Developmental Changes in the Expression of the Growth Hormone Receptor Messenger Ribonucleic Acid and Protein in the Bovine Ovary1

Kölle, Sabine; Sinowatz, Fred; Boie, Gudrun; Lincoln, Doug

doi:10.1095/biolreprod59.4.836

Cited by 85 publications

(62 citation statements)

References 36 publications

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“…Cleavage rate was evaluated on day-2 post insemination. maturation [7,12,17]. Moreover, GH stimulates in vitro oocyte maturation as well as embryo development in cattle [3][4][5].…”

Section: Methodsmentioning

confidence: 99%

In vitro maturation and fertilisation of bovine oocytes in relation to GH gene polymorphism (Leu/Val)

Lechniak¹,

Adamowicz²,

Stanisławski³

et al. 2002

Reprod. Nutr. Dev.

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-The present study describes the analysis of the associations between the growth hormone gene polymorphism (Leu/Val) and oocyte maturation and in vitro fertilisation in cattle. Two independent experiments were carried out. In the first one, oocytes were collected from 49 single ovaries, matured in vitro, measured and cytogenetically analysed. One ovary was considered as a donor. The procedure of the donor's genotyping at the GH locus was based on DNA extracted from the granulosa cells. The GH genotype did not influence the oocyte diameter nor the number of oocytes collected, which were selected for maturation and matured. An unreduced chromosome number was found in 8.8% of the cells at the second metaphase stage and 42.9% of the donors. This anomaly was observed in all genotype groups with a higher frequency in the VV cows (P < 0.01). In the second experiment, the oocytes collected from 72 single ovaries were matured and fertilised in vitro. The GH genotype of a donor did not influence the number of zygotes cleaved on day-2. It has to be mentioned, that due to the low frequency of the VV genotype (0.03), the results of the present study should be treated as preliminary and need further analysis. oocyte / in vitro maturation / GH polymorphism Reprod. Nutr. Dev. 42 (2002) 275-280 275

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“…Cleavage rate was evaluated on day-2 post insemination. maturation [7,12,17]. Moreover, GH stimulates in vitro oocyte maturation as well as embryo development in cattle [3][4][5].…”

Section: Methodsmentioning

confidence: 99%

In vitro maturation and fertilisation of bovine oocytes in relation to GH gene polymorphism (Leu/Val)

Lechniak¹,

Adamowicz²,

Stanisławski³

et al. 2002

Reprod. Nutr. Dev.

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“…In the presence of RA, the growth hormone (GH) receptor (GHr) gene increases transcription (Ohlsson et al 1993, Slootweg et al 1996. The transcript of the GHr was demonstrated in oocytes, follicular cells and the corpus luteum of the bovine ovary (Kolle et al 1998), and GH was found to stimulate the bovine oocyte during IVM via cumulus cells (Izadyar et al 1997, Kolle et al 2003.…”

Section: Embryos Transferred Pregnancy (%)mentioning

confidence: 99%

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Hidalgo¹,

Díez²,

Duque³

et al. 2005

Reproduction

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Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and culturedin vitro.Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.

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“…Receptores de somatotropina e IGF-1 têm sido encontrados em oócitos bovinos imaturos e nas células da granulosa e do cumulus Spicer & Stewart, 1996;Izadyar et al, 1997;Kölle et al, 1998;Yoshida et al, 1998;Lonergan et al, 2000). A adição de rbST (Izadyar et al, 1997(Izadyar et al, , 1998) ou IGF-1 (Herrler et al, 1992;Markkula & Makarevich, 2001) ao meio de maturação in vitro tem aumentado o número de mórulas e de blastocistos.…”

Section: Resultsunclassified

Efeito da somatotropina na população folicular, recuperação de oócitos e produção in vitro de embriões em vacas Gir

et al. 2007

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RESUMO -Avaliou-se o efeito da pré-estimulação ovariana com somatotropina bovina recombinante (rbST), associada ou não ao FSH, sobre a população folicular, a recuperação de oócitos e a produção in vitro de embriões em vacas Gir. Utilizaram-se vacas não-lactantes, em boas condições reprodutiva e corporal, cujo ciclo estral foi sincronizado pela administração de 0,5 mg de cloprostenol. Durante o experimento, as vacas receberam continuamente implantes auriculares de norgestomet, renovados a cada 14 dias, e, posteriormente, foram submetidas aos tratamentos: T1 ou controle -punção sem tratamento pré-estimulatório; T2 -aplicação única de 160 mg de rbST antes de cada punção; e T3 -punção após o tratamento com 160 mg de rbST em aplicação única, seguida de administração de 250 UI de FSH em doses decrescentes. Os oócitos foram maturados e fertilizados in vitro e os presumíveis zigotos foram cultivados por 192 horas pós-fertilização. Os tratamentos não foram diferentes quanto à população folicular presente no ovário. Entretanto, com a administração de 250 UI de FSH (T3), verificou-se aumento no diâmetro do maior folículo e no número de folículos grandes e médios e diminuição no número de folículos pequenos. A taxa de recuperação não diferiu entre o tratamento controle e T3 (com administração de FSH), mas foi menor no T2 (aplicação, em dose única, de 150 mg de rbST antes de cada punção). O número de oócitos de grau 1 foi maior e o de degenerados, menor no T3 em relação ao T1 e ao T2. O rbST (T2) aumentou a taxa de clivagem e a produção de blastocistos e, quando associado ao FSH, melhorou a qualidade dos oócitos nos animais Gir.Palavras-chave: bovinos, Gir, punção folicular, rbST Effect of somatotropin on follicular population, oocyte retrieval and in vitro embryo production in Gir cowsABSTRACT -The aim of this study was to evaluate ovarian pre-stimulation using recombinant bovine somatotropin (rbST) or rbST associated to FSH on follicular population, oocyte retrieval and in vitro embryo production in Gir cows (Bos indicus). Estrous cycle of non-lactating cycling cows with similar body and reproductive conditions were synchronized with cloprostenol followed by norgestomet ear implants, replaced every fourteen days. The animals were randomly distributed in three treatments: TI or control (punctured without pre-stimulation), TII (injection of 160 mg of rbST, before each aspiration) and TIII (injection of 160 mg of rbST followed by administration of 250 U.I. of FSH, before each aspiration). Oocytes were maturated and fertilized in vitro and presumptive zygotes were cultured in vitro for 192 hours post-fertilization. Treatments did not differ for ovarian follicular population. However, an increase in the diameter of the largest follicle and in the number of large and medium follicles with reduction in the number of small follicles was observed for TIII. Oocyte recovery did not differ between TI and TIII, but recovery rate was lower for TII. The number of grade I oocytes was higher and the number of degenerated oocytes was lower ...

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Developmental Changes in the Expression of the Growth Hormone Receptor Messenger Ribonucleic Acid and Protein in the Bovine Ovary1

Cited by 85 publications

References 36 publications

In vitro maturation and fertilisation of bovine oocytes in relation to GH gene polymorphism (Leu/Val)

In vitro maturation and fertilisation of bovine oocytes in relation to GH gene polymorphism (Leu/Val)

Oocytes recovered from cows treated with retinol become unviable as blastocysts produced in vitro

Efeito da somatotropina na população folicular, recuperação de oócitos e produção in vitro de embriões em vacas Gir

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