Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene

Chen, W. P.; Chen, P. D.; Liu, D. J.; Kynast, Ralf G.; Friebe, Bernd; Velazhahan, R.; Muthukrishnan, Subbaratnam; Gill, Bikram S.

doi:10.1007/s001220051294

Cited by 174 publications

(82 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results clearly showed a lack of correlation between herbicide resistance and disease resistance except for lines 4 and 15, in which all phenotypes were expressed. Previous reports have shown that TLP expression in rice and wheat significantly reduced development of Rhizoctonia solani and Fusarium graminearum, respectively (Chen et al 1999;Datta et al 1999). Our results also expand on a previous report of enhanced resistance in transgenic carrot due to heterologous expression of chitinases (Punja and Raharjo 1996) and demonstrate the potential utility of herbicide resistance in carrot derived through genetic engineering.…”

Section: Resistance Of Transgenic Plants To the Herbicide Libertysupporting

confidence: 87%

“…Among the PR proteins, thaumatinlike proteins (TLP), belonging to the PR-5 group, when expressed in transgenic rice and wheat, successfully reduced development of disease symptoms caused by Fusarium and Rhizoctonia (Chen et al 1999;Datta et al 1999). TLPs are reported to exert antifungal activity by altering plasma membrane permeability, affecting signal transduction cascades, or hydrolysing polymeric β-1, 3-glucans in the fungal cell wall (Koiwa et al 1997;Grenier et al 1999).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transgenic herbicide- and disease-tolerant carrot (Daucus carota L.) plants obtained through Agrobacterium-mediated transformation

Chen

Punja

2002

Plant Cell Rep

View full text Add to dashboard Cite

Transgenic carrot (Daucus carota L.) plants expressing a rice thaumatin-like protein (tlp), phosphinothricin acetyltransferase (bar) and the hygromycin phosphotransferase (hpt) genes were obtained by Agrobacterium-mediated transformation. Petiole and hypocotyl segments of three carrot cultivars were used as the explant sources. Following infection, selection was achieved on Murashige and Skoog medium with 1 mg/l phosphinothricin or 25 mg/l hygromycin B, which was increased after 2 weeks to 10 mg/l phosphinothricin and 100 mg/l hygromycin B. The presence of the tlp and bar transgenes was confirmed by polymerase chain reaction and Southern blot analyses, and the expression of the thaumatin-like protein was demonstrated by Western blot analysis. Among 45 primary transformants, 13 were selected for assessment of herbicide and/or disease tolerance. The transgenic plants showed varying levels of tolerance to the herbicide phosphinothricin, depending on the transformation events in different lines. Four transgenic lines also showed significantly enhanced tolerance to the foliar and root pathogen Botrytis cinerea or Sclerotinia sclerotiorum when inoculated under controlled environment conditions. Two lines had significantly enhanced tolerance to the herbicide phosphinothricin as well as to both pathogens. These results demonstrate the feasibility of introducing two potentially useful agronomic traits into carrot through genetic engineering.

show abstract

Section: Resistance Of Transgenic Plants To the Herbicide Libertysupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Transgenic herbicide- and disease-tolerant carrot (Daucus carota L.) plants obtained through Agrobacterium-mediated transformation

Chen

Punja

2002

Plant Cell Rep

View full text Add to dashboard Cite

show abstract

“…Pritsch et al (2000) observed greater and earlier induction of PR-5 transcripts in resistant wheat cultivar (Sumai 3), compared with susceptible cultivar (Wheaton) when inoculated with Fusarium graminearum, the head blight pathogen. It has been demonstrated that overexpression of chitinase, b-1,3-glucanse and TLP in transgenic plants was associated with enhanced resistance to various fungal pathogens (Broglie et al 1991;Lin et al 1995;Chen et al 1999;Datta et al 1999;Anand et al 2003;Velazhahan and Muthukrishnan 2004;Kalpana et al 2006). The results of the present study indicated that the accumulation of chitinase was more pronounced in rice plants sprayed with OA.…”

Section: Discussionsupporting

confidence: 53%

Oxalic acid-induced resistance toRhizoctonia solaniin rice is associated with induction of phenolics, peroxidase and pathogenesis-related proteins

Jayaraman

Bhuvaneswari

Rabindran

et al. 2010

Journal of Plant Interactions

View full text Add to dashboard Cite

Oxalic acid (1 mM) when applied as a foliar spray to rice plants induced resistance to challenge infection with Rhizoctonia solani, the rice sheath blight pathogen. Maximum reduction in sheath blight incidence was observed when the plants were sprayed with oxalic acid three days before inoculation with the fungus. The biochemical alterations in rice plants treated with oxalic acid was also investigated. When rice plants were treated with oxalic acid, a two-fold increase in phenolic content in leaf sheaths was recorded three days after treatment. Phenylalanine ammonia-lyase and peroxidase activities increased significantly starting from two days after treatment. Peroxidase (PO) isozyme analysis indicated that PO-3 and PO-4 were induced two days after treatment with oxalic acid. Western blot analysis revealed that two chitinases (28 and 35 kDa) and two b-1,3-glucanases (30 and 32 kDa) were strongly induced in rice sheaths four to six days after treatment with oxalic acid. Immunoblot analysis of protein extracts from oxalic acid-treated plants demonstrated the induction of a 23 kDa thaumatinlike protein (TLP) cross-reacting with bean TLP antibody. These results suggest that the enhanced activities of defense enzymes and defense-related compounds in oxalic acid-treated rice plants may contribute to resistance against R. solani.

show abstract

“…In addition to inhibiting hyphal growth and spore germination, some TLPs greatly increase the permeability of fungal and oomycete plasma membranes (Yun et al 1997;Kitajima and Sato 1999). Overexpression of TLPs appears to enhance resistance to fungal disease (Liu et al 1994;Zhu et al 1996;Chen et al 1999). Moreover, TLPs in various plant-derived foods (Hoffmann-Sommergruber 2002) and conifer pollen (Midoro-Horiuti et al 2000;Cortegano et al 2004) are allergens.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Thaumatin-like Gene Family and Identification of Pectobacterium carotovorum subsp. carotovorum Inducible Genes in Brassica oleracea

Ahmed¹,

Park²,

Jung³

et al. 2013

Plant Breed. Biotech.

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Enhancement of resistance by exploiting stress resistance-related genes offers the most efficient approach to address this issue. Among the stress resistance-related genes, thaumatin-like proteins (TLPs) play a vital role in enhancement of resistance against stresses. In this study, we identified 12 TLPs from B. oleracea genomic DNA sequencing database, analyzed their sequences and compared with other published pathogenesis-related TLPs, and found a high degree of homology with them. In addition, these genes showed an organ-specific expression, three of which expressed differentially after Pectobacterium carotovorum subsp. carotovorum infection in cabbage plants. Data obtained in this study suggest the probable involvement of TLPs in resistance against soft rot disease of Brassica.

show abstract

Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene

Cited by 174 publications

References 30 publications

Transgenic herbicide- and disease-tolerant carrot (Daucus carota L.) plants obtained through Agrobacterium-mediated transformation

Transgenic herbicide- and disease-tolerant carrot (Daucus carota L.) plants obtained through Agrobacterium-mediated transformation

Oxalic acid-induced resistance toRhizoctonia solaniin rice is associated with induction of phenolics, peroxidase and pathogenesis-related proteins

Characterization of Thaumatin-like Gene Family and Identification of Pectobacterium carotovorum subsp. carotovorum Inducible Genes in Brassica oleracea

Contact Info

Product

Resources

About