Development of Whitening Cosmetic Ingredients from Cudrania tricuspidata Stem Extract

Abstract

This study was conducted to investigate the possibility of Cudrania tricuspidata (C. tricuspidata) stem extract as whitening cosmetic material. Methods: We performed TLC and HPLC analysis to investigate the anti-oxidant compounds and its contents in C. tricuspidata stem extract fractions (hexane, ethyl acetate, butanol, and water). In order to evaluate anti-oxidant effects, radical and superoxide scavenging activities were conducted by using DPPH, ABTS free radicals and xanthine oxidases, respectively. In addition, we examined tyrosinase activity and cellular melanin content to verify the whitening effects of C. tricuspidata stem extract. Results: We found out that both ethyl acetate fraction and butanol fraction among the fractions of C. tricuspidata stem extract had anti-oxidant compounds. Our results pointed out that the active fractions have significant free radical and superoxide scavenging activities, and the fraction of ethyl acetate is the best. The ethyl acetate fraction (IC 50 24.4 ppm) and butanol fraction (IC 50 88.3 ppm) reduced tyrosinase activities and melanin content in a concentration-dependent manner. Conclusion: Through the results, C. tricuspidata stem extract has the potent as a cosmetic material which possess the anti-oxidant and whitening effects.

Cudrania tricuspidata: an updated review on ethnomedicine, phytochemistry and pharmacology

“…Accumulated studies have revealed that the extracts and components of C. tricuspidata exhibited a broad spectrum of pharmacological activities, including anti-inammatory, 22,23 antioxidant, 78,79 antitumor, 16,24 hepatoprotective, 25,26 neuroprotective, 27,28 antiobesity, 18,19 immunomodulatory, 70,71 antiatherosclerotic, 80,81 antimicrobial, 11,76 skin-protecting, 79,82 and antidiabetic 21,52 effects. The presence of a variety of bioactive compounds may be synergistically or individually responsible for the various activities of this species (Fig.…”

“…82 The EtOAc fraction (IC 50 ¼ 24.4 ppm) and the n-BuOH fraction (IC 50 ¼ 88.3 ppm) of the C. tricuspidata stem extract could reduce the activity of tyrosinase and the melanin content in a concentrationdependent manner. 79 It was found that the avonoids steppogenin (149, IC 50 ¼ 2.52 mM) and trans-dihydromorin (160, IC 50 ¼ 21.54 mM) and the phenylpropanoid oxyresveratrol (IC 50 ¼ 2.85 mM) from the twigs of C. tricuspidata displayed potent inhibitory activities against mushroom tyrosinase and the melanogenesis process in melanocytes, which suggested their potential to be developed as skin-whitening agents in cosmetics and antibrowning agents in food. 75 The tyrosinase-inhibiting activity of the avonoids could be affected by the hydroxyl groups substituted at the 2-and 4-positions of the aromatic ring.…”

Optimization of Jirisan Mountain Cudrania tricuspidata leaf substance extraction across solvents and temperatures

“…Cudrania tricuspidata has traditionally been used as a medicinal plant for conditions including eczema, tuberculosis, and chronic back pain [ 1 ]. According to previous studies, the plant has many beneficial characteristics, including antioxidative [ 2 ], antimicrobial [ 3 ], antitumor [ 4 ], antiobesity [ 5 ], anti-osteoarthritis [ 6 ], antiaging [ 7 ], antiinflammatory, and neuroprotective [ 8 ] properties. It also shows efficacy against cardiovascular diseases [ 9 ] such as atherosclerosis and hypertension [ 10 ].…”

Biological Activity Study on Anti-oxidant, Whitening, and Anti-inflammatory Effects of Astragalus membranaceus Ethanol Extracts and Bioconversion Extracts

“…최근 천연 소재를 활용한 기능성 화장품의 소재 개발 연구가 진행되고 있으며 (Ha & You, 2016;Han et al, 2016;Yu & Kim, 2017) (Lim et al, 2003 Tween 20, pH 7.5)]에 희석한 β-actin primary antibody…”