Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer

Galli, Cesare; Colleoni, Silvia; Duchi, Roberto; Lagutina, Irina; Lazzari, Giovanna

doi:10.1016/j.anireprosci.2006.10.011

Cited by 141 publications

(141 citation statements)

References 37 publications

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“…The maturation rate is in agreement with data from other experimental laboratories engaged in the field of equine ICSI where polar body extrusion rates of about 63% (Choi et al 2004) and 60% (Galli et al 2007) The first foal after ICSI was born in the USA (Squires et al 1996). In the following years this result could not be repeated.…”

Section: Resultssupporting

confidence: 80%

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ICSI – A biotechnological method to produce equine embryos in vitro

Alm¹,

Vernunft²,

Torner³

et al. 2010

PHK

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Section: Resultssupporting

confidence: 80%

“…The development of new culture media in the last years led to development of blastocysts in a complete in vitro system (Choi et al 2006, Galli et al 2007). …”

Section: Resultsmentioning

confidence: 99%

ICSI – A biotechnological method to produce equine embryos in vitro

Alm¹,

Vernunft²,

Torner³

et al. 2010

PHK

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“…Only motile spermatozoa were selected for ICSI and immobilised by two or three Piezo pulses before injection. The resulting presumptive zygotes were cultured in synthetic oviductal fluid (SOF) supplemented with minimum essential medium (MEM) essential and non-essential amino acids, glutamine and BSA and examined for cleavage on Day 2 after injection; those showing development to the 2-cell stage or more (26/32; 81.25%) were cultured in vitro for an additional 6 days, with the culture medium refreshed on Days 4 and 6 (Galli et al 2007). On Day 8 of development, eight of the 12 resulting embryos (12/32; 37.5%) were washed twice in PBS containing 0.1% polyvinyl alcohol (PVA; Sigma-Aldrich Chemicals) and transferred individually to DNase-RNase-free tubes in RLT buffer (Qiagen, Milan, Italy), snap-frozen in liquid nitrogen and stored at À808C.…”

Section: Embryo Collectionmentioning

confidence: 99%

“…Despite the relatively low per oocyte success rates (18%-36% of recovered oocytes develop into a transferable blastocyst; Hinrichs et al 2005;Galli et al 2007), during the past 5 years IVP has become a commercially viable means of breeding horses. The increasing popularity of IVP despite the relatively high costs is explained by the number of transferable blastocysts per ovum pick-up (OPU) session (0.6-0.8; Galli et al 2014), which compares favourably with embryo flushing in the field.…”

Section: Introductionmentioning

confidence: 99%

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Maternal age and in vitro culture affect mitochondrial number and function in equine oocytes and embryos

Hendriks¹,

Colleoni²,

Galli³

et al. 2015

Reprod. Fertil. Dev.

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Abstract. Advanced maternal age and in vitro embryo production (IVP) predispose to pregnancy loss in horses. We investigated whether mare age and IVP were associated with alterations in mitochondrial (mt) DNA copy number or function that could compromise oocyte and embryo development. Effects of mare age (,12 vs $12 years) on mtDNA copy number, ATP content and expression of genes involved in mitochondrial replication (mitochondrial transcription factor (TFAM ), mtDNA polymerase g subunit B (mtPOLB) and mitochondrial single-stranded DNA-binding protein (SSB)), energy production (ATP synthase-coupling factor 6, mitochondrial-like (ATP-synth_F6)) and oxygen free radical scavenging (glutathione peroxidase 3 (GPX3)) were investigated in oocytes before and after in vitro maturation (IVM), and in early embryos. Expression of TFAM, mtPOLB and ATP-synth-F6 declined after IVM (P , 0.05). However, maternal age did not affect oocyte ATP content or expression of genes involved in mitochondrial replication or function. Day 7 embryos from mares $12 years had fewer mtDNA copies (P ¼ 0.01) and lower mtDNA : total DNA ratios (P , 0.01) than embryos from younger mares, indicating an effect not simply due to lower cell number. Day 8 IVP embryos had similar mtDNA copy numbers to Day 7 in vivo embryos, but higher mtPOLB (P ¼ 0.013) and a tendency to reduced GPX3 expression (P ¼ 0.09). The lower mtDNA number in embryos from older mares may compromise development, but could be an effect rather than cause of developmental retardation. The general down-regulation of genes involved in mitochondrial replication and function after IVM may compromise resulting embryos.

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The equine oocyte: Factors affecting meiotic and developmental competence

Hinrichs

2010

Molecular Reproduction Devel

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SUMMARYThere is currently much interest in assisted reproduction techniques in the horse, however, many aspects of oocyte maturation, fertilization, and embryo development in the horse differ from those in other species. Because of the close attachment of the equine oocyte to the follicle wall, scraping of the follicle is the most effective method for oocyte recovery. A notable feature of equine oocytes is that those with expanded cumuli (Ex oocytes), which originate from atretic follicles, have higher meiotic competence (ability to mature to metaphase II in vitro) than do oocytes with compact cumuli (Cp oocytes). Cp oocytes originate in viable follicles but are largely juvenile. Recovery and culture of equine oocytes immediately after slaughter yields a higher maturation rate than that obtained from oocytes after ovary storage; this is related to damage to chromatin in Cp oocytes during storage. In contrast, developmental competence (rate of blastocyst development in vitro) is higher in oocytes recovered from the ovary after a delay. The optimum duration of maturation varies based on cumulus morphology and time of recovery from the ovary, but there is no difference in developmental competence between Ex and Cp oocytes. Because standard in vitro fertilization is not repeatable in the horse, oocyte transfer (surgical transfer of oocytes to the oviducts of inseminated mares) has been developed to allow fertilization of isolated oocytes. Fertilization in vitro may be achieved using intracytoplasmic sperm injection; culture of injected oocytes in a medium with high glucose can yield over 30% blastocyst development.Mol. Reprod. Dev. 77: 651-661,

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Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer

Cited by 141 publications

References 37 publications

ICSI – A biotechnological method to produce equine embryos in vitro

ICSI – A biotechnological method to produce equine embryos in vitro

Maternal age and in vitro culture affect mitochondrial number and function in equine oocytes and embryos

The equine oocyte: Factors affecting meiotic and developmental competence

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