Development of Virus Concentration Method Using Negatively Charged Membrane by Alkaline Elution after Acid Rinse.

Katayama, Hiroyuki; Shimasaki, Akihiro; Ohgaki, Shinichiro

doi:10.2965/jswe.25.469

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…The probe and primers used were as reported previously. 26 PCR conditions were as follows: 30 min at 48 • C for cDNA synthesis, 3 min at 95 • C for pre-heating, 20 s at 95 • C, 30 s at 55 • C and 40 cycles for 45 s at 72 • C.…”

Section: Qpcrmentioning

confidence: 99%

Photocatalytic inactivation of bacteriophages by TiO2-coated glass plates under low-intensity, long-wavelength UV irradiation

Ishiguro

Nakano

Yao

et al. 2011

Photochem Photobiol Sci

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The International Organization for Standardization (ISO) was used to evaluate antibacterial activity by titanium dioxide (TiO(2)) photocatalysis since 2006. We evaluated photocatalytic inactivation of Qβ and T4 bacteriophages induced by low-intensity, long-wavelength ultraviolet A (UVA; 0.1 mW cm(-2) and 0.001 mW cm(-2)) irradiation on a TiO(2)-coated glass plate using the ISO methodology. The results indicated that both bacteriophages were inactivated at 0.001 mW cm(-2) UVA. The intensity of UV light, including long-wavelength light (UVA), is very low in an actual indoor environment. Thus, TiO(2) photocatalysis can be beneficial for inactivating viruses in an indoor environment. Experiments using qPCR and bovine serum albumin degradation assume that viral inactivation is caused by outer viral protein disorder and not by viral RNA reduction by reactive oxygen species produced during TiO(2) photocatalysis. Furthermore, we showed that the ISO methodology for standard testing of antibacterial activity by TiO(2) photocatalysis can be applied to assess antiviral activity.

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Section: Qpcrmentioning

confidence: 99%

Photocatalytic inactivation of bacteriophages by TiO2-coated glass plates under low-intensity, long-wavelength UV irradiation

Ishiguro

Nakano

Yao

et al. 2011

Photochem Photobiol Sci

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“…Amplification was conducted at 95°C for 30 sec and 50 cycles of 95°C for 5 sec and 60°C for 30 sec in Thermal Cycler Dice Model TP800. One set of primers, including forward and reverse primers (5-TCA AGC CGT GAT AGT CGT TCC TC-3 and 5-AAT CGT TGG CAA TGG AAA GTG C-3 starting at base 49 and 187, respectively), and the probe (5 FAM-CGA GCC GCG AAC ACA AGA ATT GA-3 TAMRA starting at base 147) were used in the quantification of GIII F-RNA bacteriophages (Katayama et al, 2002b). Target templates of the primer set was validated using Primer-BLAST software (Ye et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Fecal Indicators in Channels of Johkasou Systems

Setiyawan

Yamada

Fajri

et al. 2014

J. of Wat. ^|^ Envir. Tech.

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A johkasou system is getting popular as a decentralized wastewater treatment system in low-density communities. Treated waters of the johkasou systems, which are generally discharged into channels within residential areas before entering the local receiving water body, may contain microbial contaminants. To identify the characteristics of fecal indicators in the channels of the johkasou systems, the concentrations of fecal indicators (F-RNA bacteriophages, GIII F-RNA bacteriophages, Escherichia coli, and total coliforms) were examined both in water and sediment in the channels of three johkasou systems. These fecal indicators were always detected at high levels both in water and sediment in the channels of three johkasou systems through the study period. Significant differences in concentrations of fecal indicators were not observed among three johkasou systems in different residential areas. Concentrations of fecal indicators fluctuated in a day mainly due to domestic activities. Significant positive correlations of total coliforms and E. coli in water with total coliforms and E. coli in sediment suggest the potential importance of sediment on microbial dynamics in the channels of the johkasou systems. No significant correlations of F-RNA bacteriophages with total coliforms and E. coli indicate a specific distribution mechanism for F-RNA bacteriophages.

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“…Adsorption-elution methods are widely accepted as virus concentration methods; however, their elution efficiencies occasionally become low, even though their adsorption efficiencies are high (27,41,42). Additionally, it was suggested that FRNAPH Q␤ is inactivated before the elution step when an electronegative membrane is used (26). Thus, in our methods, the membranes were subjected to FRNAPH propagation immediately after the adsorption or entrapment step.…”

Section: Discussionmentioning

confidence: 99%

“…To determine an appropriate method for recovery and quantitative genotyping of infectious FRNAPHs in the water environment, two types of FRNAPH recovery methods, an adsorption method using an electronegative MF and an entrapment method using a noncharged MF, were compared. For their validation, Q␤ was preferentially used because it was presumed that Q␤ is easily inactivated during the processes (26). The entrapment method, which showed better recovery, was further validated and was applied to surface water samples.…”

Section: Frnaph Strainsmentioning

confidence: 99%

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Quantitative Distribution of Infectious F-Specific RNA Phage Genotypes in Surface Waters

Hata

Hanamoto

Shirasaka

et al. 2016

Appl Environ Microbiol

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F-specific RNA phages (FRNAPHs) are considered potential viral indicators of water pollution due to their occurrence and stability in water environments. However, their suitability as viral indicators is not fully elucidated because the characteristics of FRNAPHs are variable depending on the genotype. In this study, for the characterization of infectious FRNAPH genotypes, integrated culture reverse transcription-PCR coupled with the most probable number approach was applied to surface water samples. Further, to recover low concentrations of FRNAPH genotypes, an FRNAPH recovery method was developed. The novel FRNAPH recovery method using a noncharged microfiltration membrane could effectively recover FRNAPH strains without inactivation, while a method using an electronegative microfiltration membrane resulted in the inactivation of some strains. Infectious FRNAPH genotypes in surface water samples were successfully quantified with an efficiency comparable to that of the conventional plaque assay. Genotype I (GI) and GII FRNAPHs tended to be predominant at locations impacted by treated and untreated municipal wastewater, respectively. The numbers and proportions of infectious FRNAPHs tended to be higher during the winter season when water temperature decreased. IMPORTANCE Properties of FRNAPHs are highly variable depending on their genotypes. Previous typing methods for

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Development of Virus Concentration Method Using Negatively Charged Membrane by Alkaline Elution after Acid Rinse.

Cited by 11 publications

References 12 publications

Photocatalytic inactivation of bacteriophages by TiO2-coated glass plates under low-intensity, long-wavelength UV irradiation

Photocatalytic inactivation of bacteriophages by TiO2-coated glass plates under low-intensity, long-wavelength UV irradiation

Characteristics of Fecal Indicators in Channels of Johkasou Systems

Quantitative Distribution of Infectious F-Specific RNA Phage Genotypes in Surface Waters

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