Developmental Changes of Free Amino Acids in Bovine Fetal Fluids With Gestational Age and the Interrelationships Between the Amino Acid Concentrations in the Fluid Compartments

Baetz, A L; Hubbert, William T.; Graham, Caroline

doi:10.1530/jrf.0.0440437

Cited by 20 publications

(9 citation statements)

References 7 publications

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“…As in other mammals (Young & Prenton, 1969 ;Young & Hill, 1973 ;Baetz, Hubbert & Graham, 1975), the concentration of total -amino acids was highest in the fetal plasma, indicating an enhanced protein turnover rate in the fetus. Since the free amino acids need to be transferred across the placental membranes, those in the maternal plasma are presumably moved against a concentration gradient by active processes related to the fetal metabolism (Young & Hill, 1973).…”

Section: Discussionsupporting

confidence: 52%

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Tam¹,

Chan²

1977

Reproduction

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The changes in the biochemical composition of maternal plasma, fetal plasma and fetal extraembryonic fluid were studied in Wistar rats from Days 13 1/2 to 21 1/2 of gestation. During this period, there was an increase in maternal plasma volume and total blood volume. The total protein concentration decreased, but little change was observed in the protein pattern, total alpha-amino nitrogen, urea nitrogen and sodium concentration. In fetal plasma, total protein concentration increased progressively with a prominent increase in albumin near term. The albumin:globulin ratio was lower than that in maternal plasma because of the presence of two alpha-fetoproteins and four transferrins. The concentration of alpha-amino nitrogen was higher than that in maternal plasma but the urea nitrogen level did not change significantly. The fetal extraembryonic fluid reflected the changes in composition of fetal plasma, especially in the protein pattern, and concentrations of alpha-amino nitrogen and sodium. Total protein and urea nitrogen increased markedly in concentration near term but there was reduction in the fluid volume at this time.

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Section: Discussionsupporting

confidence: 52%

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Tam¹,

Chan²

1977

Reproduction

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“…The AAs in the amniotic fluid mainly derive from maternal and fetal circulation through the placenta [22,23]; they are transported across the placenta by active transport mediated by numerous different AA transporters [24]. In the present study, the lower concentration of individual AAs in the amniotic fluid of V/T pregnancies could be caused by an impaired placental vascularization.…”

Section: Discussionmentioning

confidence: 54%

Differences in amniotic amino acid concentrations between pregnancies obtained with transfer of vitrified thawed in vitro–produced embryos and with natural mating in sheep

et al. 2015

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“…One of the major differences between defined medium and medium supplemented with serum is that the defined medium lacks ornithine. Although variable between serum lots the concentration of ornithine in serum has been estimated to be about 5 x 10-6 M (Baetz, Hubbert and Graham 1975). In studies employing defined media (Sertich, Glass, Fuller and Gerner 1986;Linebaugh and Rillema 1984) the cells can only generate ornithine from arginine, which is a component of medium 199.…”

Section: Discussionmentioning

confidence: 99%

Polyamine Influences on the Prolactin Stimulation of Phosphoprotein Synthesis in Hydroxyurea Synchronized MCF-7 Human Mammary Epithelial Cells

Be¹,

Rillema²

1991

Horm Metab Res

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The actions of prolactin on the rate of synthesis of an isoelectrically precipitable (pH 4.6) phosphoprotein fraction of the MCF-7 human mammary epithelial cell line were determined in cells synchronized at the G1:S interphase of the cell cycle employing hydroxyurea in a serum-free defined medium. Cells not allowed to enter the S-phase of DNA replication, by maintaining hydroxyurea in the incubation medium, exhibited an increased rate of [3H] leucine incorporation into the isoelectrically precipitable phosphoprotein fraction when exposed to prolactin and 1-5 mM spermidine. Cells released from the hydroxyurea induced synchrony exhibited an increased rate of [3H] leucine incorporation in response to prolactin when ornithine, putrescine, or spermidine were present. The polyamine spermine was ineffective in allowing prolactin's action on phosphoprotein synthesis. In synchronized cells released from the hydroxyurea block, prolactin was shown to effect an increased rate of phosphoprotein synthesis at the posttranscriptional G1 stage of the cell cycle. All prolactin responses were attained with physiological concentrations of the hormone. During and subsequent to the synchrony period with hydroxyurea, the presence or absence of insulin was found to be useful for the "staging" of the cell cycle to maintain cell synchrony and obtain prolactin effects on phosphoprotein synthesis.

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Developmental Changes of Free Amino Acids in Bovine Fetal Fluids With Gestational Age and the Interrelationships Between the Amino Acid Concentrations in the Fluid Compartments

Cited by 20 publications

References 7 publications

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Differences in amniotic amino acid concentrations between pregnancies obtained with transfer of vitrified thawed in vitro–produced embryos and with natural mating in sheep

Polyamine Influences on the Prolactin Stimulation of Phosphoprotein Synthesis in Hydroxyurea Synchronized MCF-7 Human Mammary Epithelial Cells

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