Developmental and cytogenetic assessments of preimplantation embryos derived from in-vivo or in-vitro matured human oocytes

Fesahat, Farzaneh; Kalantar, Seyed Mehdi; Sheikhha, Mohammad Hasan; Saeedi, Hasan; Montazeri, Fateme; Firouzabadi, Razieh Dehghani; Khalili, Mohammad Ali

doi:10.1016/j.ejmg.2017.12.006

Cited by 14 publications

(12 citation statements)

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“…Finally, the slides were hybridized with a second set of probes. Two slides were applied for the chromosomal X series (13, 18, 21, and X) (Cytocell; Oxford Gene Technology, Oxfordshire, UK) and Y series (15,16,22, and Y) centromeric probes (Meta-Systems GmbH, Altlussheim, Germany). Then, the slides were assessed using a fluorescent microscope (Olympus BX51 and Genetics GSL-10 with BX61; Olympus, Tokyo, Japan).…”

Section: Rewashing Slides From Cleavage-stage Embryosmentioning

confidence: 99%

“…The blastocysts were graded according to the categorization system proposed by Gardner [19,20]. On day 3, the blastocyst biopsy of the surplus embryos was conducted for sex selection with assessment of the autosomal and sex chromosomes (13,15,16,18,21,22, X, and Y) via the FISH method. Blastocyst biopsy of trophectoderm (TE) cells was performed on day 5 after fertilization from hatching blastocysts [21].…”

Section: Blastocyst Grading and Biopsymentioning

confidence: 99%

“…The slides prepared from each embryo for the FISH testing for the chromosome X series (13, 18, 21, and X; Oxford Gene Technology) and Y series (15,16,22, and Y) centromeric probes (MetaSystems, Altlussheim, Germany) were checked with fluorescent microscopy. The FISH procedure was accomplished according to modified instructions (Oxford Gene Technology).…”

Section: Slide Preparations For Fishmentioning

confidence: 99%

“…Several studies have shown that multiple chromosomes (13,15,16,18,21,22, X, and Y) are involved in the high rates of aneuploidies in cleavage-stage embryos. These aneuploidies include trisomies that cause spontaneous abortion, along with those in pregnancies carried to term in the form of trisomic syndrome [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…Following such a mechanism, the aneuploid cells are excluded and do not participate in the embryo's cytogenetic status [1,2,4]. Recently, Fesahat et al [15] reported a significant difference in the aneuploidy rates between sex chromosomes and autosomal chromosomes in human cleavage-stage embryos. However, according to a previous study, biopsies are only performed at the blastocyst stage in about 7% of cycles, up from less than 1% previously [16].…”

Section: Introductionmentioning

confidence: 99%

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Reanalysis of discarded blastocysts for autosomal aneuploidy after sex selection in cleavage-stage embryos

Ebrahimian

Montazeri

Sadeghi

et al. 2020

Clin Exp Reprod Med

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Objective: The goal of the present study was to investigate the rate of chromosomal aneuploidies in surplus embryos after sex determination at the cleavage stage. Then, the same chromosomal aneuploidies were evaluated in blastocysts after extended culture.Methods: Sixty-eight surplus embryos were biopsied at the cleavage stage and incubated for an additional 3 days to allow them to reach the blastocyst stage. The embryos were reanalyzed via fluorescence in situ hybridization (FISH) to examine eight chromosomes (13, 15, 16, 18, 21, 22, X, and Y) in both cleavage-stage embryos and blastocysts.Results: Although the total abnormality rate was lower in blastocysts (32.35%) than in cleavage-stage embryos (45.58%), the difference was not significant (p=0.113). However, when we restricted the analysis to autosomal abnormalities, we observed a significant difference in the abnormality rate between the cleavage-stage embryos (44.11%) and the blastocysts (17.64%, p=0.008). A higher rate of sex chromosomal abnormalities was also observed in cleavage-stage embryos (29.4%) than in blastocysts (14.70%, p=0.038). Conclusion: The data indicated that embryo biopsy should be conducted at the blastocyst stage rather than the cleavage stage. The results also emphasized that examination of common chromosomal aneuploidies apart from sex selection cycles can be conducted in the blastocyst stage with the FISH method.

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Section: Rewashing Slides From Cleavage-stage Embryosmentioning

confidence: 99%