandelion T. linearisquameum and the triploid T. gentile. Observations with light and electron microscopy revealed no essential differences in the anatomy of both the ovary and ovule in the examined species. Dandelion ovules are anatropous, unitegmic and tenuinucellate. In both sexual and apomictic species, a zonal differentiation of the integument is characteristic of the ovule. In the integumentary layers situated next to the endothelium, the cell walls are extremely thick and PAS positive. Data obtained from TEM indicate that these special walls have an open spongy structure and their cytoplasm shows evidence of gradual degeneration. Increased deposition of wall material in the integumentary cells surrounding the endothelium takes place especially around the chalazal pole of the embryo sac as well as around the central cell. In contrast, the integumentary cells surrounding the micropylar region have thin walls and exhibit a high metabolic activity. The role of the thick-walled integumentary layers in the dandelion ovule is discussed. We also consider whether this may be a feature of taxonomic importance.

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“…Studies by Matzk et al (2000), among others, confirmed that the mode of reproduction of horticultural species such as A. cepa, Beta vulgaris, B. napus, and Zea mays was obligate sexual. Using this approach, various kinds of apomixis were detected in populations of fruit crops such as Malus and Rubus (Kron and Husband, 2009;Šarhanová et al, 2012), ornamental species Crataegus and Rudbeckia bicolor (Talent and Dickinson, 2007;Musiał et al, 2012), herbs Hypericum perforatum and Arnica cordifolia (Matzk et al, 2001;Kao, 2007), and different species of Allium (Specht et al, 2001). FCM was also used in supporting microscopic and/or molecular studies on reproductive strategies of Aronia melanocarpa, Townsendia hookeri, and Sorbus spp.…”

Section: Plant Breedingmentioning

confidence: 99%

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

Śliwińska

2018

Folia Horticulturae

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Flow cytometry (FCM) has been used for plant DNA content estimation since the 1980s; however, presently, the number of laboratories equipped with flow cytometers has significantly increased and these are used extensively not only for research but also in plant breeding (especially polyploid and hybrid breeding) and seed production and technology to establish seed maturity, quality and advancement of germination. A broad spectrum of horticultural and medicinal species has been analyzed using this technique, and various FCM applications are presented in the present review. The most common application is genome size and ploidy estimation, but FCM is also very convenient for establishing cell cycle activity and endoreduplication intensity in different plant organs and tissues. It can be used to analyze plant material grown in a greenhouse/field as well asin vitro. Due to somaclonal variation, plant material grown in tissue culture is especially unstable in its DNA content and, therefore, FCM analysis is strongly recommended. Horticultural species are often used as internal standards in genome size estimation and as models for cytometrically studied cytotoxic/anticancer/allelopathic effects of different compounds. With the growing interest in genome modification, increased application of FCM is foreseen.

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Developmental events in ovules of the ornamental plant Rudbeckia bicolor Nutt

Cited by 11 publications

References 36 publications

Comparative Anatomy of Ovules in Galinsoga, Solidago and Ratibida (Asteraceae)

Comparative Anatomy of Ovules in Galinsoga, Solidago and Ratibida (Asteraceae)

Anatomy of ovary and ovule in dandelions (Taraxacum, Asteraceae)

Flow cytometry – a modern method for exploring genome size and nuclear DNA synthesis in horticultural and medicinal plant species

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