Developmental and Ultrastructual Characteristics of Mouse Oocytes Grown in Vitro from Primordial Germ Cells

Motohashi, Hideyuki; Kada, Hidemi; Sato, Kahei

doi:10.1111/j.1749-0774.2004.tb00022.x

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Another method involves in vitro culture of follicles or ovarian tissue. Maturation of fetal primordial follicles in vitro has been reported in animals (9)(10)(11), and human fetal primordial follicles reportedly survived and secreted b-estradiol during in vitro tissue culture (12,13).…”

mentioning

confidence: 98%

Tissue culture before transplantation of frozen-thawed human fetal ovarian tissue into immunodeficient mice

Lan

Xiao

Deng

et al. 2010

Fertility and Sterility

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mentioning

confidence: 98%

Tissue culture before transplantation of frozen-thawed human fetal ovarian tissue into immunodeficient mice

Lan

Xiao

Deng

et al. 2010

Fertility and Sterility

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“…Other authors cultured whole or pieces of embryonic ovaries allowing them to attach and spread onto the bottom of the culture dish ( Figure 3c ). 27 , 31 , 32 , 33 , 34 , 35 In such cultures, mouse PGCs were able to differentiate into primary oocytes that were eventually assembled into primordial or primary follicles. Within these structures, apparently morphologically normal GV stage oocytes grew considerably in volume reaching a maximum diameter of around 65–70 μ m, but did not acquire the capability to resume and complete meiosis.…”

Section: Culture Of Reconstituted Ovariesmentioning

confidence: 99%

Complete in vitro oogenesis: retrospects and prospects

Wang

Liu

et al. 2017

Cell Death Differ

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Precise control of mammalian oogenesis has been a traditional focus of reproductive and developmental biology research. Recently, new reports have introduced the possibility of obtaining functional gametes derived in vitro from stem cells. The potential to produce functional gametes from stem cells has exciting applications for regenerative medicine though still remains challenging. In mammalian females ovulation and fertilization is a privilege reserved for a small number of oocytes. In reality the vast majority of oocytes formed from primordial germ cells (PGCs) will undergo apoptosis, or other forms of cell death. Removal occurs during germ cell cyst breakdown and the establishment of the primordial follicle (PF) pool, during the long dormancy at the PF stage, or through follicular atresia prior to reaching the ovulatory stage. A way to solve this limitation could be to produce large numbers of oocytes, in vitro, from stem cells. However, to recapitulate mammalian oogenesis and produce fertilizable oocytes in vitro is a complex process involving several different cell types, precise follicular cell–oocyte reciprocal interactions, a variety of nutrients and combinations of cytokines, and precise growth factors and hormones depending on the developmental stage. In 2016, two papers published by Morohaku et al. and Hikabe et al. reported in vitro procedures that appear to reproduce efficiently these conditions allowing for the production, completely in a dish, of a relatively large number of oocytes that are fertilizable and capable of giving rise to viable offspring in the mouse. The present article offers a critical overview of these results as well as other previous work performed mainly in mouse attempting to reproduce oogenesis completely in vitro and considers some perspectives for the potential to adapt the methods to produce functional human oocytes.

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“…Under organ culture, however, the oocytes cannot grow to more than approximately 50 µm in diameter, and the follicles cannot develop beyond the secondary follicle stage 6,7 . In order to further develop the oocytes, it is necessary to isolate preantral follicles from organ‐cultured fetal gonads 8–11 . The transplantation of female fetal gonads into immunodeficient mice leads to ovarian development in vivo .…”

Section: Introductionmentioning

confidence: 99%

Effects ofin vitroculture of mouse fetal gonads on subsequent ovarian developmentin vivoand oocyte maturationin vitro

et al. 2009

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Under organ culture, female fetal gonads in mice cannot develop beyond the preantral follicle stage unless the follicles are individually isolated and cultured again. In this study, we investigated the effect of in vitro culture of female fetal gonads before transplantation on subsequent in vivo development. The gonads derived from female fetuses 12.5 days postcoitum were organ-cultured for 0, 7 and 14 days, and then were grafted underneath the kidney capsules of severe combined immunodeficient mice and recovered at 21, 14 and 7 days post-transplantation, respectively. The histological analysis of the grafts showed that the in vitro culture of the fetal gonads restricted follicular development to the antral follicle stage post-transplantation. In the grafts cultured for 14 days, particularly, no antral follicle was observed. However, the oocytes in these follicles had grown to around 65 microm in diameter and had competence to resume meiosis in vitro. When the fetal gonads were grafted after culture for 7 and 14 days, 13.0% and 6.8% of the oocytes progressed to the metaphase II stage, respectively. These data showed significant differences (P < 0.05) in comparison with the control group (25.3%). Our results indicate that the in vitro culture of female fetal gonads before transplantation affects the subsequent in vivo development of both follicular cells and oocytes, and in vitro oocyte maturation. However, this effect seems to be more severe in terms of follicular development when compared with oocyte growth and maturation.

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Developmental and Ultrastructual Characteristics of Mouse Oocytes Grown in Vitro from Primordial Germ Cells

Cited by 5 publications

References 28 publications

Tissue culture before transplantation of frozen-thawed human fetal ovarian tissue into immunodeficient mice

Tissue culture before transplantation of frozen-thawed human fetal ovarian tissue into immunodeficient mice

Complete in vitro oogenesis: retrospects and prospects

Effects ofin vitroculture of mouse fetal gonads on subsequent ovarian developmentin vivoand oocyte maturationin vitro

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