Developmental Anatomy of the Human Embryo – 3D-Imaging and Analytical Techniques

Yamada, Shigehito; Nakashima, Takashi; Hirose, Atsushi; Yoneyama, Akio; Takeda, Takeshi; Takakuwa, Tetsuya

doi:10.5772/32104

Cited by 8 publications

(8 citation statements)

References 34 publications

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“… Segment the skull from CT data: CT segmentation was conducted for each subject using a window level value of 304 Hounsfield Unit (HU) in OsiriX. Volume rending techniques were used to generate 3D reconstruction of the skull, which has shown to have enough quality to determine the 3D morphologies [ 23 ]. Identify landmarks and measure skull thickness: The pediatric head can be assumed to be symmetric [ 14 , 24 ], therefore, the landmarks were identified manually on half of the skull for each subject.…”

Section: Methodsmentioning

confidence: 99%

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A Statistical Skull Geometry Model for Children 0-3 Years Old

Park

Liu³

et al. 2015

PLoS ONE

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Head injury is the leading cause of fatality and long-term disability for children. Pediatric heads change rapidly in both size and shape during growth, especially for children under 3 years old (YO). To accurately assess the head injury risks for children, it is necessary to understand the geometry of the pediatric head and how morphologic features influence injury causation within the 0–3 YO population. In this study, head CT scans from fifty-six 0–3 YO children were used to develop a statistical model of pediatric skull geometry. Geometric features important for injury prediction, including skull size and shape, skull thickness and suture width, along with their variations among the sample population, were quantified through a series of image and statistical analyses. The size and shape of the pediatric skull change significantly with age and head circumference. The skull thickness and suture width vary with age, head circumference and location, which will have important effects on skull stiffness and injury prediction. The statistical geometry model developed in this study can provide a geometrical basis for future development of child anthropomorphic test devices and pediatric head finite element models.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Statistical Skull Geometry Model for Children 0-3 Years Old

Park

Liu³

et al. 2015

PLoS ONE

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“…[19] Recently, a high resolution three-dimensional reconstruction imaging technique called the episcopic fluorescence image capture (EIFC) was developed to image human embryos(Figure 4). [52,67,109,112] Episcopic imaging refers to imaging the surface of the 3D sample. The embryo is embedded in embedding material like paraffin and sectioned into a block.…”

Section: Imaging Human Embryosmentioning

confidence: 99%

“…The block face is imaged with successive cuts by an epifluorescence microscope, which captures the autofluorescence signal of the block surface and 2D image stacks are obtained. [52,112] These are then processed for high resolution 3D reconstructions. This technology has been used to develop the digital Human Embryo Atlas, which has proved to be a valuable source of morphological data of human embryonic development, the basis of human congenital disorders and embryonic failures.…”

Section: Imaging Human Embryosmentioning

confidence: 99%

Watching the embryo: Evolution of the microscope for the study of embryogenesis

2021

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Embryos and microscopes share a long, remarkable history and biologists have always been intrigued to watch how embryos develop under the microscope. Here we discuss the advances in microscopy which have greatly influenced our current understanding of embryogenesis. We highlight the evolution of microscopes and the optical technologies that have been instrumental in studying various developmental processes. These imaging modalities provide mechanistic insights into the dynamic cellular and molecular events which drive lineage commitment and morphogenetic changes in the developing embryo. We begin the journey with a brief history of microscopy to study embryos.First, we review the principles and optics of light, fluorescence, confocal, and electron microscopy which have been key techniques for imaging cellular and molecular events during embryonic development. Next, we discuss recent key imaging modalities such as light-sheet microscopy, which are suitable for whole embryo imaging. Further, we highlight imaging techniques like multiphoton and super resolution microscopy for beyond light diffraction limit, high resolution imaging. Lastly, we review some of the scattering-based imaging methods and techniques used for imaging human embryos.

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“…Phase-contrast X-ray imaging using a crystal X-ray interferometer (XI) (Bonse & Hart, 1965;Momose et al, 1996) has the highest sensitivity among phase-sensitive X-ray imaging methods (Yoneyama et al, 2008(Yoneyama et al, , 2015, and the density resolution of three-dimensional observation can reach 0.5 mg cm À3 (Yoneyama et al, 2013). We have studied many biological samples, for example soft tissues such as the kidney, brain and heart (Takeda et al, 2002;Yoneyama et al, 2005;Noda-Saita et al, 2006), embryos (Yamada et al, 2012), energy materials such as methane hydrates (Takeya et al, 2006), and industrial products such as lithium ion batteries (Takamatsu et al, 2018), achieved by taking advantage of the high sensitivity of XI. In addition, we developed X-ray thermography (Yoneyama et al, 2018) for detecting changes in temperature caused by thermal expansion, and we successfully performed time-resolved observation of thermal flow in heated water with a 1.3 s interval.…”

Section: Introductionmentioning

confidence: 99%

Feasibility study of interferometric phase-contrast X-ray imaging using the hard-X-ray free-electron laser of the SPring-8 Angstrom Compact Free-Electron Laser

Yoneyama¹,

Baba²,

Takamatsu³

et al. 2020

J Synchrotron Radiat

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Aiming for the fine observation of fast physical phenomena such as phonon propagation and laser ablation, phase-contrast X-ray imaging combined with a crystal X-ray interferometer and the X-ray free-electron laser (XFEL) of the SPring-8 Angstrom Compact Free-Electron Laser has been developed. An interference pattern with 70% visibility was obtained by single-shot exposure with a 15 keV monochromated XFEL. In addition, a phase map of an acrylic wedge was successfully obtained using the fringe scanning method.

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Developmental Anatomy of the Human Embryo – 3D-Imaging and Analytical Techniques

Cited by 8 publications

References 34 publications

A Statistical Skull Geometry Model for Children 0-3 Years Old

A Statistical Skull Geometry Model for Children 0-3 Years Old

Watching the embryo: Evolution of the microscope for the study of embryogenesis

Feasibility study of interferometric phase-contrast X-ray imaging using the hard-X-ray free-electron laser of the SPring-8 Angstrom Compact Free-Electron Laser

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