Developmental ability of chromosomally abnormal human embryos to develop to the blastocyst stage

Sandalinas, M.; Sadowy, Sasha; Alikani, Mina; Calderón, Gloria; Cohen, Jacques; Munné, Santiago

doi:10.1093/humrep/16.9.1954

Cited by 324 publications

(165 citation statements)

References 32 publications

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“…Some IVF centers even limited the last age for IVF as 43 years old [8]. Women with advanced maternal age will have poor ovary response during controlled ovary hyperstimulation (COH), low retrieved oocyte number, lowoocyte fertilization rate, low good quality embryo rate, low embryo implantation rate, low pregancy rate, high miscarriage rate, high preterm delivery rate and high birth defect rate [6,[9][10][11][12].…”

Section: Discussionmentioning

confidence: 99%

Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET)

Yan

Tang

et al. 2012

Sci. China Life Sci.

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This is a retrospective, observational study to evaluate the effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET). 11830 IVF-ET cycles from 10268 women were included. Four groups of different maternal age periods were compared. The groups were 2130 years old group (4549 cycles), 31-35 years old group (4424 cycles), 36-40 years old group (2429 cycles), and over 40 years old group (428 cycles). The mean starting dose of Gn and mean total dose of Gn in each cycle were significantly higher (P<0.01), while the mean retrieved oocyte number was significantly lower (P<0.01) in groups of higher maternal age period than those in each of the lower groups. The biochemical pregnancy rate and the clinical pregnancy rate were significantly lower (P<0.01), while the miscarriage rate was significantly higher (P<0.01) in groups of higher maternal age period than those in the lower groups. No difference was found in two-pronuclear zygotes (2PN) rate and good quality embryo rate among different groups. Birth defect rate was also comparable in the born babies in different groups. In the group with patients' age over 40 years old, the pregnancy rate was 26.87%, the clinical pregnancy rate was 19.39%, while the miscarriage rate after clinical pregnancy was 36.14%. To draw the conclusion, patients with higher maternal age had worse IVF outcomes. In women of fertile age, patients between 20 and 30 years old have the best IVF outcomes. Patients over 40 years old have poor IVF outcome and high miscarriage rate, which suggested the necessity of preimplantation genetic screening (PGS).in vitro fertilization, outcome, pregnancy rate, miscarriage rate, birth defect, maternal age Citation:Yan J H, Wu K L, Tang R, et al. Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET). Sci China Life Sci, 2012, 55: 694 -698,

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Section: Discussionmentioning

confidence: 99%

Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET)

Yan

Tang

et al. 2012

Sci. China Life Sci.

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Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

et al. 2003

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We used fluorescent in situ hybridisation (FISH) to detect nine chromosomes (1,13,15,16,17,18,21,22 and X) in 89 first Polar Bodies (1PBs), from in vitro matured oocytes discarded from IVF cycles. In 54 1PBs, we also analysed the corresponding oocyte in metaphase II (MII) to confirm the results; the other 35 1PBs were analysed alone as when preimplantation genetic diagnosis using 1PB (PGD-1PB) is performed. The frequency of aneuploid oocytes found was 47.5%; if the risk of aneuploidy for 23 chromosomes is estimated, the percentage rises to 57.2%. Missing chromosomes or chromatids found in 1PBs of 1PB/MII doublets were confirmed by MII results in 74.2%, indicating that only 25.8% of them were artefactual. Abnormalities observed in 1PBs were 55.8% whole-chromosome alterations and 44.2% chromatid anomalies. We observed a balanced predivision of chromatids for all chromosomes analysed. Differences between balanced predivision in 1PB and MII were statistically significant (Po0.0001, v 2 test); the 1PB was most affected. The mean abnormal segregation frequency for each chromosome was 0.89% (range 0.52-1.70%); so, each of the 23 chromosomes of an oocyte has a risk of 0.89% to be involved in aneuploidy. No significant differences were observed regarding age, type of abnormality (chromosome or chromatid alterations) or frequency of aneuploidy. Nine of the 35 patients (25.7%) whose 1PB and MII were studied presented abnormalities (extra chromosomes) that probably originated in early oogenesis. Analysis of 1PBs to select euploid oocytes could help patients of advanced age undergoing in vitro fertilization (IVF) treatment.

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“…While this may in part be due to the fact that the one blastomere biopsy group had a significantly higher number of embryos biopsied per PGD cycle, it is also possible that the one blastomere biopsy group contained embryos which would have been identified as mosaic unbalanced if two blastomeres had been biopsied. Sandalinas et al [11] and Emiliani et al [5] reported that mosaic embryos are capable of developing to the blastocyst stage. Based on this, it could be hypothesised that a mosaic unbalanced embryo incorrectly diagnosed as "normal/balanced" following one blastomere biopsy has the potential to reach the blastocyst stage and be transferred.…”

Section: Discussionmentioning

confidence: 99%

Preimplantation genetic diagnosis for chromosome rearrangements – one blastomere biopsy versus two blastomere biopsy

Brodie

Beyer

Osborne

et al. 2012

J Assist Reprod Genet

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Purpose Preimplantation Genetic Diagnosis (PGD) has proven to be a useful reproductive option for carriers of some chromosome rearrangements. The data presented in this study compares the impact of one versus two blastomere biopsy on the likelihood of achieving a PGD result, as well as the effect on subsequent embryo development and clinical outcomes. Methods IVF-PGD couples had either one or two blastomeres biopsied from all embryos with ≥7 blastomeres on day 3 post oocyte collection. These blastomeres were assessed for the specific chromosome rearrangement using Fluorescent In-situ Hybridisation (FISH). Further embryo development was monitored on days 4 and 5. Clinical outcomes were assessed retrospectively. Results The data shows that statistically more embryos achieved a PGD result following two blastomere biopsy, compared with one blastomere biopsy (92 % versus 88 %, respectively). Furthermore it was found that embryo development and clinical outcomes were similar between the two biopsy groups. Conclusions Based on this analysis it appears that the biopsy of two blastomeres from embryos with ≥7 blastomeres on day 3 is a valid and successful approach for couples presenting for IVF-PGD for a chromosome rearrangement.

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Developmental ability of chromosomally abnormal human embryos to develop to the blastocyst stage

Abstract: Even though there is a strong selection against chromosomally abnormal embryos, extended culture to day 5 or 6 cannot be used as a reliable tool to select against clinically relevant chromosome abnormalities such as trisomies.

Cited by 324 publications

References 32 publications

Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET)

Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET)

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

Preimplantation genetic diagnosis for chromosome rearrangements – one blastomere biopsy versus two blastomere biopsy

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