Developmental and subcellular effects of chronic exposure to sub-lethal concentrations of ammonia, PAH and PCP mixtures in brown trout (Salmo trutta f. fario L.) early life stages

Luckenbach, Till; Ferling, Hermann; Gernhöfer, Maike; Köhler, Heinz‐R.; Negele, Rolf-Dieter; Pfefferle, Eva; Triebskorn, Rita

doi:10.1016/s0166-445x(03)00107-3

Cited by 21 publications

(8 citation statements)

References 45 publications

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“…However, these studies did not clearly identify the compounds and/or environmental parameters responsible for the reduced heart rate. In lab studies linked to the above mentioned field studies, for example, ammonia lead to increased heart rate in brown trout early life stages (Luckenbach et al, 2003). The results of the present experiment on the other hand revealed a direct and concentration-related effect of E2 exposure on heart rate.…”

Section: Heart Ratesupporting

confidence: 58%

Transient exposure to environmental estrogen affects embryonic development of brown trout (Salmo trutta fario)

et al. 2014

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Section: Heart Ratesupporting

confidence: 58%

Transient exposure to environmental estrogen affects embryonic development of brown trout (Salmo trutta fario)

et al. 2014

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“…It is certainly possible, however, that the concurrent effect of monogeneans, pollutants, and ammonia produced the histological damage on gills. This is supported by studies stating that hyperplasia and hypertrophy are common in cultured fish (Flores-Crespo and Flores, 2003) and fish infected by monogeneans (Thoney and Hargis, 1991;Khan and Thulin, 1991), as well as in fish exposed to polluted sediments and ammonia (Luckenbach et al, 2003;Peebua et al, 2006).…”

Section: Discussionmentioning

confidence: 83%

“…An additional potential limitation in the interpretation of the present results is that we did not record data on ammonia concentrations during the experiment. This is an important caveat since we now know about the synergistic effect of ammonia with PAHs in fish such as brown trout and tilapia (Luckenbach et al, 2003;Shailaja et al, 2006). However, this information should be interpreted carefully since only very early life stages of brown trout (eggs to 79 days old) or very young tilapia (9.3-11 g) Oreochromis mossambicus have been experimentally exposed.…”

Section: Discussionmentioning

confidence: 99%

Cichlidogyrus Sclerosus (Monogenea: Ancyrocephalinae) and Its Host, the Nile Tilapia (Oreochromis Niloticus), as Bioindicators of Chemical Pollution

Sánchez-Ramírez

Martínez

Aguirre-Macedo

et al. 2007

Journal of Parasitology

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Experimental results showed that the gill monogenean Cichlidogyrus sclerosus and its host, the Nile tilapia Oreochromis niloticus, exhibited significant numerical and physiological responses after exposure to sediments polluted with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls, and heavy metals in comparison with control fishes. After 15 days of exposure, C. sclerosus abundance significantly increased in treatments with low to fairly high sediment pollutant concentrations, but declined at high sediment pollutant concentrations. Hypertrophy and hyperplasia in secondary gill lamellae and the spleen melanomacrophage centers were significantly higher at extremely high sediment pollutant concentrations compared with the controls. Spleen lymphocyte and monocyte counts were significantly lower at extremely high sediment pollutant concentrations and were significantly correlated with high fluorescent aromatic compound concentrations measured as PAH exposure indicators. A multivariate redundancy analysis showed significant statistical association between sediment pollutant concentration, C. sclerosus abundance, and tilapia physiological variables. The polluted sediments negatively affected monogenean abundance and induced immunosuppression in hosts, consequently increasing histological damage in hosts and allowing persistent C. sclerosus infection. This study documents evidence suggesting that C. sclerosus and its host are indeed excellent models to test environmental quality in tropical freshwater ecosystems.

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“…These critical periods are thus included in worldwide directives and governmental bodies for evaluating toxicity (USEPA, ICES/IOC, OECD, OSPAR). Stressful situations caused by either genetic or external factors (physical conditions, pollutants, diet), at these sensitive stages can lead to increased disease susceptibility, reduced rate of survival, growth and reproductive success in several fish species (Sakai et al 1998;Luckenbach et al 2003), or even malformations in Senegal sole (Gavaia et al 2002). Although these changes may not be lethal, they can impair the health and/or appearance of the fish, which may result in environmental and/or aquaculture problems.…”

Section: Introductionmentioning

confidence: 99%

Variations on stress defences and metallothionein levels in the Senegal sole, Solea senegalensis, during early larval stages

Solé

Potrykus

Fernández‐Díaz

et al. 2004

Fish Physiol Biochem

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The biochemical status of antioxidant defences of larvae from the commercial, benthic fish, Solea senegalensis, were studied over a period of 28 days from hatching. The parameters studied were: catalase (EC 1.11.1.6), total glutation peroxidase activity (sum of the selenium dependent and independent forms) (EC 1.6.4.2), DT-diaphorase (EC 1.6.99.2), and as an indication of conjugative detoxifying metabolism, the enzyme glutathione S-transferase (GST; EC 2.5.1.18). Oxidative damage was evaluated by the formation of malondialdehyde (MDA) as an indication of lipid peroxidation (LP). Metallothionein (MT) levels were measured by differential pulse polarography and stress proteins (HSP 70 and HSP 60) were detected by immunoblotting, using commercial antibodies. The presence of catalytic activities was observed from hatching day and tended to increase during larval development. Significant changes were seen in most enzymes at day 3, when the larvae finished their endogenous feeding and started to feed on rotifers. DT-diaphorase activities were similar in both NADH and NADPH-dependent forms and, in turn, were about 10-times lower than their correspondent reductases. LP sharply increased at 19 and 28 days post-hatch (dph), suggesting a saturation of the antioxidant defences during metamorphosis. MT showed high values during the endogenous phase and the lowest value corresponded to day 3, when the egg-yolksac was fully reabsorbed; a steady-state value was reached thereafter. Stress proteins were present at all stages, showing distinctiveness in the molecular weight of HSP60 at hatching day and 1 dph. Quantitatively, both forms were significantly elevated at 3 dph, with their ratio (HSP70/HSP60) decreasing over time.Abbreviations: CAT -catalase; GST -glutathion S-trasferase; dph -days post hatch; DT-D -DTdiaphorase (NAD(P)H quinone oxidoreductase); DCPIP -dichlorophenol indophenol; HSP -heat shock protein; LP -lipid peroxidation; MDA -malondialdehide; MT -metallothionein; PUFA -polyunsaturated fatty acids; ROS -reactive oxygen species; t-GPX -total glutathion peroxidase.

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Developmental and subcellular effects of chronic exposure to sub-lethal concentrations of ammonia, PAH and PCP mixtures in brown trout (Salmo trutta f. fario L.) early life stages

Cited by 21 publications

References 45 publications

Transient exposure to environmental estrogen affects embryonic development of brown trout (Salmo trutta fario)

Transient exposure to environmental estrogen affects embryonic development of brown trout (Salmo trutta fario)

Cichlidogyrus Sclerosus (Monogenea: Ancyrocephalinae) and Its Host, the Nile Tilapia (Oreochromis Niloticus), as Bioindicators of Chemical Pollution

Variations on stress defences and metallothionein levels in the Senegal sole, Solea senegalensis, during early larval stages

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