Development of Virtual Histology and Virtual Biopsy Using Laser-scanning Confocal Microscopy

Inoue, Haruhiro; Cho, J Y; Satodate, Hitoshi; Sakashita, Masanori; Hidaka, Eiji; Fukami, Shinjiro; Kazawa, Tamae; Yoshida, Takeshi; Shiokawa, Akira; Kudo, Shin Ei

doi:10.1080/00855910310001485

Cited by 42 publications

(24 citation statements)

References 2 publications

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“…6,7 The confocal devices based on tissue reflectance do not require any contrast agents, but available prototypes have had numerous technical problems and relatively low resolution, which significantly compromise in vivo imaging and clinical utility. [6][7][8][9] In contrast, confocal endomicroscopy based on tissue fluorescence uses local and/or intravenous contrast agents and generates high-quality images comparable with traditional histological examination. 5,10 Most clinical studies reported to date used a confocal fluorescence microscope integrated into the distal tip of a conventional upper endoscope (EG-3870CIK; Pentax, Tokyo, Japan) or colonoscope (EC-3870CILK; Pentax).…”

Section: Emerging Technologymentioning

confidence: 93%

Confocal laser endomicroscopy

Kantsevoy¹,

Adler²,

Conway³

et al. 2009

Gastrointestinal Endoscopy

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Section: Emerging Technologymentioning

confidence: 93%

Confocal laser endomicroscopy

Kantsevoy¹,

Adler²,

Conway³

et al. 2009

Gastrointestinal Endoscopy

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“…The two major features which identified normal and dysplastic or neoplastic tissue were visualisation of the cell nuclei characteristics, and architectural disruption of the mucosal epithelium and vasculature. [55][56][57] The absence of these features appeared to be confirmatory of normal colonic tissue. 55 A new classification for pCLE in the colon has been proposed and showed 'moderate' interobserver agreement for differentiating neoplasia from non-neoplastic tissue.…”

Section: Lower Gastrointestinal Applications Colorectal Cancermentioning

confidence: 69%

Status of confocal laser endomicroscopy in gastrointestinal disease

Humphris

Swartz

Egan

et al. 2012

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Confocal laser endomicroscopy (CLE) is an advanced imaging technique which combines conventional white light endoscopy (WLE) with an integrated or probe based confocal microscope. This allows microscopic examination of the surface epithelium and in vivo diagnosis during endoscopy. Established CLE applications include the diagnosis of Barrett's oesophagus, gastric intestinal metaplasia, coeliac disease and microscopic colitis. CLE can differentiate hyperplastic from adenomatous polyps in the colon and may obviate the need to biopsy all polyps at endoscopy. CLE is particularly helpful in surveillance endoscopy in inflammatory bowel disease where it has been shown to reduce the number of biopsies required and improve the detection of dysplasia. The future of CLE may be with new contrast agents to allow for molecular tagging and improved endoscopic diagnoses. The aim of this review is to describe the technology and techniques involved in CLE, and discuss the evolving applications in obtaining "virtual biopsy" throughout the GI tract.

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“…These include well-established features seen on light microscopy of standard histologic sections and previously published criteria for scanning confocal microscopy15, 18. The criteria used for light microscopy and confocal microscopy were utilized to identify the tissue type and evaluate the architectural and cytologic alterations.…”

Section: Methodsmentioning

confidence: 99%