Developmental Aspects of Ultrastructure, Histochemistry and Receptivity of the Stigma of Nicotiana sylvestris

Kandasamy, Muthugapatti K.; Kristen, U.

doi:10.1093/oxfordjournals.aob.a087464

Cited by 41 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of ER, active dictyosomes and abundant starch-containing plastids in canal cells is correlated with the synthesis and secretion of proteins, polysaccharides and lipids required for pollen tube growth (Tilton and Horner, 1980;Kandasamy and Kristen, 1987;Ciampolini et al, 1990;Ciampolini and Cresti, 1997). O. sigmoideum also contains many active dictyosomes (Tilton and Horner, 1980).…”

Section: Discussionmentioning

confidence: 99%

Structure and Cytochemical Features of Stigma and Style of Ornithogalum Sigmoideum Freyn&Sint, Unpollinated and Pollinated Pistil

İsmailoğlu¹,

Ünal²

2012

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

The stigma of Ornithogalum sigmoideum is of dry and papillate type. The papillae are covered by a cuticle-pellicle layer, as revealed by staining. The activity of nonspecific esterase, acid phosphatase and peroxidase increases in the pellicle during the receptivity period. The style of O. sigmoideum is of the hollow type. Ultrastructural study of the cells lining the canal indicated that they are secretory cells and contain abundant endoplasmic reticulum, dictyosomes, mitochondria, plastids and ribosomes. After anthesis these organelles show degeneration at the end of the secretory phase. In canal cells, cytochemical tests showed the presence of acidic polyanions, insoluble and acidic polysaccharides, proteins and lipids. Before anthesis the canal cells are rich in polysaccharides, proteins and lipids. At maturity the cuticle is ruptured and secretory materials from the canal cells are released into the canal. In the unpollinated style of O. sigmoideum the exudates accumulated in the center of the canal; in pollinated pistils the same secretion materials were dispersed through the canal, which became wider. K Ke ey y w wo or rd ds s: : Ornithogalum sigmoideum, stigma, style, unpollinated style, pollinated style, cytochemistry.

show abstract

Section: Discussionmentioning

confidence: 99%

Structure and Cytochemical Features of Stigma and Style of Ornithogalum Sigmoideum Freyn&Sint, Unpollinated and Pollinated Pistil

İsmailoğlu¹,

Ünal²

2012

Acta Biologica Cracoviensia Series Botanica

View full text Add to dashboard Cite

show abstract

“…The adhesion and germination of pollen on the wet stigma is facilitated by the presence of the exudate, but its ingredients are highly variable among species Boavida et al, 2005). Tobacco (Nicotiana tabacum) is a wet stigma-type plant, and its stigma has three distinct zones: an epidermis with papillae, a subepidermal secretory zone, and a zone of parenchyma ground tissue (Kandasamy and Kristen, 1987). Elimination of the secretory zone involved in producing the exudates in tobacco by expressing a chimeric stigma-specific cytotoxic gene results in female sterility, but fertility could be restored upon application of the stigma exudate of wild-type tobacco plants (Goldman et al, 1994).…”

mentioning

confidence: 99%

Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice

et al. 2007

View full text Add to dashboard Cite

In angiosperms, the stigma provides initial nutrients and guidance cues for pollen grain germination and tube growth. However, little is known about the genes that regulate these processes in rice (Oryza sativa). Here, we generate rice stigmaspecific or -preferential gene expression profiles through comparing genome-wide expression patterns of hand-dissected, unpollinated stigma at anthesis with seven tissues, including seedling shoot, seedling root, mature anther, ovary at anthesis, seeds 5 d after pollination, 10-d-old embryo, 10-d-old endosperm, and suspension-cultured cells by using both 57 K Affymetrix rice whole-genome array and 10 K rice cDNA microarray. A high reproducibility of the microarray results was detected between the two different technology platforms. In total, we identified 548 genes to be expressed specifically or predominantly in the stigma papillar cells of rice. Real-time quantitative reverse transcription-polymerase chain reaction analysis of 34 selected genes all confirmed their stigma-specific expression. The expression of five selected genes was further validated by RNA in situ hybridization. Gene Ontology analysis shows that several auxin-signaling components, transcription, and stressrelated genes are significantly overrepresented in the rice stigma gene set. Interestingly, most of them also share several cisregulatory elements with known stress-responsive genes, supporting the notion of an overlap of genetic programs regulating pollination and stress/defense responses. We also found that genes involved in cell wall metabolism and cellular communication appear to be conserved in the stigma between rice and Arabidopsis (Arabidopsis thaliana). Our results indicate that the stigmas appear to have conserved and novel molecular functions between rice and Arabidopsis.

show abstract

“…PELP transcript levels are developmentally regulated: class II and class 111 transcripts start to appear at stages 1 and 2, respectively, and increase during flower development toward anthesis. Interestingly, the mRNA accumulation of classes II and III reaches the highest levels at about 5 to 6 days prior to anthesis, which coincides with the increase of stigma receptivity to pollen (Shivanna and Sastri, 1981;Kandasamy and Kristen, 1987). After pollination, class II and class 111 transcript levels gradually decrease, in contrast to unpollinated pistils in which they remain constant.…”

Section: Pelp Expression Profiles Suggest a Role In Reproductive Procmentioning

confidence: 88%

Developmental expression of tobacco pistil-specific genes encoding novel extensin-like proteins.

et al. 1992

View full text Add to dashboard Cite

Developmental Aspects of Ultrastructure, Histochemistry and Receptivity of the Stigma of Nicotiana sylvestris

Cited by 41 publications

References 0 publications

Structure and Cytochemical Features of Stigma and Style of Ornithogalum Sigmoideum Freyn&Sint, Unpollinated and Pollinated Pistil

Structure and Cytochemical Features of Stigma and Style of Ornithogalum Sigmoideum Freyn&Sint, Unpollinated and Pollinated Pistil

Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice

Developmental expression of tobacco pistil-specific genes encoding novel extensin-like proteins.

Contact Info

Product

Resources

About