Developmental Enhancement of Intracytoplasmic Sperm Injection (ICSI)-Generated Quail Embryos by Phospholipase C.ZETA. cRNA

Mizushima, Shusei; Takagi, Satoshi; Ono, Tamao; Atsumi, Yusuke; Tsukada, Akira; Sakai, Noboru; Shimada, Kiyoshi

doi:10.2141/jpsa.45.152

Cited by 20 publications

(18 citation statements)

References 25 publications

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“…Unfertilized eggs were recovered from the anterior magnum within 1 h after oviposition (Mizushima et al, 2008). Each egg was microinjected with a single ejaculated spermatozoon together with either 50 fmol IP 3 or 2 ng SE.…”

Section: Icsi and Ex Vivo Culturementioning

confidence: 99%

“…The total injected volume was ∼1 nl. All procedures used for ICSI were performed as described by Hrabia et al (2003) and Mizushima et al (2008). Briefly, under a Hoffman modulation contrast microscope (IX70, Olympus), IP 3 or SE solution was first drawn into an injection micropipette, followed by a single ejaculated spermatozoon in the same micropipette.…”

Section: Icsi and Ex Vivo Culturementioning

confidence: 99%

“…By contrast, ICSI has not yet been successful in producing offspring in birds because the natural polyspermic fertilization is difficult to mimic (Hrabia et al, 2003;Mizushima et al, 2009;Mizushima et al, 2010). All quail embryos produced by the injection of a single spermatozoon die before embryonic stage 6 (Mizushima et al, 2008; nomenclature as used in Hamburger and Hamilton, 1951). As mentioned above, during normal fertilization in birds, multiple spermatozoa enter one egg before activation is complete (Fofanova, 1965;Nakanishi et al, 1990;Wishart, 1997).…”

Section: Introductionmentioning

confidence: 99%

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The birth of quail chicks after intracytoplasmic sperm injection

et al. 2014

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Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors -phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) -all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca 2+ rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca 2+ oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.

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Section: Icsi and Ex Vivo Culturementioning

confidence: 99%

Section: Icsi and Ex Vivo Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The birth of quail chicks after intracytoplasmic sperm injection

et al. 2014

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“…In addition, microinjection of an ejaculated sperm of chicken into quail oocyte induces blastodermal development in 22% (Takagi et al, 2007a). However, the development does not go beyond stage VII despite extending the period of culture of quail embryos up to 72 hr after ICSI (Mizushima et al, 2008). Although these results show a single sperm of quail and chicken is capable of fertilizing quail oocyte by ICSI, the rate of blastodermal development is quite limited and the developmental potential is also low.…”

Section: Establishment Of Icsi In Quailmentioning

confidence: 82%

Establishment of Intracytoplasmic Sperm Injection Technique in Japanese Quail and its Possible Application for Poultry Resources and Transgenic Birds

Mizushima

2012

J. Poult. Sci.

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In mammals, a normal offspring can be obtained even from infertile male by intracytoplasmic sperm injection (ICSI). Although ICSI technique has permitted significant progress in clinical practice in humans and mouse to date, it has been established recently in birds. In addition, efficiency of fertility and developmental rates has been low and no chick has been produced by in vitro fertilization and culture. Furthermore, polyspermic fertilization and subsequent normal developmental processes remains unknown. The enhancement of fertility and developmental rates is the first step in the avian ICSI system to be applied for protection of endangered species and production of transgenic and clone birds. This review paper describes (1) the establishment of ICSI technique in Japanese quail, (2) molecular mechanisms whereby polyspermy activates development of quail oocyte, (3) improvement of ICSI efficiency by phospholipase Cζ cRNA. Also, possible application of ICSI for avian sex manipulation and transgenic birds was summarized.

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“…The injection of chicken PLCζ RNA into mice oocytes induced egg activation (Dong et al, 2000). The injection of quail sperm and PLCζ RNA or of higher concentration of PLCζ RNA alone has proven its efficiency to induce the formation of the quail female pronucleus (Mizushima et al, 2008;. The existence of a positive dose effect of PLCζ RNA on the rate of early embryo development lead to suggest that polyspermy could help to increase the rate of PLCζ liberated in the oocyte and needed for an optimal activation of the egg.…”

Section: Contribution Of Sperm To the Fertilization Processmentioning

confidence: 99%

Biological Features of the Avian Male Gamete and their Application to Biotechnology of Conservation

Blesbois¹

2012

J. Poult. Sci.

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Male gamete biology shows specific features in birds that contribute to the reproductive strategy of flying animals adapted to highly variable environmental conditions. In this paper, we review the main characteristics of bird sperm, their selection and storage in the highly specialized female oviduct, specific features of polyspermic fertilization, and the consequences of these biological adaptations on semen biotechnology. Among other features, the storage of sperm in specific oviducal glands is a key factor to increase reproductive "freedom" but is also a critical point for the success of semen in vitro storage.

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Developmental Enhancement of Intracytoplasmic Sperm Injection (ICSI)-Generated Quail Embryos by Phospholipase C.ZETA. cRNA

Cited by 20 publications

References 25 publications

The birth of quail chicks after intracytoplasmic sperm injection

The birth of quail chicks after intracytoplasmic sperm injection

Establishment of Intracytoplasmic Sperm Injection Technique in Japanese Quail and its Possible Application for Poultry Resources and Transgenic Birds

Biological Features of the Avian Male Gamete and their Application to Biotechnology of Conservation

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