Developmental and communicative interrelations of Ly123 and Ly1 cell sets.

Abstract

In defining the relation ofT-cell sets to one another, the prime task is to determine, for a given pair of cell sets jointly required for a particular function, whether one set is recruited from the other by differentiation (developmental sequence) or whether the relationship is entirely communicative (collaboration or induction).The cell sets Ly123 and Lyl are a case in point. From indirect evidence it has been inferred that Ly123 is the precursor set for Lyl cells that act as helper-effectors (HE) x (Ly 1 :HE cells) (1) and for Ly 1 cells that act as suppression-inducers (SI) (Ly 1 :SI cells) (2, 3), but an entirely communicative partnership has not been ruled out.We now report direct evidence that the Lyl :HE and Lyl :SI cells are derived from the Ly123 set by differentiation, and further incidental evidence of communicative interactions involving Ly123 and Lyl cells.We obtained these data by using genetic markers (Lyt-1 allotypes of Lyt-1 congenic mice) to trace the origin of Lyl:HE and Lyl:SI cells from alternative, allotypedistinguished Ly123 cells, in the cortisone-resistant nylon-purified thymocyte (CRNPT)-macrophage (M~) test system (4) in which antigen-specific Lyl:HE (3) and Lyl:SI (3) cells are generated from CRNPT cultured on peritoneal M~ from specifically primed mice. Materials and MethodsMice. C57BL/6 (B6) mice (phenotype Lyt-l.2:Lyt-2.2) were obtained from The Jackson Laboratory, Bar Harbor, Maine. Congenie B6-Lyt-1 a mice (phenotype Lyt-1. l:Lyt-2.2) were bred at the Sloan-Kettering Institute, New York. Antigens. Preparation of group A Streptococcus vaccine (SAV) and its trinitrophenyl (TNP) conjugate (TNP-SAV) is described elsewhere (5, 6).T-M~ Culture. This procedure, in which CRNPT are cultured for 4 d on M~ from donors primed with SAV, yielding mostly Lyl cells (3), whose HE and SI functions are measured by plaque-forming cell (PFC) assay with T-depleted and intact spleen populations, respectively, is described elsewhere (3) and is briefly recapitulated in footnotes to Tables I and III. Selection of Cell Sets from CRNPT. Ly2 + cells (Ly123 and Ly23) were positively selected from B6 and B6-Lyt-1 a CRNPT as follows:Step 1: Incubation of CRNPT with ct-Lyt-2.2 (7) for 30 min on ice (2.5 × 107 CRNPT/ml of 1:50 et-Lyt-2.2 with a cytotoxicity titer of 1:640 against B6 and B6-Lyt-1" thymocytes, both B6 and B6-Lyt-1 a mice being Lyt-2.2).Step 2: Twice-washed antibody-coated cells from step 1 were applied to plastic dishes coated with immunoadsorbent-*

Quantitative studies on T cell diversity. I. Determination of the precursor frequencies for two types of streptococcus A-specific helper cells in nonimmune, polyclonally activated splenic T cells.

“…This situation is reminiscent of previous reports from our laboratory that show that most primary cytotoxic T lymphocyte (CTL) precursors are Lyt-123, whereas most Lyt-23 CTL precursors are memory cells (27,39). These results are also in line with a recent report by Shen et al (40), which revealed primary TH cells to arise primarily from the Lyt-123 T cell pool. Thus, we are presently testing the hypothesis that our frequent T. are primary TH cell precursors, whereas the rare TH are secondary TH cell precursors or memory cells.…”

Two Ly-2 T helper cell subsets distinguished by Qa-1 phenotype. The priming environment determines whether one or both subsets will be generated.

“…We have, however, examined two other functional Ly-1 subsets which, like the Ly-1 help-associated cell described here, are Qa-l+:IJ+: the Ly-1 feedback suppression inducer (FBSI) and the Ly-1 subset that induces the generation of Ly-1 :HE from Ly-i23 precursors (10,15,18,19,37). These cells are distinguished functionally by the mode in which they are assayed, their requisite cell interactions, and their precursor and inducer requirements, but have not as yet been distinguished phenotypically (1,10,15,16,18,19,(33)(34)(35)(36)(37). Whether these functional subsets involve the same or different populations capable of diverse inducer functions remains to be determined.…”

Network Regulation among T Cells: Qualitative and Quantitative Studies on Suppression in the Non‐Immune State

“…This conclusion is also in line with previous results showing that primary and secondary CTLP, when activated in bulk cultures, are Lyt-1 "*"2"' " and Lyt-1 "2"'', respectively (Simon & Abenhard 1980. Moreover, primary and secondary helper T cell precursors have been typed as Lyt-1 "''2''" and Lyt-1 "'"2", respectively (Shen et al 1980). If this conclusion were applied to the data in Figure 1 and Table I, it appears that for weak antigens such as H-Y there is a lack of memory populations, whereas for strong antigens one or 2 memory populations are present, perhaps resulting from natural exposure to cross-reactive antigens.…”