Development, structure, and bioengineering of the human corneal stroma: A review of collagen-based implants

Tidu, Aurélien; Schanne-Klein, Marie-Claire; Borderie, Vincent

doi:10.1016/j.exer.2020.108256

Cited by 22 publications

(22 citation statements)

References 132 publications

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“…Decellularization of native organs allows the obtaining of biological scaffolds composed of natural extracellular matrix (ECM) that can be used in regenerative medicine for tissue and organ replacement [35]. Compared with strategies based on scaffolds generated de novo such as fibrin, collagen, or agarose applied to cornea tissue engineering [20,44], decellularization offers the possibility of obtaining a scaffold containing the Vogt palisades and crypt-like structures that are required for a proper limbal function [30]. In addition, previous works have demonstrated that the porcine limbus is structurally similar to the human limbus [45].…”

Section: Discussionmentioning

confidence: 99%

Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds

et al. 2021

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Patients with severe limbal damage and limbal stem cell deficiency are a therapeutic challenge. We evaluated four decellularization protocols applied to the full-thickness and half-thickness porcine limbus, and we used two cell types to recellularize the decellularized limbi. The results demonstrated that all protocols achieved efficient decellularization. However, the method that best preserved the transparency and composition of the limbus extracellular matrix was the use of 0.1% SDS applied to the half-thickness limbus. Recellularization with the limbal epithelial cell line SIRC and human adipose-derived mesenchymal stem cells (hADSCs) was able to generate a stratified epithelium able to express the limbal markers p63, pancytokeratin, and crystallin Z from day 7 in the case of SIRC and after 14–21 days of induction when hADSCs were used. Laminin and collagen IV expression was detected at the basal lamina of both cell types at days 14 and 21 of follow-up. Compared with control native limbi, tissues recellularized with SIRC showed adequate picrosirius red and alcian blue staining intensity, whereas limbi containing hADSCs showed normal collagen staining intensity. These preliminary results suggested that the limbal substitutes generated in this work share important similarities with the native limbus and could be potentially useful in the future.

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Section: Discussionmentioning

confidence: 99%

Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds

et al. 2021

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“…The ideal biomaterial for engineering a corneal stromal substitute needs to be transparent with adequate refractive index, strong enough to allow surgical manipulation and allow permeability of oxygen and nutrient while acting as a barrier for toxins and microorganisms. It should allow cell adhesion and migration of host epithelium and stromal cells but at the same time resist vascularization and opacification [63][64][65][66] . Natural polymers offer the advantage of biocompatibility while synthetic polymers can meet individual needs by adjusting chemical and mechanical properties 63 .…”

Section: Collagen For Engineering Corneal Stromal Constructsmentioning

confidence: 99%

“…It is compatible with cells and nerves and its ease of production explains why it is among the most studied candidates for replacing donor corneas. Rat type 1, bovine type 1, recombinant human type I and III, porcine type are all different collagen types that have been used to create collagen hydrogels, sponges or films as experimental replacements of human donor corneas 64,67 . The complex collagen organization in corneal stroma is difficult to replicate in laboratory settings and the main drawback of collagen constructs is insufficient toughness and elasticity 65 .…”

Section: Collagen For Engineering Corneal Stromal Constructsmentioning

confidence: 99%

Advanced surgeries, medicines and materials for corneal regeneration

Xeroudaki

2022

Linköping University Medical Dissertations

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“…In the long run, the failure rate of allogeneic keratoplasty will increase, and the life span of penetrating keratoplasty is usually limited to 30 years. 5 Because of the shortage of suitable corneal tissue donors, transplantation rejection, and the increased risk of disease transmission, it is difficult for regenerative therapy to obtain the expected effects and meet the growing medical needs. Therefore, to solve the above problems, there is an urgent need for substitutes of corneal tissue.…”

Section: Introductionmentioning

confidence: 99%

“…However, a study from Sweden showed that high efficacy is effective in the short term, while a 15% exclusion rate will still lead to 10% failure in 2 years. In the long run, the failure rate of allogeneic keratoplasty will increase, and the life span of penetrating keratoplasty is usually limited to 30 years . Because of the shortage of suitable corneal tissue donors, transplantation rejection, and the increased risk of disease transmission, it is difficult for regenerative therapy to obtain the expected effects and meet the growing medical needs.…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Radiation Sources on the Performance of Collagen-Based Corneal Repair Materials and Macrophage Polarization

et al. 2022

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Owing to the lack of donor corneas, there is an urgent need for suitable corneal substitutes. As the main component of the corneal stroma, collagen has great advantages as a corneal repair material. If there are microorganisms such as bacteria in the corneal repair material, it may induce postoperative infection, causing the failure of corneal transplantation. Therefore, irradiation, as a common sterilization method, is often used to control the microorganisms in the material. However, it has not been reported which type of radiation source and what doses can sterilize more effectively without affecting the properties of collagen-based corneal repair materials (CCRMs) and have a positive impact on macrophage polarization. In this study, three different radiation sources of ultraviolet, cobalt-60, and electron beam at four different doses of 2, 5, 8, and 10 kGy were used to irradiate CCRMs. The swelling, stretching, transmittance, and degradation of the irradiated CCRMs were characterized, and the proliferation of human corneal epithelial cells on the irradiated CCRMs was characterized using the CCK8 kit. The results showed that low dose (<5 kGy) of radiation had little effect on the performance of CCRMs. Three irradiation methods with less influence were selected for the further study on RAW264.7 macrophage polarization. The results indicated that CCRMs treated with UV could downregulate the expression of pro-inflammatory related genes and upregulate the expression of anti-inflammatory genes in macrophages, which indicated that UV irradiation is a beneficial process for the preparation of CCRMs.

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Development, structure, and bioengineering of the human corneal stroma: A review of collagen-based implants

Cited by 22 publications

References 132 publications

Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds

Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds

Advanced surgeries, medicines and materials for corneal regeneration

Effects of Different Radiation Sources on the Performance of Collagen-Based Corneal Repair Materials and Macrophage Polarization

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