Developmental abnormalities and neurotoxicological effects of CuO NPs on the black sea urchin Arbacia lixula by embryotoxicity assay

Cappello, Tiziana; Catanese, Eva; Vitale, Valeria; Natalotto, Antonino; Giannetto, Alessia; Barreca, Davide; Brunelli, Elvira; Mauceri, Angela; Fasulo, Salvatore

doi:10.1016/j.marenvres.2015.05.010

Cited by 43 publications

(19 citation statements)

References 67 publications

(69 reference statements)

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“…Previous research on metal oxidative NPs found developmental toxicity induced by nano-silver ( Xin et al, 2015 ; Charehsaz et al, 2016 ; Ema et al, 2017 ; Pecoraro et al, 2017b ), nano-silica ( Yi et al, 2016 ), graphene oxide ( Buccheri et al, 2016 ; Pecoraro et al, 2017a ), zinc oxide ( Bonfanti et al, 2015 ; Zhou et al, 2015 ; Lee et al, 2016 ; Spence et al, 2016 ), iron oxide ( Di Bona et al, 2015 ), copper oxide ( Maisano et al, 2015 ; Ganesan et al, 2016 ; Torres-Duarte et al, 2016 ), magnesium oxide ( Ghobadian et al, 2015 ), titanium dioxide ( Scuderi et al, 2014 ; Parivar et al, 2015 ; Rollerova et al, 2015 ; Brundo et al, 2016 ), and cerium oxide ( Andersen et al, 2016 ). Moreover, studies have shown that bulk-Al, once entering the brain, persists for a very long time with a half-life ranging from 20% of the lifespan to greater than the entire lifespan ( Yokel, 2000 , 2002 ; Priest, 2004 ; Exley and House, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Exposure to Alumina Nanoparticles in Female Mice During Pregnancy Induces Neurodevelopmental Toxicity in the Offspring

Zhang

Ding

et al. 2018

Front. Pharmacol.

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Alumina nanoparticles (AlNP) have been shown to accumulate in organs and penetrate biological barriers which lead to toxic effects in many organ systems. However, it is not known whether AlNP exposure to female mice during pregnancy can affect the development of the central nervous system or induce neurodevelopmental toxicity in the offspring. The present study aims to examine the effect of AlNP on neurodevelopment and associated underlying mechanism. ICR strain adult female mice were randomly divided into four groups, which were treated with normal saline (control), 10 μm particle size of alumina (bulk-Al), and 50 and 13 nm AlNP during entire pregnancy period. Aluminum contents in the hippocampus of newborns were measured and neurodevelopmental behaviors were tracked in the offspring from birth to 1 month of age. Furthermore, oxidative stress and neurotransmitter levels were measured in the cerebral cortex of the adolescents. Our results showed that aluminum contents in the hippocampus of newborns in AlNP-treated groups were significantly higher than those in bulk-Al and controls. Moreover, the offspring delivered by AlNP-treated female mice displayed stunted neurodevelopmental behaviors. Finally, the offspring of AlNP-treated mice demonstrated significantly increased anxiety-like behavior with impaired learning and memory performance at 1 month of age. The underlying mechanism could be related to increased oxidative stress and decreased neurotransmitter levels in the cerebral cortex. We therefore conclude that AlNP exposure of female mice during pregnancy can induce neurodevelopmental toxicity in offspring.

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Section: Introductionmentioning

confidence: 99%

Exposure to Alumina Nanoparticles in Female Mice During Pregnancy Induces Neurodevelopmental Toxicity in the Offspring

Zhang

Ding

et al. 2018

Front. Pharmacol.

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“…Acetylcholine is regulated by the enzyme acetylcholinesterase (AChE), which is responsible for its inactivity and breakdown by hydrolysis into choline and acetate. AChE is therefore essential for the normal functioning of the branchial epithelium, and measurements of AChE activity have been widely used as a validated biomarker of neurotoxicity in aquatic bivalves (Ciacci et al 2012;D'Agata et al 2014;Cappello et al 2015;Maisano et al 2015;Natalotto et al 2015). In this study, the cholinergic neuronal system of pen shells was found not influenced by the different environmental conditions of the two sampling sites, as demonstrated by the comparable level of acetylcholine evaluated by metabolomics and the comparable enzymatic AChE activity measured in gill tissues of the pen shells from SM and FL.…”

Section: Discussionmentioning

confidence: 61%

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Cappello

Giannetto

Natalotto

et al. 2019

The European Zoological Journal

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The pen shell Pinna nobilis (Linnaeus, 1758) is the largest endemic bivalve mollusc of the Mediterranean Sea, listed as an endangered species in the European Union. Because no information is available about the adaptation of pen shells to different habitats, herein the fundamental conditions of adaptation of P. nobilis to peculiar natural environments, such as the Strait of Messina (SM) and the meromictic Faro Lake (FL; Sicily, Italy), were explored by assessing the morphology, mucous production, osmoregulation and neurotransmission of their gills. Although gills of the pen shells from both sites exhibited a regular morphology, a higher presence of acid mucous cells was detected in P. nobilis from FL than SM, as well as higher levels of osmolytes but without interfering the osmoregulatory processes. About the functioning of gills, the cholinergic (i.e. acetylcholine and AChE) neuronal system was unaltered between individuals from the two sites, whereas the GABAergic neurotransmission (i.e. 4-aminobutyrate or GABA) was significantly augmented in gills of P. nobilis from FL than SM. This may be an adaptive response to hypoxic conditions in FL, as supported by the increased hypoxiainducible factor (HIF-1α) in gills of pen shells from FL than SM. Noteworthy, this study reports for the first time the presence of the GABA neurotransmitter within the metabolite profile, obtained by application of a protonic Nuclear Magnetic Resonance (1 H NMR)-based metabolomics approach, of a marine bivalve. Therefore, GABA may be suggested as a metabolite biomarker in pen shells. Overall, findings from this study provide new insights on the behavioural and adaptive responses of the pen shell Pinna nobilis settled in different peculiar environments.

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“…A significant concentrationrelated decline in embryonic growth was observed, as well as an increase in developmental defects, including various neuronal abnormalities. It has been reported that some nanomaterials induce neuronal alteration, which suggests an NP capacity to interfere with normal neurotransmission pathways [35,38]. Recent studies have demonstrated QD-related neurotoxicity in the CNS, as well as synaptic transmission and plasticity impairments, and deteriorated brain functions in tested animals [39][40][41].…”

Section: Discussionmentioning

confidence: 99%

Potential Harm of Maltodextrin‐Coated Cadmium Sulfide Quantum Dots in Embryos and Fetuses

Reyes‐Esparza¹,

Sánchez‐Quevedo²,

Gomez-Solis³

et al. 2016

Toxicology - New Aspects to This Scientific Conundrum

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Over the past years, there has been significant interest in the study of nanoparticles for clinical applications, particularly quantum dots (QDs). However, previous studies have also shown that QDs can reach the embryo through the placenta, a natural barrier for a large variety of organic substances with diverse molecular structures, and may cause developmental deformities. Due to its essential role in a toxicological profile and its relevance to human safety, knowledge regarding embryotoxicity is of great importance. Previous studies by this research group have shown that CdS-maltodextrin QDs are biocompatible and nontoxic to cells and animals; however, QDs are able to induce embryotoxic effects. Therefore, as an effort to further address the issue, we studied the effects of CdS-maltodextrin QDs on embryo and fetus development using an embryotoxicity and teratogenicity assay on chicken embryos. Chicken embryos exposed to CdS-maltodextrin QDs (0.001, 0.01, 0.1 and 1 µg/kg) in ovo for 72 h showed growth and developmental alterations during the early stage and at the end of their development in a dose-dependent manner. Decreased development was observed during early stages (Stages 9/10 on the Hamburger-Hamilton scale) when compared with untreated eggs (Stage 13). Chicken embryos exposed to lower CdS-maltodextrin QDs doses (0.01, 0.1 and 1 ng/kg) and incubated in ovo for 21 h also showed growth and development alterations during the early stages and at the end of their development in a dosedependent manner. However, reduced development was observed at the end of the development period (21 days), and this was associated with death of the chick. Current studies have also shown that CdS-dextrin induces embryotoxicity and teratogenicity, affecting mainly the CNS, the neural tube and somites in chicken embryos. The nature of the observed abnormalities suggests that these effects could be directly associated with nanoparticle concentrations affecting somitogenesis. Therefore, according to the

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Developmental abnormalities and neurotoxicological effects of CuO NPs on the black sea urchin Arbacia lixula by embryotoxicity assay

Cited by 43 publications

References 67 publications

Exposure to Alumina Nanoparticles in Female Mice During Pregnancy Induces Neurodevelopmental Toxicity in the Offspring

Exposure to Alumina Nanoparticles in Female Mice During Pregnancy Induces Neurodevelopmental Toxicity in the Offspring

Pen shell Pinna nobilis L. (Mollusca: Bivalvia) from different peculiar environments: adaptive mechanisms of osmoregulation and neurotransmission

Potential Harm of Maltodextrin‐Coated Cadmium Sulfide Quantum Dots in Embryos and Fetuses

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