Development to the Blastocyst Stage of Pig Embryos Cultured in the Amniotic Fluid of Developing Chick Embryos.

Ocampo, Marlon B.; Ocampo, Lerma C.; Suzuki, Keita; Mori, Tadashi; Ueda, Junji; Shimizu, Hiroshi; Kanagawa, Hiroshi

doi:10.1292/jvms.55.889

Cited by 6 publications

(2 citation statements)

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“…Porcine COCs were vitrified by the cryoleaf (Cooper Surgical Company, Origio, USA) method as previously described ( Somfai et al, 2014 ) with minor changes. Briefly, the COCs were initially incubated in the basic medium (BM), which was composed of modified NCSU-37 ( Ocampo et al, 1993 ) without glucose, 20 mM HEPES, 50 mM β-mercaptoethanol, 0.17 mM sodium pyruvate, 2.73 mM sodium lactate, 4 mg/ml bovine serum albumin (BSA) and 7.5 mg/ml cytochalasin B, for 30 min. The COCs were transferred into an equilibration medium (EM) for 13–15 min.…”

Section: Methodsmentioning

confidence: 99%

Glycine and Melatonin Improve Preimplantation Development of Porcine Oocytes Vitrified at the Germinal Vesicle Stage

Tang

Zhang

Liu

et al. 2022

Front. Cell Dev. Biol.

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Lipid-rich porcine oocytes are extremely sensitive to cryopreservation compared to other low-lipid oocytes. Vitrification has outperformed slowing freezing in oocyte cryopreservation and is expected to improve further by minimizing cellular osmotic and/or oxidative stresses. In this study, we compared the effects of loading porcine cumulus-oocyte complexes with glycine (an organic osmolyte) or glycine plus melatonin (an endogenous antioxidant) during vitrification, thawing and subsequent maturation to mitigate osmotic injuries or osmotic and oxidative damages on the developmental potential of porcine oocytes. Our data demonstrated that glycine treatment significantly increased the vitrification efficiency of porcine oocytes to levels comparable to those observed with glycine plus melatonin treatment. It was manifested as the thawed oocyte viability, oocyte nuclear maturation, contents of reactive oxygen species, translocation of cortical granules and apoptotic occurrence in mature oocytes, levels of ATP and transcripts of glycolytic genes in cumulus cells (markers of oocyte quality), oocyte fertilization and blastocyst development. However, the latter was more likely than the former to increase ATP contents and normal mitochondrial distribution in mature oocytes. Taken together, our results suggest that mitigating osmotic and oxidative stresses induced by vitrification and thawing can further enhance the developmental competency of vitrified porcine oocytes at the germinal vesicle stage.

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Section: Methodsmentioning

confidence: 99%

Glycine and Melatonin Improve Preimplantation Development of Porcine Oocytes Vitrified at the Germinal Vesicle Stage

Tang

Zhang

Liu

et al. 2022

Front. Cell Dev. Biol.

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“…Blakewood et al [2] and Ocampo et al [17] transferred preimplantation mammalian embryos into the amniotic cavity of developing chick embryos and observed the better growth of embryos. However, using aspirated amniotic fluid, Blakewood did not show any significant differences comparing with the control medium [2].…”

Section: -4644mentioning

confidence: 99%

Effect of Six-and Ten-Day-Old Chick Embryo Amniotic Fluid on Development of Two-Cell Mouse Embryos

Esmaili

Valojerdi

2004

Exp. Anim.

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Abstract:The purpose of this study was to investigate the effect of six-and ten-day-old chick embryo amniotic fluid (CEAF) on the development of two-cell mouse embryos. Sixand ten-day-old CEAF (6-AF, 10-AF) were aspirated separately from the amniotic cavity and two experiments were performed. In the first experiment, two-cell mouse embryos were cultured in different supplements of heat-inactivated 6-AF, pure heat-inactivated 6-AF and pure active 6-AF. The second experiment was also carried out in the same manner using the 10-AF. The rate of the development of embryos in all groups were daily determined and statistically compared with that of control (Ham's F-10 supplemented with 5mg/ml human serum albumin). During the cultivation period, more embryos reached to the blastocyst stage in all groups of CEAF compared to the control. The result hatched blastocysts embryos also increased from days 3 till 5 of cultivation in all groups of CEAF. The developmental potential of embryos appears to be almost identical in six-and ten-dayold CEAF. In conclusion, CEAF as a supplement or a natural medium could support the development of two-cell mouse embryos.

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In vitro development to blastocysts of early porcine embryos produced in vivo or in vitro

1995

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Development to the Blastocyst Stage of Pig Embryos Cultured in the Amniotic Fluid of Developing Chick Embryos.

Cited by 6 publications

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Glycine and Melatonin Improve Preimplantation Development of Porcine Oocytes Vitrified at the Germinal Vesicle Stage

Glycine and Melatonin Improve Preimplantation Development of Porcine Oocytes Vitrified at the Germinal Vesicle Stage

Effect of Six-and Ten-Day-Old Chick Embryo Amniotic Fluid on Development of Two-Cell Mouse Embryos

In vitro development to blastocysts of early porcine embryos produced in vivo or in vitro

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