Development, validation and evaluation of an analytical method for the determination of monomeric and oligomeric procyanidins in apple extracts

Hollands, Wendy J; Voorspoels, Stefan; Jacobs, Griet; Aaby, Kjersti; Meisland, Ane; Garcı́a-Villalba, Rocío; Tomás-Barberán, Francisco A.; Piskuła, Mariusz K.; Mawson, Deborah H.; Vovk, Irena; Needs, Paul W.; Kroon, Paul A.

doi:10.1016/j.chroma.2017.03.030

Cited by 51 publications

(42 citation statements)

References 21 publications

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“…To determine the flavanol content of the apple extracts, samples were analyzed by normal phase HPLC (Agilent 1100) with separation of the analytes being achieved using a Luna Hilic column (150 × 2.0 mm; 3 µ m ) coupled with fluorescence detection as described previously. [ 16 ] Flavanol composition of the treatments is shown in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Monomeric Flavanols Are More Efficient Substrates for Gut Microbiota Conversion to Hydroxyphenyl‐γ‐Valerolactone Metabolites Than Oligomeric Procyanidins: A Randomized, Placebo‐Controlled Human Intervention Trial

Hollands

Philo

Pérez-Moral

et al. 2020

Molecular Nutrition Food Res

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Scope: The majority of ingested flavanols reach the colon where they are catabolized by the microbiota to form hydroxyphenyl--valerolactones (HGVLs). It is not known if the HGVLs are catabolic products of monomeric (epi)catechins (EPC), oligomeric procyanidins (OPCs), or both. Using data from a randomized, double-blind, placebo-controlled crossover trial the relative contributions of catechins and OPC to the bioavailable pool of HGVLs are estimated. Methods and results: Participants ingested an apple extract once daily for 28 days that delivered the following: i) 70 mg EPC and 65 mg OPC (low dose EPC), ii) 140 mg EPC and 130 mg OPC (high dose EPC), iii) 6 mg EPC and 130 mg OPC (OPC), and iv) a placebo control. Urine is collected over a 24-h period before and after treatments. The median urinary excretion of HGVLs after ingestion of the high dose EPC is tenfold higher than that excreted after ingestion of the OPC that provided an equivalent dose of PC. Approximately 22% of catechins are converted to HGVLs in contrast to PC, for which there is limited conversion. Conclusion: Monomeric catechins are efficiently converted to derived HGVLs that are absorbed and excreted in human urine, whereas oligomeric PCs are much less efficiently converted.

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Section: Methodsmentioning

confidence: 99%

Monomeric Flavanols Are More Efficient Substrates for Gut Microbiota Conversion to Hydroxyphenyl‐γ‐Valerolactone Metabolites Than Oligomeric Procyanidins: A Randomized, Placebo‐Controlled Human Intervention Trial

Hollands

Philo

Pérez-Moral

et al. 2020

Molecular Nutrition Food Res

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“…The following parameters were evaluated: selectivity, linearity (working range), precision (repeatability, intermediate precision and reproducibility), limit of detection (LOD), limit of quantification (LOQ), accuracy and robustness. All analyzes were performed in triplicate and the reliability of the parameters was verified by the relative standard deviation (RSD%) (Fernandes et al, 2015;Hollands et al, 2017). Only the procedure with complexation was validated considering that the procedure without complexation does not satisfactorily quantify the total flavonoids in A. brasiliana extract.…”

Section: Validation Of the Analytical Methodsmentioning

confidence: 99%

Development and validation of an analytical method for quantification of total flavonoids in Alternanthera brasiliana by ultraviolet-visible absorption spectrophotometry

Filho¹,

Teixeira²,

Carvalho³

et al. 2019

Afr. J. Biotechnol.

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Alternanthera brasiliana is popularly known in Brazil as "penicillin" or "benzetacil" and is used in traditional medicine for the treatment of infections and for the healing of wounds. It is also used as an ornamental plant due to the characteristic purple coloration of its leaves when cultivated in shade. The objective of this study was to develop and validate an analytical methodology by Ultraviolet-Visible absorption spectroscopy to quantify total flavonoids in crude ethanolic extract of A. brasiliana. The parameters analyzed in validation were those indicated in resolution 166/2017 of ANVISA, as selectivity/specificity, linearity, accuracy, precision, robustness, limits of detection and quantification, and also by ICH Q2(R1) for analytical validation. The method developed was simple, fast, low cost, linear, selective, precise, accuracy and robust. All parameters analyzed were within the limits recommended by the Brazilian legislation. Thus, this methodology can be useful for quality control of the extract and vegetal derivatives of A. brasiliana.

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“…HPLC analysis was performed as previously described by Holland et al with slight modifications for BLPs [47]. Samples were filtered with a 0.22 µm membrane before HPLC analysis.…”

Section: Quantification Of Pasmentioning

confidence: 99%

Mainly Dimers and Trimers of Chinese Bayberry Leaves Proanthocyanidins (BLPs) are Utilized by Gut Microbiota: In Vitro Digestion and Fermentation Coupled with Caco-2 Transportation

et al. 2020

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Chinese bayberry leaf proanthocyanidins (BLPs) are Epigallocatechin gallate (EGCG) oligomers or polymers, which have a lot of health-promoting activity. The activity is closely related to their behavior during in vitro digestion, which remains unknown and hinders further investigations. To clarify the changes of BLPs during gastrointestinal digestion, further research is required. For in vitro digestion, including gastric-intestinal digestion, colon fermentation was applied. Caco-2 monolayer transportation was also applied to investigate the behavior of different BLPs with different degrees of polymerization. The trimers and the tetramers were significantly decreased during in vitro gastric-intestinal digestion resulting in a significant increase in the content of dimers. The dimers and trimers were the main compounds utilized by gut microbiota and they were assumed not to degrade through cleavage of the inflavan bond. The monomers and dimers were able to transport through the Caco-2 monolayer at a rate of 10.45% and 6.4%, respectively.

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Development, validation and evaluation of an analytical method for the determination of monomeric and oligomeric procyanidins in apple extracts

Abstract: HighlightsMethod for simultaneous determination of individual apple procyanidins and catechins is presented.Procyanidins separated on a HILIC column.Accurate quantification achieved using isolated procyanidin oligomers.Method validated via an inter-laboratory evaluation.

Cited by 51 publications

References 21 publications

Monomeric Flavanols Are More Efficient Substrates for Gut Microbiota Conversion to Hydroxyphenyl‐γ‐Valerolactone Metabolites Than Oligomeric Procyanidins: A Randomized, Placebo‐Controlled Human Intervention Trial

Monomeric Flavanols Are More Efficient Substrates for Gut Microbiota Conversion to Hydroxyphenyl‐γ‐Valerolactone Metabolites Than Oligomeric Procyanidins: A Randomized, Placebo‐Controlled Human Intervention Trial

Development and validation of an analytical method for quantification of total flavonoids in Alternanthera brasiliana by ultraviolet-visible absorption spectrophotometry

Mainly Dimers and Trimers of Chinese Bayberry Leaves Proanthocyanidins (BLPs) are Utilized by Gut Microbiota: In Vitro Digestion and Fermentation Coupled with Caco-2 Transportation

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