Development of Variation Marker of Myzus persicae by Altitude

Abstract

This study focused on the green peach aphid, Myzus persicae, as an indicator pest in Chinese cabbage cultivation to develop a genetic marker. We hypothesized that M. persicae gene flow is related to climate change. Genetic variation was analyzed using five local populations collected at different altitudes (157 m, 296 m, 560 m, 756 m and 932 m above sea level) in Hoengseong, Pyeongchang, and Gangneung areas, plus a laboratory strain used as an outgroup. There were no differences in ecological characteristics among strains. Esterase isozyme pattern and inter-simple sequence repeat (ISSR) PCR results showed significantly different bands between laboratory and wild, local populations. However, there was no difference among local populations. Partial fragments of ribosomal RNA (rRNA) and mitochondrial cytochrome oxidase I (mtCO I) were amplified and their nucleotide sequence was analyzed. Single nucleotide polymorphisms (SNPs) were detected in internal transcribed spacer-2 (ITS-2) and mtCO I regions among the five local populations. These SNPs can be use to discriminate different populations of M. persicae to monitor gene flow.

Selection of Insecticide Resistance Markers in Field-collected Populations of Myzus persicae

“…클로프리드 저항성 수준이 많이 발달된 것을 확인하였다 (Choi et al, 2001 (Field et al, 1999;Field et al, 1996) (Bass et al, 2011a;Karunker et al, 2008;Puinean et al, 2010) Clark et al, 2001;Kim et al, 2007;Kim et al, 2012;Kim and Kwon., 2011;Kwon et al, 2004…”