Developmental changes of Islet-1 and its co-localization with pituitary hormones in the pituitary gland of chick embryo by immunohistochemistry

Liu, Jiali; He, Yang; Xiu-mei, Wang; Zheng, Xing; Cui, Sheng

doi:10.1007/s00441-005-0021-3

Cited by 15 publications

(8 citation statements)

References 33 publications

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“…Hematoxylin and eosin staining was performed as previously described [42]. Briefly, sections were dewaxed, rehydrated, stained with hematoxylin, incubated in bluing solution, counterstained with eosin, dehydrated, and equilibrated with xylene.…”

Section: Methodsmentioning

confidence: 99%

LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3

Pan

Wei

et al. 2014

BMC Biol

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BackgroundAbnormalities in pyloric development or in contractile function of the pylorus cause reflux of duodenal contents into the stomach and increase the risk of gastric metaplasia and cancer. Abnormalities of the pyloric region are also linked to congenital defects such as the relatively common neonatal hypertrophic pyloric stenosis, and primary duodenogastric reflux. Therefore, understanding pyloric development is of great clinical relevance. Here, we investigated the role of the LIM homeodomain transcription factor Isl1 in pyloric development.ResultsExamination of Isl1 expression in developing mouse stomach by immunohistochemistry, whole mount in situ hybridization and real-time quantitative PCR demonstrated that Isl1 is highly expressed in developing mouse stomach, principally in the smooth muscle layer of the pylorus. Isl1 expression was also examined by immunofluorescence in human hypertrophic pyloric stenosis where the majority of smooth muscle cells were found to express Isl1. Isl1 function in embryonic stomach development was investigated utilizing a tamoxifen-inducible Isl1 knockout mouse model. Isl1 deficiency led to nearly complete absence of the pyloric outer longitudinal muscle layer at embryonic day 18.5, which is consistent with Gata3 null mouse phenotype. Chromatin immunoprecipitation, luciferase assays, and electrophoretic mobility shift assays revealed that Isl1 ensures normal pyloric development by directly targeting Gata3.ConclusionsThis study demonstrates that the Isl1-Gata3 transcription regulatory axis is essential for normal pyloric development. These findings are highly clinically relevant and may help to better understand pathways leading to pyloric disease.

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Section: Methodsmentioning

confidence: 99%

LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3

Pan

Wei

et al. 2014

BMC Biol

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“…The ovaries were collected at 15.5, 17.5, 19.5 dpc, and 1, 3, 7 dpp for CYP51 immunostrain as described by previous reports (Liu et al, 2005). Briefly, the tissue was fixed, embedded, and 5 mm sections were cut by a microtome.…”

Section: Immunochemistrymentioning

confidence: 99%

Lanosterol metabolic product(s) is involved in primordial folliculogenesis and establishment of primordial folliclepool in mouse fetal ovary

Zhang

Xie

et al. 2008

Molecular Reproduction Devel

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The primordial follicles present in neonatal ovary represent the fecundity of a female throughout her reproductive life. Germ cell meiosis and apoptosis are two important events during primordial folliculogenesis. In this study, through focusing on the cytochrome P450 lanosterol 14 alphademethylase (CYP51) and its lanosterol metabolic product(s), we explored the possible regulatory mechanism of the initiation of germ cell meiosis and primordial follicle formation. The expression of CYP51 could be detected in both oocytes and granulosa cells during primordial folliculogenesis by immunochemistry. RS21745, which leads to the reduction of lanosterol metabolic product(s) level, inhibited the primordial follicle formation in a dose-dependent manner, and thus postpone the establishment of the primordial follicle pool when the mouse fetal ovaries were cultured in serum-free medium. In contrast, the number of primordial follicle increased significantly with the accumulation of the lanosterol metabolic products caused by 0.025, 0.0625, and 0.125 microM AY9944-A-7 supplements. AY9944-A-7 also up-regulated the expression of meiotic diplotene stage marker gene msy2 and primordial follicle formation regulatory gene fig-alpha. Furthermore, AY9944-A-7 decreased the expression of apoptosis gene bax and significantly prevented oocyte apoptosis from 15.37 +/- 1.97% to 3.68 +/- 0.27% (P < 0.01) in neonatal ovary in vitro. In conclusion, our results indicate that lanosterol metabolic product(s) is involved in the primordial folliculogenesis by regulating the oocyte meiosis and apoptosis.

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“…Islet‐1 and Lim‐3 belong to LIM homeodomain transcription factors. Both Islet‐1 and Lim‐3 play critical roles in cell determination, proliferation and differentiation within the nervous system [19, 23–27], heart and foregut [28], inner ear [29], thymus [30, 31], retina [32], pituitary gland [33–36], adrenal medulla [37] and pancreatic cell [38]. Islet‐1 and Lim‐3 have also been detected in the developing pineal gland of the rat [16], Xenopus [19], mouse [20], zebrafish [17, 21] and amphioxus [18, 22].…”

Section: Introductionmentioning

confidence: 99%

LIM homeodomain proteins Islet‐1 and Lim‐3 expressions in the developing pineal gland of chick embryo by immunohistochemistry

Zhang

Liu

et al. 2006

Journal of Pineal Research

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LIM homeodomain proteins Islet-1 and Lim-3 expression and their role in nervous tissue and endocrine glands have been reported; however, nothing is known concerning Islet-1 and Lim-3 expression in the developing pineal gland of the chick embryo. The aim of the present study was to determine the ontogeny of Islet-1 and Lim-3 expression in the developing pineal gland of chick embryo using immunohistochemistry. The results showed that Islet-1 and Lim-3 immunopositive cells were first detected in the pineal evagination of chick embryos at day 4 (E4) and E4.5 of incubation, respectively. In the later developing stages, both Islet-1 and Lim-3 immunopositive cells were consistently detected in the follicular and parafollicular pinealocytes throughout the pineal gland. The relative percentage of Islet-1 immunopositive (Islet-1+) cells relative to the total cells was about 6% at E4.5, and then kept increasing (P < 0.05) and reached about 40% by E12.5; this was followed by no obvious changes until the chicks were newly hatched. The change in Lim-3 immunopositive (Lim-3+) cell number was parallel to that of Islet-1, although Lim-3+ cell were significantly fewer than Islet-1+ cell numbers from E4.5 to E8.5 (P < 0.05). Dual immunohistochemical staining results showed that almost all the Lim-3+ cells expressed Islet-1 at every stage examined, and about 90% of Islet-1+ cells were proliferating cell nuclear antigen negative. These results suggest that both Islet-1 and Lim-3 may be involved in regulating the development and functional maturation of the pineal gland, although further studies are required in elucidating the functional roles of Islet-1 and Lim-3 and the related mechanisms.

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Developmental changes of Islet-1 and its co-localization with pituitary hormones in the pituitary gland of chick embryo by immunohistochemistry

Cited by 15 publications

References 33 publications

LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3

LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3

Lanosterol metabolic product(s) is involved in primordial folliculogenesis and establishment of primordial folliclepool in mouse fetal ovary

LIM homeodomain proteins Islet‐1 and Lim‐3 expressions in the developing pineal gland of chick embryo by immunohistochemistry

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