Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11.

Mason, Ivor; Murphy, Derek; Münke, Maximilian; Francke, Uta; Elliott, Rosemary W.; Hogan, B. L. M.

doi:10.1002/j.1460-2075.1986.tb04434.x

Cited by 167 publications

(74 citation statements)

References 48 publications

(50 reference statements)

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“…This agrees with similar observations on the widespread distribution of SPARC mRNA [14] and the frequent finding that osteonectin is produced not only by bone but also by several other cells [13,[15][16][17]. The data demonstrate that this unique calciumbinding protein identified as osteonectin, SPARC or BM-40 is in fact a frequent constituent of mineralizing and non-mineralizing connective tissues and of basement membranes suggesting a rather general biological role.…”

Section: Discussionsupporting

confidence: 91%

Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC

et al. 1987

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Section: Discussionsupporting

confidence: 91%

Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC

et al. 1987

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Section: Monitoring Differentiation Of F9 and Es Cellsmentioning

confidence: 99%

“…F9 cells (Bernstine et al, 1973) were grown in DMEM ϩ 15% fetal calf serum (FCS) in flasks coated with 0.1% gelatin (all coated plastics/coverslips were BioCoat cellware; BD Biosciences, Franklin Lakes, NJ); few cells differentiate spontaneously, but differentiation was induced by adding 10 Ϫ6 M retinoic acid, 10 Ϫ4 M dibutyryl cyclical AMP, and 10 Ϫ4 M isobutylmethyl xanthine (Mason et al, 1986). A clone of ES cells (ESF 48/1) from a male 129 mouse was kindly provided by F. A. Brook and R. L. Gardner (Department of Zoology, University of Oxford, Oxford, United Kingdom).…”

Section: Cells and Buffersmentioning

confidence: 99%

“…Oct-4 (also known as Oct-3/4) is a marker for pluripotency and a member of the Pit1-Oct1-Unc86 family of transcription factors; it is required for stem cell self-renewal and is lost when ES cells differentiate (Niwa et al, 2000). Conversely, SPARC is a calcium-binding matricellular glycoprotein and a specific marker for parietal endoderm (Mason et al, 1986;Nomura et al, 1988;Yiu et al, 2001).F9 cells differentiate on addition of retinoic acid, dibutyryl cyclical AMP, and 3-isobutyl-1-methylxanthine (hereafter this treatment is called RA; Strickland and Mahdavi, 1978;Strickland et al, 1980;Gardner, 1983;Hogan et al, 1983). About 70% of the population become individual and migratory, losing Oct-4 and gaining SPARC ( Figure 1A; our unpublished data); this is typical of parietal endoderm.…”

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confidence: 99%

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A Conserved Organization of Transcription during Embryonic Stem Cell Differentiation and in Cells with High C Value

Faro-Trindade

Cook

2006

MBoC

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Although we have detailed information on the alterations occurring in steady-state levels of all cellular mRNAs during differentiation, we still know little about more global changes. Therefore, we investigated the numbers of molecules of RNA polymerase II that are active-and the way those molecules are organized-as two mouse cells (aneuploid F9 teratocarcinoma, and euploid and totipotent embryonic stem cells) differentiate into parietal endoderm. Quantitative immunoblotting shows the number of active molecules roughly halves. Transcription sites (detected by light and electron microscopy after allowing engaged polymerases to extend nascent transcripts in bromouridine-triphosphate) are uniformly distributed throughout the nucleoplasm. The numbers of such sites fall during differentiation as nuclei become smaller, but site density and diameter remain roughly constant. Similar site densities and diameters are found in salamander (amphibian) cells with 11-fold larger genomes, and in aneuploid HeLa cells. We conclude that active polymerases and their nascent transcripts are concentrated in a limited number of discrete nucleoplasmic sites or factories, and we speculate that the organization of transcription is conserved during both differentiation and evolution to a high C value. INTRODUCTIONDifferentiation involves the repression of some genes and the activation of others. For example, during erythropoiesis nuclei become smaller and heterochromatic as many genes are silenced; concurrently, globin genes become active by associating with specific nuclear sites known as "factories" or "active chromatin hubs" (Cook, 1999(Cook, , 2002de Laat and Grosveld, 2003;Osborne et al., 2004). Although microarrays provide detailed information on changes in steady-state mRNA levels during differentiation (Kelly and Rizzino, 2000;Harris and Childs, 2002;Tanaka et al., 2002;Sperger et al., 2003), there is little information on how primary rates of transcription change. For example, we do not know by how much global rates vary as any mammalian cell differentiates. Eukaryotic genomes also differ in size more than 200,000-fold in a way unrelated to organismal complexity; there is no consensus as to the basis of this C-value enigma (Hartl, 2000;Gregory, 2001), or on how transcription might be organized in such differently sized genomes.Against this background, we investigated the changes that occur in the rates and organization of transcription as two mouse cells differentiate. Mouse F9 teratocarcinoma cells are a well-studied aneuploid line that can be induced to differentiate into parietal endoderm, which is characterized by flattened cells that secrete tissue-type plasminogen activator, laminin, and type IV collagen (Strickland and Mahdavi, 1978;Strickland et al., 1980;Hogan et al., 1983). ESF/48 -1 cells are totipotent embryonic stem (ES) cells with a normal karyotype; when transferred into host blastocysts, they contribute to all tissues (including germ lines) of the resulting chimeras (Brook and Gardner, 1997), and they can be induc...

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“…Additions of growth factors or hormones to the cell cultures were made 24 h after the transfer into serum-free medium. Undifferentiated F9 teratocarcinomaderived stem cells were maintained on gelatin-coated tissue culture dishes and differentiation into parietal endoderm-like cells was initiated by addition of 5 x 10 -s M retinoic acid, 10 -~ M dibutyryl cyclic AMP and 10 -4 M isobutylmethyl xanthine, as described (Mason et al, 1986b).…”

Section: Cell Linesmentioning

confidence: 99%

Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization

Nomura¹,

Wills²,

Edwards³

et al. 1988

The Journal of Cell Biology

491

244

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Abstract. 2ar has been identified as a gene inducible by tumor promoters and growth factors in a variety of cultured mouse cell lines (Smith, J. H., and D. T. Denhardt. 1987. J. Cell. Biochem. 34:13-22 262:2900-3907.). In this paper we use Northern blot analysis and in situ hybridization to localize expression of 2ar during mouse embryogenesis. 2ar RNA is first detected in developing limb bones and calvaria at 14.5 d p.c., in a population of cells distinct from those expressing SPARC (osteonectin). High levels of 2ar expression are also seen in the bone marrow-derived granulated metrial gland cells of the deciduum and placenta, and in a number of epithelial tissues, including embryonic and postnatal kidney tubules, uterine epithelium and sensory epithelium of the embryonic ear. The temporal and spatial pattern of 2ar expression seen in vivo suggests that the protein plays a wider role than previously realized, in processes which are not confined to bone development.

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Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11.

Cited by 167 publications

References 48 publications

Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC

Solubilization of protein BM‐40 from a basement membrane tumor with cheating agents and evidence for its identity with osteonectin and SPARC

A Conserved Organization of Transcription during Embryonic Stem Cell Differentiation and in Cells with High C Value

Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization

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