Developmental Changes in Glycogen Content of Primordial Germ Cells in Chick Embryo.

Clawson, Robert C.; Domm, L. V.

doi:10.3181/00379727-112-28097

Cited by 34 publications

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“…In the chick, for example, primordial germ cells contain an abundance of glycogen granules in their cytoplasm during the migratory phase, but they rapidly disappear shortly after entering the gonads (Clawson and Domm, 1963). The precursor cells of osteoblasts possess an accumulation of Journal of Cell Science 118 (7) PD98059; B,E) or PI3K inhibitor (15 µM; LY294002; C,F) with 10% horse serum-DMEM for 48 hours and stained with PAS.…”

Section: Discussionmentioning

confidence: 99%

A novelSry-downstream cellular event which preserves the readily available energy source of glycogen in mouse sex differentiation

Matoba

Kanai

Kidokoro

et al. 2005

Journal of Cell Science

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Section: Discussionmentioning

confidence: 99%

A novelSry-downstream cellular event which preserves the readily available energy source of glycogen in mouse sex differentiation

Matoba

Kanai

Kidokoro

et al. 2005

Journal of Cell Science

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“…Changes in germ cell characteristics such as cytoplasmic content (Clawson and Domm, 1963), morphology (Ukeshima et al, 1991), and immunohistological properties (Urven et al, 1988;Maeda et al, 1994) have been reported during cellular migration from the vascular system to the gonadal ridge, however, the available information on the cell-biological changes in the migratory ability of germ cells is limited. In mice, testicular teratomas, or embryonic carcinoma cells, have been generated from PGCs (Stevens, 1967).…”

Section: Conservation Of Avian Genetic Resources Through Germline Chimentioning

confidence: 99%

Conservation of Avian Genetic Resources

Tajima

2013

J. Poult. Sci.

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Conservation of avian genetic resources is important, as 13.0% (1,313/10,064) of known avian species are currently categorized as endangered by the International Union for Conservation of Nature and Natural Resources (IUCN, 2012). Development of an integrated system for conservation of avian genetics is necessary; however, germplasm conservation systems developed for mammals cannot be applied directly to avians, primarily due to the unique anatomical and physiological characteristics of the avian egg. Thus an alternative strategy for conservation of oviparous species of animals must be developed. Recent technological developments for producing germline chimeras by the transfer of primordial germ cells into recipient embryos has enabled the conservation and retrieval of chicken genetic resources in their complete form. Furthermore, the possibility of propagating endangered wild birds through interspecies germline chimeras has been proposed.In the present review, the current status of avian genetic resource conservation is presented with a focus on the following 4 topics: 1) semen cryopreservation, 2) germline chimeras, 3) somatic nuclear transfer, and 4) other related technologies.

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“…Although it is known that some factors such as mouse fibroblast cells (STO) as feeder cells, stem cell factor (SCF), leukemia inhibitory factor (LIF) and basic fibroblast growth factor (bFGF ) are necessary for the proliferation and survival of mouse PGCs in vitro [10], we know little P blast of the area pellucida [2]. The cells enter the circulating blood stream, where they are called circulating PGCs (cPGCs) [3], at the first heart beats [4] and migrate to the final destination, the gonad, where they are known as gonadal PGCs (gPGCs) [5], and undergo a process of proliferation and differentiation to become two kinds of gamete, ova and spermatozoa.…”

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Survival and Proliferation of Refined Chicken Circulating Primordial Germ Cells Cultured In Vitro

Yang

Fujihara

1999

Journal of Reproduction and Development

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Abstract. In recent years, much attention has been paid to the manipulation of primordial germ cells (PGCs) to produce transgenic chicken. For this purpose, the in vitro proliferation of PGCs would make gene targeting possible, promoting the efficiency of transgenes. However, few researches have been carried out on this topic. In the present study, we tried to culture chicken PGCs outside the body. The PGCs were isolated from circulating blood of chicken embryos by the method of Ficoll density centrifugation, and labeled with PKH26 red fluorescent cell linker. The cells were refined by selection with a fine glass pipette and a micromanipulator in order to avoid the contamination of PGCs with red cells. The refined cells were then cultured for a given period. The PGCs proliferated when they were cultured on feeder cells derived from the germinal ridges of chicken embryos at stage 27. During a 5 day culture period, the highest rate of increased PGCs was about 51% for one of the two test groups. Although some kinds of growth factors were supplemented in the culture media, no synergistic effect of chicken stem cell factor (chSCF) with murine leukemia inhibitory factor (LIF) and human basic fibroblast growth factor (bFGF) on the proliferation of PGCs was found. This result implies that the LIF and bFGF influencing the proliferation of PGCs may not be conserved between mammals and birds.

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Developmental Changes in Glycogen Content of Primordial Germ Cells in Chick Embryo.

Cited by 34 publications

References 0 publications

A novelSry-downstream cellular event which preserves the readily available energy source of glycogen in mouse sex differentiation

A novelSry-downstream cellular event which preserves the readily available energy source of glycogen in mouse sex differentiation

Conservation of Avian Genetic Resources

Survival and Proliferation of Refined Chicken Circulating Primordial Germ Cells Cultured In Vitro

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