Developmental approaches to kidney tissue engineering

Steer, Dylan; Nigám, Sanjay K.

doi:10.1152/ajprenal.00167.2003

Cited by 55 publications

(36 citation statements)

References 45 publications

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“…In an earlier work, we also provided a method for in vitro propagation of cultured embryonic kidney tissues by exploiting the power of UB branching to generate multiple recombined kidney-like tissues. 2,46 The ideal approach would be to create a kidney tissue that would elicit little to no immune response when implanted. As seen in Figure 8, the lack of an immune response allowed implanted kidney-like tissue to grow and differentiate such that extensive glomerular vasculature (apparently of host origin) was obtained.…”

Section: Discussionmentioning

confidence: 99%

Constructing Kidney-like Tissues from Cells Based on Programs for Organ Development: Toward a Method ofIn VitroTissue Engineering of the Kidney

Rosines

Johkura

Zhang

et al. 2010

Tissue Engineering Part A

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The plausibility of constructing vascularized three-dimensional (3D) kidney tissue from cells was investigated. The kidney develops from mutual inductive interactions between cells of the ureteric bud (UB), derived from the Wolffian duct (WD), and the metanephric mesenchyme (MM). We found that isolated MMs were capable of inducing branching morphogenesis of the WD (an epithelial tube) in recombination cultures; suggesting that the isolated MM retains inductive capacity for WD-derived epithelial tubule cells other than those from the UB. Hanging drop aggregates of embryonic and adult renal epithelial cells from UB and mouse inner medullary collecting duct cell (IMCD) lines, which are ultimately of WD origin, were capable of inducing MM epithelialization and tubulogenesis with apparent connections (UB cells) and collecting duct-like tubules with lumens (IMCD). This supports the view that the collecting system can be constructed from certain epithelial cells (those ultimately of WD origin) when stimulated by MM. Although the functions of the MM could not be replaced by cultured mesenchymal cells, primary MM cells and one MM-derived cell line (BSN) produced factors that stimulate UB branching morphogenesis, whereas another, rat inducible metanephric mesenchyme (RIMM-18), supported WD budding as a feeder layer. This indicates that some MM functions can be recapitulated by cells. Although engineering of a kidney-like tissue from cultured cells alone remains to be achieved, these results suggest the feasibility of such an approach following the normal developmental progression of the UB and MM. Consistent with this notion, implants of kidney-like tissues constructed in vitro from recombinations of the UB and MM survived for over 5 weeks and achieved an apparently host-derived glomerular vasculature. Lastly, we addressed the issue of optimal macro-and micro-patterning of kidney-like tissue, which might be necessary for function of an organ assembled using a tissue engineering approach. To identify suitable conditions, 3D reconstructions of HoxB7-green fluorescent protein mouse rudiments (E12) cultured on a filter or suspended in a collagen gel (type I or type IV) revealed that type IV collagen 3D culture supports the deepest tissue growth (600 AE 8 mm) and the largest kidney volume (0.22 AE 0.02 mm 3 ), and enabled the development of an umbrella-shaped collecting system such as occurs in vivo. Taken together with prior work Steer et al., 2002), 1,2 these results support the plausibility of a developmental strategy for constructing and propagating vascularized 3D kidney-like tissues from recombinations of cultured renal progenitor cells and/or primordial tissue.

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Section: Discussionmentioning

confidence: 99%

Constructing Kidney-like Tissues from Cells Based on Programs for Organ Development: Toward a Method ofIn VitroTissue Engineering of the Kidney

Rosines

Johkura

Zhang

et al. 2010

Tissue Engineering Part A

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“…Injection of stem-like cells into diseased kidneys may promote healing (20)(21)(22)(23). However, engineering a kidney-like tissue from cells with appropriate 3D spatial relationships of nephrons has yet to be achieved (24).…”

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confidence: 99%

Staged in vitro reconstitution and implantation of engineered rat kidney tissue

Rosines

Sampogna²,

Johkura³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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A major hurdle for current xenogenic-based and other approaches aimed at engineering kidney tissues is reproducing the complex three-dimensional structure of the kidney. Here, a stepwise, in vitro method of engineering rat kidney-like tissue capable of being implanted is described. Based on the fact that the stages of kidney development are separable into in vitro modules, an approach was devised that sequentially induces an epithelial tubule (the Wolffian duct) to undergo in vitro budding, followed by branching of a single isolated bud and its recombination with metanephric mesenchyme. Implantation of the recombined tissue results in apparent early vascularization. Thus, in principle, an unbranched epithelial tubular structure (potentially constructed from cultured cells) can be induced to form kidney tissue such that this in vitro engineered tissue is capable of being implanted in host rats and developing glomeruli with evidence of early vascularization. Optimization studies (of growth factor and matrix) indicate multiple suitable combinations and suggest both a most robust and a minimal system. A whole-genome microarray analysis suggested that recombined tissue recapitulated gene expression changes that occur in vivo during later stages of kidney development, and a functional assay demonstrated that the recombined tissue was capable of transport characteristic of the differentiating nephron. The approach includes several points where tissue can be propagated. The data also show how functional, 3D kidney tissue can assemble by means of interactions of independent modules separable in vitro, potentially facilitating systems-level analyses of kidney development. kidney development ͉ systems biology ͉ tissue engineering

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“…These cell aggregates already display appropriate cell signals and biomolecule secretions such that the cells organize and fuse, mimicking developing tissues [9]. This approach relies on the inbuilt programming of the cells to drive the self-organization of the tissue, meaning that an in-depth knowledge of developmental mechanisms of embryonic tissue is required [10].…”

Section: Conceptual Differencesmentioning

confidence: 99%

Bioprinting: Uncovering the Utility Layer-By-Layer

Carter¹,

Burke²,

Perriman³

2017

J. 3D Print. Med.

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ReviewBioprinting is becoming a must have capability in tissue engineering research. Key to the growth of the field is the inherent flexibility, which can be used to answer basic scientific questions that can only be addressed under 3D culture conditions, or organon-chip systems that could quickly replace underperforming animal models. Almost certainly the most challenging application of bioprinting will be for bottom-up tissue construction, which faces many of the same challenges as scaffold-based tissue engineering. In this review, the current state-of-the-art approaches to 3D bioprinting are discussed in terms of performance and suitability. This is complemented by an overview of hydrogel-based bioinks, with a special emphasis on composite biomaterial systems.

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Developmental approaches to kidney tissue engineering

Cited by 55 publications

References 45 publications

Constructing Kidney-like Tissues from Cells Based on Programs for Organ Development: Toward a Method ofIn VitroTissue Engineering of the Kidney

Constructing Kidney-like Tissues from Cells Based on Programs for Organ Development: Toward a Method ofIn VitroTissue Engineering of the Kidney

Staged in vitro reconstitution and implantation of engineered rat kidney tissue

Bioprinting: Uncovering the Utility Layer-By-Layer

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