Developmental Effects of Ambient UV-B Light and Landfill Leachate in Rana blairi and Hyla chrysoscelis

Brüner, M.; Shipman, Paul A.; Rao, Mahesh; Bantle, John A.

doi:10.1006/eesa.2002.2248

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…Under high UVB radiation many embryonic L. peronii hatched with pronounced spinal flexure. UVB‐induced spinal flexure was first observed in the western toad, Bufo boreas and has been described in many species since (Worrest & Kimeldorf, 1975; Bruner et al ., 2002). Growth of larval L. peronii was also reduced under high UVB exposure which is similar to other studies of UVB effects on larval amphibians (Belden & Blaustein, 2002; Blaustein et al ., 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Amphibian sensitivity to UVB varies across taxa, from the highly sensitive alpine newt, Triturus alpestris (Nagl & Hofer, 1997), to the highly resistant pacific tree frog, Hyla regilla (Blaustein et al ., 1994; Hatch & Blaustein, 2003). While the consequences of lethal UVB damage for natural populations are obvious, sublethal effects may also have important implications (Belden et al ., 2000; Blaustein et al ., 2000; Kats et al ., 2000; Bruner et al ., 2002). Effects of UVB on growth, locomotion and behaviour of amphibians may convey a significant reduction in survival in the natural environment.…”

Section: Introductionmentioning

confidence: 99%

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Cooler temperatures increase sensitivity to ultraviolet B radiation in embryos and larvae of the frog Limnodynastes peronii

Uitregt

Wilson

Franklin

2007

Global Change Biology

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Recent studies suggest that complex interacting processes are driving global amphibian declines. Increased ultraviolet B (UVB) radiation in the solar spectrum associated with ozone depletion has been implicated in declines, and evidence suggests that the effects of UVB radiation on amphibians may be greater at cooler temperatures. We tested the thermal sensitivity of UVB effects on amphibians in a controlled factorial experiment using the striped marsh frog, Limnodynastes peronii as a model species. We compared survival, growth and locomotor performance of embryonic and larval L. peronii reared under low and high UVB exposures at both 20 and 30 1C. Embryonic and larval L. peronii proved extremely sensitive to UVB damage and exhibited greater sensitivity at 20 1C compared with 30 1C. Embryonic survival to Gosner stage 25 was unaffected by UVB exposure at 30 1C, but at 20 1C survival was reduced to 52% under high UVB. Larval survival exhibited a similar trend. At 20 1C, all tadpoles survived under low UVB, whereas under high UVB there was 100% mortality after 15 days of exposure. At 30 1C, 86% survived under low UVB, but only 46% survived under high UVB. Sublethal effects such as, embryonic malformation, retarded larval growth and reduced larval swimming performance were also greater at 20 1C compared with 30 1C. Our results strongly indicate that UVB damage in amphibians is markedly increased at cooler temperatures. Thus, populations of UVB sensitive species occurring at cold climates may be at greater risk of declines due to increased solar UVB radiation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cooler temperatures increase sensitivity to ultraviolet B radiation in embryos and larvae of the frog Limnodynastes peronii

Uitregt

Wilson

Franklin

2007

Global Change Biology

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“…For example, Plaza and Lambertucci (2017) showed that land ll negatively affects amphibian´s tness, and can even produce high mortality due to contaminants. Similarly, Bruner et al (2002) in an experimental study detected that land ll leachate caused malformations and high mortality in Rana blairi and Hyla chrysoscelis.…”

Section: Introductionmentioning

confidence: 86%

How landfills affect amphibian’s morphology? The case of the invasive species Rhinella marina

Aguilar-Peralta

Prieto-Dueñas

Borges

et al. 2022

Preprint

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Landfills have been identified as one of the main threats to wildlife, such as amphibians, since landfills as they contaminate the aquatic and terrestrial habitat and act as stressors, negatively affecting the development and morphology of amphibians. However, few studies have been realized to know the morphological variation of anurans under environmental disturbances. In this study, we compared the morphological patterns of Rhinella marina in contrasting habitats: a temperate forest and a landfill, in the San Martín de las Cañas dam, Jalisco, Mexico. We analyzed morphology, body traits size and allometric patterns. The Body shape of the landfill toads was more elongated and thinner than that of the forest toads. We found that toads from the forest had greater sizes in most of the morphological traits in comparison to toads from the landfill. Allometric patterns showed that most of the morphological characters had a negative allometric patterns in many traits in toads from both conditions. Rhinella marina has showed affinity for disturbed areas or sites around human settlements, and persists in habitats altered by urbanization. However, our results showed an increase in stress levels in toads that inhabit landfills.

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“…The frog embryo teratogenesis assay: Xenopus (FETAX) is a 96-h acute toxicity assay determining lethality, morphologic alterations and minimum growth-inhibitory concentration. It has been used in over 100 studies focused on assessment of physical substances, chemicals, mixtures and environmental water and soil samples (Boga et al, 2008;Brausch et al, 2010;Bruner et al, 2002;Hoke and Ankley, 2005;Prati et al, 2000). This assay uses the African clawed frog X. laevis due to its suitable biological characteristics for captive breeding (short reproductive cycle and fully aquatic habitat) and the fact that it is the most extensively studied and best known research amphibian (Robert and Ohta, 2009).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of diclofenac-induced embryotoxicity and teratogenesis in Xenopus laevis and Lithobates catesbeianus, using the frog embryo teratogenesis assay: Xenopus (FETAX)

Cardoso-Vera

Islas-Flores

SanJuan-Reyes

et al. 2017

Science of The Total Environment

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Water is an increasingly deteriorated, limited natural resource due to population increase and industrialization. Also, the widespread use of pharmaceuticals in modern society leads to their presence in domestic, hospital and industrial effluents. Due to their analgesic properties, some of the most commonly used pharmaceuticals are nonsteroidal anti-inflammatory drugs (NSAIDs). High concentrations of one these products, diclofenac (DCF), have been detected in effluents and water bodies of different countries, including Mexico. Diverse studies show that trace amounts (ngL to μgL) of this compound induce toxicity on aquatic organisms such as algae, microcrustaceans and fish. However, studies on its potential toxicity during development in species of commercial interest such as the American bullfrog Lithobates catesbeianus are scarce. The present study aimed to evaluate DCF-induced teratogenesis and embryotoxicity in Xenopus laevis and L. catesbeianus, a species marketed as a nutritional meat source in Mexico, using the frog embryo teratogenesis assay: Xenopus (FETAX). Oocytes in mid-blastula transition were exposed for 96h to 1, 4, 8, 16, 32 and 62.5mgDCFL. The criteria evaluated were mortality, malformation and growth inhibition. The teratogenic index was 4.2 in L. catesbeianus, three-fold higher than the reference limit (1.5), and 3.9 in X. laevis. Diclofenac induced diverse malformations in both species, the most frequent of these being axial malformations in the tail and notochord, edema and stunted growth. Results indicate that DCF is a potentially teratogenic compound and is toxic during development in X. laevis and L. catesbeianus, a species which, due to its sensitivity, can be used to evaluate the toxicity of pharmaceutical products, using FETAX.

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Developmental Effects of Ambient UV-B Light and Landfill Leachate in Rana blairi and Hyla chrysoscelis

Cited by 5 publications

References 37 publications

Cooler temperatures increase sensitivity to ultraviolet B radiation in embryos and larvae of the frog Limnodynastes peronii

Cooler temperatures increase sensitivity to ultraviolet B radiation in embryos and larvae of the frog Limnodynastes peronii

How landfills affect amphibian’s morphology? The case of the invasive species Rhinella marina

Comparative study of diclofenac-induced embryotoxicity and teratogenesis in Xenopus laevis and Lithobates catesbeianus, using the frog embryo teratogenesis assay: Xenopus (FETAX)

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