Developmental changes in the expression of the GLUT2 and GLUT4 genes in the longissimus dorsi muscle of Yorkshire and Tibetan pigs

Liang, Yan; Yang, Xuemei; Gu, Yiren; Tao, Xuan; Zhong, Zhijun; Gong, Jing; Chen, X H; Lv, Xuebin

doi:10.4238/2015.february.13.7

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…Previous studies indicated that the expression level of host genes involved in glucose and amino acid metabolism or dietary intervention (low-protein and high-glucose) could affect porcine IMF deposition [31–34]. Coincidentally, we found that most of the IMF-associated bacteria identified in the cecum lumen and fecal samples have been reported to play important roles in the metabolisms of dietary nondigestible polysaccharide and amino acid.…”

Section: Discussionsupporting

confidence: 49%

16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen

Fang

Xiong

et al. 2017

BMC Microbiol

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BackgroundIntramuscular fat (IMF) that deposits among muscle fibers or within muscle cells is an important meat quality trait in pigs. Previous studies observed the effects of dietary nutrients and additives on improving the pork IMF. Gut microbiome plays an important role in host metabolism and energy harvest. Whether gut microbiota exerts effect on IMF remains unknown.ResultsIn this study, we investigated the microbial community structure of 500 samples from porcine cecum and feces using high-throughput 16S rRNA gene sequencing. We found that phylogenetic composition and potential function capacity of microbiome varied between two types of samples. Bacteria wide association study identified 119 OTUs significantly associated with IMF in the two types of samples (FDR < 0.1). Most of the IMF-associated OTUs belong to the bacteria related to polysaccharide degradation and amino acid metabolism (such as Prevotella, Treponema, Bacteroides and Clostridium). Potential function capacities related to metabolisms of carbohydrate, energy and amino acids, cell motility, and membrane transport were significantly associated with IMF content. FishTaco analysis suggested that the shifts of potential function capacities of microbiome associated with IMF might be caused by the IMF-associated microbial taxa.ConclusionsThis study firstly evaluated the contribution of gut microbiome to porcine IMF content. The results presented a potential capacity for improving IMF through modulating gut microbiota.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1055-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 49%

16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen

Fang

Xiong

et al. 2017

BMC Microbiol

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“…when analyzing the relative quantities of genes of interest related with IMF deposition (Ayuso et al, 2015;Liang et al, 2015). However, a thorough evaluation of ICGs expression stability in qPCR assay about genes related with intramuscular adipogenic differentiation has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Verification of suitable and reliable reference genes for quantitative real-time PCR during adipogenic differentiation in porcine intramuscular stromal-vascular cells

Huang

Chen

et al. 2016

Animal

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Intramuscular fat (IMF) is an important trait influencing meat quality, and intramuscular stromal-vascular cell (MSVC) differentiation is a key factor affecting IMF deposition. Quantitative real-time PCR (qPCR) is often used to screen the differentially expressed genes during differentiation of MSVCs, where proper reference genes are essential. In this study, we assessed 31 of previously reported reference genes for their expression suitability in porcine MSVCs derived form longissimus dorsi with qPCR. The expression stability of these genes was evaluated using NormFinder, geNorm and BestKeeper algorithms. NormFinder and geNorm uncovered ACTB, ALDOA and RPS18 as the most three stable genes. BestKeeper identified RPL13A, SSU72 and DAK as the most three stable genes. GAPDH was found to be the least stable gene by all of the three software packages, indicating it is not an appropriate reference gene in qPCR assay. These results might be helpful for further studies in pigs that explore the molecular mechanism underlying IMF deposition. ImplicationsThis study evaluated the expression stability of a set of well-known reference genes in porcine intramuscular stromal-vascular cells (MSVCs), whose adipogenic differentiation is critical for intramuscular fat deposition and pork quality. ACTB, ALDOA and RPS18 were identified to be most stably expressed throughout adipogenic differentiation, and suitable to be used for normalization in quantitative realtime PCR assay, whereas GAPDH was the last choice. This study has provided a valuable data for our further work to explore the molecular mechanism underlying porcine MSVCs adipogenic differentiation and intramuscular fat deposition.

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“…This is consistent with the previous study (Bosch et al 2012) who reported that IMF content increased in days 160-220 of age in muscle tissue in pigs. Moreover, the most recent data showed an increased expression of GLUT-4 and GLUT -2 in Tibetan and Yorkshire pigs during the first 6 months of ontogenesis (Liang et al 2015).…”

Section: Discussionmentioning

confidence: 97%

Expression of NR3C1, INSR and SLC2A4 genes in skeletal muscles and CBG in liver depends on age and breed of pigs

Szczepankiewicz¹,

Skrzypski²,

Pruszyńska‐Oszmałek³

et al. 2019

CZECH J ANIM SCI

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The genes encoding glucose transporter 4 (SLC2A4, GLUT4), insulin receptor (INSR) and glucocorticoid receptor (NR3C1) are considered regulators of energy metabolism that may influence fat content in skeletal muscle at different ages and breeds of pigs. In the study we performed analysis of expression of NR3C1, INSR and SLC2A4 genes in two skeletal muscle tissues: semimembranosus and longissimus dorsi muscle in gilts from three breeds of pigs that differed in intramuscular fat content: Duroc (DU), Pulawska (PUL) and Polish Large White (PLW) at 60, 90, 120, 150, 180 and 210 days after weaning. We also analyzed the expression of cortisol binding globulin (CBG) in the liver. Expression was analyzed with real time PCR (qPCR) using a relative quantification method. In blood, the concentration of cortisol, insulin and leptin were evaluated with radioimmunoassay. The concentration of metabolites (triglycerides and glucose) related to the changes of lipids content was also measured. We observed a significant relationship between the breed and the age of pigs and the expression of analyzed genes in muscle tissues (P < 0.01 for all analyzed genes) as well as with two hormones (for insulin P = 0.001; for cortisol P < 0.0001). Leptin level and metabolites in serum were not significant in the model (P > 0.05). The expression of analyzed genes (SLC2A4, INSR and NR3C1 in muscles and CBG in liver) strongly correlates with intramuscular fat content in pigs and is associated with age and breed.

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Developmental changes in the expression of the GLUT2 and GLUT4 genes in the longissimus dorsi muscle of Yorkshire and Tibetan pigs

Cited by 7 publications

References 18 publications

16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen

16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen

Verification of suitable and reliable reference genes for quantitative real-time PCR during adipogenic differentiation in porcine intramuscular stromal-vascular cells

Expression of NR3C1, INSR and SLC2A4 genes in skeletal muscles and CBG in liver depends on age and breed of pigs

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