Developmental changes in immunoreactive inhibin and FSH in plasma of chickens from hatch to sexual maturity

Vanmontfort, D; Berghman, Luc; Rombauts, Luk; Verhoeven, Guido; Decuypere, Eddy

doi:10.1080/00071669508417822

Cited by 43 publications

(19 citation statements)

References 39 publications

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“…In the present study, the inhibin α and inhibin/activin βA and βB subunits were expressed in the granulosa cells of all follicles during different stages of development from 1 to 7 weeks after hatching, indicating that granulosa cells may secrete dimeric and bioactive inhibins during the follicular development of Japanese quails. Immunolocalization of the inhibin/activin subunits in the granulosa cells of the quail ovary is in agreement with previous studies showing that the granulosa cells of chicken [18,19] and duck [30,31] ovaries are the major source of inhibin. In hens, the greatest amount of mRNA expression for the inhibin/activin βA subunit is found in the F1 follicle, while the inhibin α subunit is expressed more abundantly than the inhibin βA subunit in large preovulatory follicles.…”

Section: Sedqyar Et Al 56supporting

confidence: 79%

“…Previous studies in female chickens have shown that inhibins are involved in regulation of their reproductive function [18,19]. Ir-inhibins in plasma have been shown to increase during sexual maturation [19], and it has been reported that the ir-inhibin content of granulosa cells and secretion into culture medium is correlated with the plasma levels [32]. Yang et al [31] reported that the rise in ir-inhibin is correlated with age at sexual maturity in female ducks, while a progressive increase in steroid hormones may be consistent with a progressive increase in the steroidogenic processes of the ovary.…”

Section: Sedqyar Et Al 56mentioning

confidence: 99%

“…Inhibins selectively suppress [7][8][9][10][11][12] while activins stimulate [11][12][13][14][15] the release of FSH. Previous studies have shown that the avian ovary also produces inhibins, which plays important roles in the regulation of pituitary FSH [16][17][18][19][20][21]. However, the roles of inhibins have received little attention in quail.…”

mentioning

confidence: 99%

“…In other avian species, such as chickens [18-21, 28, 29] and ducks [30,31], similar developmental changes in circulating ir-inhibin concentrations have been observed in accordance with the ovarian activity. Previous studies in female chickens have shown that inhibins are involved in regulation of their reproductive function [18,19]. Ir-inhibins in plasma have been shown to increase during sexual maturation [19], and it has been reported that the ir-inhibin content of granulosa cells and secretion into culture medium is correlated with the plasma levels [32].…”

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confidence: 99%

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Secretion of Inhibin in Female Japanese Quails (Coturnix japonica) from Hatch to Sexual Maturity

Sedqyar

Wang

Watanabe

et al. 2008

Journal of Reproduction and Development

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Abstract.To clarify the cellular source and secretory pattern of inhibin in the Japanese quail during follicular development, the plasma concentrations of immunoreactive (ir) inhibin were measured from 1 to 7 weeks after hatching. Localization of the inhibin/activin α, βA and βB subunts was investigated by immunohistochemistry. To monitor development of the pituitary and ovarian functions, the plasma luteinizing hormone (LH) and progesterone concentrations were also measured. Ovarian weight increased gradually until 6 weeks of age and then abruptly increased at 7 weeks of age just at the onset of egg production. Plasma concentrations of LH increased significantly at 6 weeks of age. The plasma concentrations of ir-inhibin and progesterone and the pituitary contents of LH also increased significantly at 7 weeks of age. Immunohistochemically, the inhibin/activin α, βA and βB subunts were localized in the granulosa cells of all follicles during different stages of development from 1 to 7 weeks after hatching. The inhibin α, βA and βB subunts were also found in the interstitial cells but not theca cells of all follicles. These results demonstrated that the plasma concentrations of ir-inhibin of the female Japanese quails rose with ovarian development. The immunohistochemical results suggested that granulosa and interstitial cells are the major source of ovarian inhibins in female Japanese quails. Key words: Development, Female quail, Inhibin, Luteinizing hormone (LH), Progesterone (J. Reprod. Dev. 54: [52][53][54][55][56][57] 2008) nhibins and activins are structurally related dimeric gonadal proteins with the ability to regulate follicle-stimulating hormone (FSH) secretion from pituitary glands in mammals [1,2]. Inhibin consists of an α subunit linked by a disulfide bridge to one of the 2 highly homologous β subunits (βA and βB) to form inhibin A (α and βA) or inhibin B (α and βB) [3]. Apart from their action on FSH secretion, inhibins and activins have been shown to exert paracrine/autocrine effects within the gonads [4,5] and other tissues [6]. In mammalian females, the roles played by inhibin/ activin in modulating FSH secretion by the pituitary gland in vivo and in vitro have been well documented. Inhibins selectively suppress [7-12] while activins stimulate [11][12][13][14][15] the release of FSH. Previous studies have shown that the avian ovary also produces inhibins, which plays important roles in the regulation of pituitary FSH [16][17][18][19][20][21]. However, the roles of inhibins have received little attention in quail. As a laboratory animal, the female Japanese quail has been extensively used in reproductive research, because of its adaptability to battery breeding cages, small body size, early sexual maturation, short generation interval, regular egg laying and high egg production. However, studies on the reproductive endocrinology of quails are scarce. Specifically, no research results have been published concerning the secretion of inhibins in female quails. In order to clarify the possible endoc...

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Section: Sedqyar Et Al 56supporting

confidence: 79%

Section: Sedqyar Et Al 56mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Secretion of Inhibin in Female Japanese Quails (Coturnix japonica) from Hatch to Sexual Maturity

Sedqyar

Wang

Watanabe

et al. 2008

Journal of Reproduction and Development

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“…Culler and Negro-Vilar [42] reported a negative relationship between FSH and inhibin in the postnatal rat. Also, the relationship between pituitary gonadotropins and gonadal inhibin has been characterized from hatching to maturity in chickens [43,44]. Both studies found a negative relationship between inhibin and FSH in female chickens that appeared to become functional at sexual maturity.…”

Section: Discussionmentioning

confidence: 97%

Comparative Changes in the Serum Concentrations of Inhibin-B, Prolactin, Gonadotropins and Steroid Hormones at Different Reproductive States in Domestic Turkey Hens

Khalil

Hanafy

Saleh

et al. 2009

Journal of Reproduction and Development

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Abstract. The present study was undertaken to compare the changes in circulating levels of inhibin-B, prolactin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol-17β, progesterone and testosterone during the different reproductive states of turkey hens. Blood samples were collected during different reproductive states, at laying, incubating and out of lay. Inhibin-B was measured by ELISA, while other hormones were determined by Chemiluminescent Microparticle Immunoassay (CMIA). The results revealed highly significant differences among the hen's states for all serum hormone concentrations. The highest levels of inhibin-B and prolactin were observed in incubating hens, while the lowest values were observed in laying hens. In contrast, the highest levels of FSH, LH, estradiol-17β, progesterone and testosterone were found in the laying group, while the lowest values were found in the incubating group. The progesterone level was higher in the laying group compared with the other groups. These results clearly demonstrate that negative correlation was found between both the inhibin-B and prolactin levels and the gonadotropin and steroid hormone concentrations during the different reproductive states of the turkey hens. In addition, the results suggest that inhibin-B may be involved in control of FSH and LH secretion. . The existence of bioactive inhibin in testis preparations [3,4] and ovarian granulosa cells [5,6] has been reported. The effect of induction of ovarian regression and removal of ovarian follicles on plasma inhibin indicate that the ovary is the major source of inhibin in the hen [7,8]. Prolactin secretion from the avian anterior pituitary gland is principally maintained by tonic stimulation from hypothalamic prolactinreleasing factors [9]. Prolactin secretion markedly changes during the reproductive cycle of the turkey hen. The hyperprolactinemia associated with incubation behavior (broodiness) induces ovarian regression, resulting in a substantial loss of egg production in commercial breeder flocks [10]. Administration of exogenous prolactin suppresses plasma gonadotropins necessary for egg production in domestic fowl [11]. However, there is additional evidence that suppression of gonadotropin secretion in incubating birds also involves a mechanism independent of increased prolactin secretion [12,13]. In turkey hens, the changes in the concentrations of inhibin-B, prolactin, gonadotropins and progesterone have been poorly documented. Therefore, in the present study, we investigated the changes in inhibin-B, prolactin, FSH, luteinizing hormone (LH), estradiol-17β, progesterone and testosterone hormones during the different reproductive states. Materials and Methods Animals and experimental designDomestic turkey hens (local Egyptian strain) were used in the present study. The turkeys were in the first year of production. The hens received a stimulatory photoperiod of 14 h of light:10 h of dark throughout the experimental period and were maintained in floor pens with trap nests. They...

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Developmental changes in inhibin α and inhibin/activin βA and βB mRNA levels in the gonads during post‐hatch prepubertal development of male and female chickens

Onagbesan¹,

Safi²,

Decuypere³

et al. 2004

Molecular Reproduction Devel

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Dimeric inhibins and activins are barely detectable in the plasma during prepubertal development of male and female chickens. This may be misconstrued to indicate that the proteins are not produced in the gonads and have no functional significance during this period. Very few studies have actually determined the mRNA expression profile of the inhibin and activin subunits in the gonads prior to puberty in order to establish their secretion at the local level and postulate potential roles for the inhibin and activins at this developmental stage. In this study, the expression of the mRNA for the alpha-, betaA-, and betaB-subunits was determined in the ovary and testis of chickens during prepubertal development. Gene expression was determined at 3, 5, 6, 8, 10, 12, 16, and 18 weeks of age by RT-PCR. Messenger RNA level was quantified by competitive RT-PCR at 3, 6, 12, and 18 weeks of age in order to detect any changes with development, suggest potential relationship to the profile of dimeric inhibins and activins reported previously and to suggest potential paracrine and endocrine roles for them. The results show that all the inhibin/activin subunit mRNAs are expressed in the testis of the chicken throughout the period of prepubertal development up to 18 weeks of age. However, in the ovary, only the betaA- and betaB-subunits were detected at all ages whereas the alpha-subunit mRNA could only be detected just before puberty. Quantification of the mRNA levels showed variation of each subunit with age. These temporal changes suggest relationship with paracrine functional role in the ovary or the testis. Quantitative changes in expression levels also suggests that there may be some relationship between mRNA levels and the type and amount of dimeric inhibins and activins produced at any developmental stage. There are major differences between the male and female gonads in the timing of the expression of different subunits. In conclusion, the expression of the mRNA subunits in the testis and ovary suggests that inhibins and activins are being produced but may be principally involved in autocrine/paracrine function within the gonads.

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Developmental changes in immunoreactive inhibin and FSH in plasma of chickens from hatch to sexual maturity

Cited by 43 publications

References 39 publications

Secretion of Inhibin in Female Japanese Quails (Coturnix japonica) from Hatch to Sexual Maturity

Secretion of Inhibin in Female Japanese Quails (Coturnix japonica) from Hatch to Sexual Maturity

Comparative Changes in the Serum Concentrations of Inhibin-B, Prolactin, Gonadotropins and Steroid Hormones at Different Reproductive States in Domestic Turkey Hens

Developmental changes in inhibin α and inhibin/activin βA and βB mRNA levels in the gonads during post‐hatch prepubertal development of male and female chickens

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