Developmental and morphological studies in Japanese medaka with ultra-high resolution optical coherence tomography

Gladys, Fanny Moses; Matsuda, Masaru; Lim, Yiheng; Jackin, Boaz Jessie; Imai, Takuto; Otani, Yukitoshi; Yatagai, Toyohiko; Cense, Barry

doi:10.1364/boe.6.000297

Cited by 4 publications

(5 citation statements)

References 37 publications

(21 reference statements)

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“…Recently, the UHR-SD-OCT imaging of paralyzed medaka fish has been reported using combined SLD. 22) In conclusion, high-speed UHR OCT was demonstrated in the 800 nm wavelength region. As the broadband light source, we used a high-power Gaussian-like-shaped coherent SC with a bandwidth of 105 nm.…”

mentioning

confidence: 79%

High-speed ultrahigh-resolution spectral domain optical coherence tomography using high-power supercontinuum at 0.8 µm wavelength

Hattori

Kawagoe

Ando

et al. 2015

Appl. Phys. Express

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We demonstrated high-speed ultrahigh-resolution (UHR) optical coherence tomography (OCT) in the 800 nm wavelength region. A high-power coherent supercontinuum (SC) and a high-speed line scan camera were used to construct a spectral domain OCT. The axial resolution was 3.1 µm in air and 2.3 µm in tissue. The dependence of sensitivity on the SC power and A-scan rate was examined. For the A-scan rate of 70 kHz, the sensitivity of 104 dB was achieved for the SC power higher than 60 mW. High-speed in vivo UHR-OCT imaging was demonstrated for zebrafish embryo and swimming medaka.

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mentioning

confidence: 79%

High-speed ultrahigh-resolution spectral domain optical coherence tomography using high-power supercontinuum at 0.8 µm wavelength

Hattori

Kawagoe

Ando

et al. 2015

Appl. Phys. Express

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“…These vessel diameters corresponded to the inner diameter and were larger than our minimum diameter, with an average of 5.6 µm (7.1 ± 1.5 µm) ( Table 1). In a medaka brain, Gladys et al 28 showed only medaka's morphological features in vivo, without the delineation of the cerebral microvessels. www.nature.com/scientificreports/ To accomplish the intact in vivo high-resolution imaging of microvasculature, we established a special samplemanipulation procedure.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The high speed of the SD-OCT systems allowed not only to obtain structural tissue images and information, but also to investigate the blood flow in vivo 25 . These properties have been exploited for several studies on small aquatic animals, such as morphological studies on zebrafish 26 , 27 and medaka 28 , as well as for investigating the cardiovascular development in Xenopus 29 . In addition to morphological studies, OCT angiography (OCTA) permits the visualization of functional blood vessels in living tissues by exploiting the variation in the OCT signal caused by moving particles such as red blood cells 30 , 31 .…”

Section: Introductionmentioning

confidence: 99%

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Intact in vivo visualization of telencephalic microvasculature in medaka using optical coherence tomography

Suzuki

Ueno

Oishi

et al. 2020

Sci Rep

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To date, various human disease models in small fish—such as medaka (Oryzias lapties)—have been developed for medical and pharmacological studies. Although genetic and environmental homogeneities exist, disease progressions can show large individual differences in animal models. In this study, we established an intact in vivo angiographic approach and explored vascular networks in the telencephalon of wild-type adult medaka using the spectral-domain optical coherence tomography. Our approach, which required neither surgical operations nor labeling agents, allowed to visualize blood vessels in medaka telencephala as small as about 8 µm, that is, almost the size of the blood cells of medaka. Besides, we could show the three-dimensional microvascular distribution in the medaka telencephalon. Therefore, the intact in vivo imaging via optical coherence tomography can be used to perform follow-up studies on cerebrovascular alterations in metabolic syndrome and their associations with neurodegenerative disease models in medaka.

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“…While zebrafish have only pharyngeal teeth and lack oral teeth, medaka has both oral and pharyngeal teeth Click or tap here to enter text. Regarding shape and type, there are no differences in medaka and zebrafish as both teleosts have mild heterodonty [25,26]. Meanwhile, the development of replacement teeth in the two species includes the budding of epithelium and reciprocal interaction of the mesenchyme.…”

Section: Figure 2 Image Of Zebrafish Skeleton [25]mentioning

confidence: 99%

Exploiting the Differences Between Zebrafish and Medaka in Biological Research: A Complementary Approach

Adewoyin¹,

Teoh²,

Azmai³

et al. 2022

Pharmacophore

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Comparing two complementary species is a promising approach to broadening our understanding of disease simulation. Zebrafish and medaka are the top two fish models in biomedical research and their soaring profiles in the last three decades are compelling. Although there are far more studies using the zebrafish model than medaka in the literature, the two systems are comparable and complementary to each other. Despite the similarities, there are few anatomic and transcriptomic differences between the two species. The successful genome sequencing of medaka and zebrafish has shown that fishes and higher animals are identical in terms of genetic composition. Approximately 20,000 genes in medaka are nearly the same as that of humans with an 80% ortholog correlation while the zebrafish has a total of 26,000 genes with 71.4% of human genes. Zebrafish and medaka offer several advantages as models for investigating human disorders. Firstly, the costeffectiveness of maintaining a lab that is borne out of its small size, short generation time, and short life span is comparably better than higher animal models. Other qualities of zebrafish and medaka are high fecundity and transparent embryos which enhances visualization at different stages of embryogenesis. The purpose of this review is to highlight the anatomic and transcriptomic differences between the two species and the successes recorded so far using these teleost fishes complementarily in research, for instance in genetic manipulation. These differences which are due to evolutionary distance are the reasons why the two systems have been found complimentary.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-Share Alike 4.0 License, which allows others to remix, tweak, and build upon the work non commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Developmental and morphological studies in Japanese medaka with ultra-high resolution optical coherence tomography

Cited by 4 publications

References 37 publications

High-speed ultrahigh-resolution spectral domain optical coherence tomography using high-power supercontinuum at 0.8 µm wavelength

High-speed ultrahigh-resolution spectral domain optical coherence tomography using high-power supercontinuum at 0.8 µm wavelength

Intact in vivo visualization of telencephalic microvasculature in medaka using optical coherence tomography

Exploiting the Differences Between Zebrafish and Medaka in Biological Research: A Complementary Approach

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