Development of visualization and analysis methods for evaluating intratumoral nanoparticle kinetics for tumor-targeted drug delivery using Förster resonance energy transfer in vivo live imaging and tissue clearing techniques

Togami, Kohei; Ishizawa, Kiyomi; Yasuda, Mio; Tada, Hitoshi; Chono, Sumio

doi:10.1016/j.jpba.2022.115127

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Lectin dyes bind to the luminal side of endothelial cells and have been applied to image the architecture of the vascular network in mouse models of glioma using 3DISCO ( 49 ) and FluoClearBABB ( 50 ), HNSCC xenografts from human cell lines using the commercial Binaree clearing kit ( 51 ), human hepatoma xenografts ( 52 ) and human pancreatic ductal adenocarcinoma (PDAC) xenografts in mice cleared with CUBIC ( 53 ), and a genetic PDAC mouse model cleared with FLASH ( 11 ). Lectins have also been used to label the vasculature of entire mice with primary and metastatic orthotopic tumor models for breast cancer, PDAC, and lung cancer cleared with vDISCO ( 48 ).…”

Section: Applying Tissue Clearing To Inspect Tumors and Their Tme In 3dmentioning

confidence: 99%

Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time

Almagro

Messal

2023

Front. Immunol.

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Volume imaging visualizes the three-dimensional (3D) complexity of tumors to unravel the dynamic crosstalk between cancer cells and the heterogeneous landscape of the tumor microenvironment (TME). Tissue clearing and intravital microscopy (IVM) constitute rapidly progressing technologies to study the architectural context of such interactions. Tissue clearing enables high-resolution imaging of large samples, allowing for the characterization of entire tumors and even organs and organisms with tumors. With IVM, the dynamic engagement between cancer cells and the TME can be visualized in 3D over time, allowing for acquisition of 4D data. Together, tissue clearing and IVM have been critical in the examination of cancer-TME interactions and have drastically advanced our knowledge in fundamental cancer research and clinical oncology. This review provides an overview of the current technical repertoire of fluorescence volume imaging technologies to study cancer and the TME, and discusses how their recent applications have been utilized to advance our fundamental understanding of tumor architecture, stromal and immune infiltration, vascularization and innervation, and to explore avenues for immunotherapy and optimized chemotherapy delivery.

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Section: Applying Tissue Clearing To Inspect Tumors and Their Tme In 3dmentioning

confidence: 99%

Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and time

Almagro

Messal

2023

Front. Immunol.

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“…In combination with tissue clearing, Forster resonance energy transfer (FRET) imaging can elucidate the release kinetics and degradation mechanism of nanoparticles in the complex tumor microenvironment. 126 Dynamic positron emission tomography (PET) imaging can be used to obtain the distribution of a reported nanotube abbreviated as SWNCT in the renal and coronary arteries based on functional metabolism. 127 Furthermore, the ultrasound imaging system, which can achieve super-resolution and real-time dynamic imaging, is highly safe and radiation-free.…”

Section: Particles Distribution In Tumorsmentioning

confidence: 99%

“…It is used to explore the targeting efficiency of therapeutic antibodies against cancer metastases with a uniform imaging resolution. In combination with tissue clearing, Forster resonance energy transfer (FRET) imaging can elucidate the release kinetics and degradation mechanism of nanoparticles in the complex tumor microenvironment …”

Section: Evaluation Of Structure-oriented Ddssmentioning

confidence: 99%

Advances in Structure Pharmaceutics from Discovery to Evaluation and Design

Xue

et al. 2023

Mol. Pharmaceutics

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Drug delivery systems (DDSs) play an important role in delivering active pharmaceutical ingredients (APIs) to targeted sites with a predesigned release pattern. The chemical and biological properties of APIs and excipients have been extensively studied for their contribution to DDS quality and effectiveness; however, the structural characteristics of DDSs have not been adequately explored. Structure pharmaceutics involves the study of the structure of DDSs, especially the three-dimensional (3D) structures, and its interaction with the physiological and pathological structure of organisms, possibly influencing their release kinetics and targeting abilities. A systematic overview of the structures of a variety of dosage forms, such as tablets, granules, pellets, microspheres, powders, and nanoparticles, is presented. Moreover, the influence of structures on the release and targeting capability of DDSs has also been discussed, especially the in vitro and in vivo release correlation and the structure-based organ- and tumor-targeting capabilities of particles with different structures. Additionally, an in-depth discussion is provided regarding the application of structural strategies in the DDSs design and evaluation. Furthermore, some of the most frequently used characterization techniques in structure pharmaceutics are briefly described along with their potential future applications.

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