Development of Validated Methods and Quantification of Curcuminoids and Curcumin Metabolites and Their Pharmacokinetic Study of Oral Administration of Complete Natural Turmeric Formulation (Cureit™) in Human Plasma via UPLC/ESI-Q-TOF-MS Spectrometry

Jude, Shintu; Amalraj, Augustine; Kunnumakkara, Ajaikumar B.; Divya, Chandradhara; Löffler, Bernd–Michael; Gopi, Sreeraj

doi:10.3390/molecules23102415

Cited by 46 publications

(26 citation statements)

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“…It is essential to understand how different sample preparation and analysis techniques impact the quantification, bioavailability, pharmacokinetic profiles, and clinical efficacy of CCMs [25,26,27,28,29,30,31,32,33], which in turn will assist in increasing understanding of curcuminoids as a potential therapeutic agent [7,26,31,34]. This review presents current research updates in the areas of CCMs formulations, and their clinical outcomes [6,14,19,20,21], sample preparation [25,26,27,28,29,31,32,33,35,36,37,38,39], and various methodologies used to analyze CCMs [25,26,33,38,39,40,41,42,43,44,45,46,47,48,49]. Sample preparation techniques developed for extracting CCMs from plants, dietary supplements, and other biological matrices, have been described.…”

Section: Introductionmentioning

confidence: 99%

Curcumin: Biological, Pharmaceutical, Nutraceutical, and Analytical Aspects

2019

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Turmeric is a curry spice that originated from India, which has attracted great interest in recent decades because it contains bioactive curcuminoids (curcumin, demethoxycurcumin, and bisdemethoxycurcumin). Curcumin (1,7-bis-(4-hydroxy-3-methoxyphenyl)-hepta-1,6-diene-3,5-dione), a lipophilic polyphenol may work as an anticancer, antibiotic, anti-inflammatory, and anti-aging agent as suggested by several in vitro, in vivo studies and clinical trials. However, poor aqueous solubility, bioavailability, and pharmacokinetic profiles limit curcumin’s therapeutic usage. To address these issues, several curcumin formulations have been developed. However, suboptimal sample preparation and analysis methodologies often hamper the accurate evaluation of bioactivities and their clinical efficacy. This review summarizes recent research on biological, pharmaceutical, and analytical aspects of the curcumin. Various formulation techniques and corresponding clinical trials and in vivo outcomes are discussed. A detailed comparison of different sample preparation (ultrasonic, pressurized liquid extraction, microwave, reflux) and analytical (FT-IR, FT-NIR, FT-Raman, UV, NMR, HPTLC, HPLC, and LC-MS/MS) methodologies used for the extraction and quantification of curcuminoids in different matrices, is presented. Application of optimal sample preparation, chromatographic separation, and detection methodologies will significantly improve the assessment of different formulations and biological activities of curcuminoids.

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Section: Introductionmentioning

confidence: 99%

Curcumin: Biological, Pharmaceutical, Nutraceutical, and Analytical Aspects

2019

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“…This is mainly due to the advantages associated with the high-resolution and accurate mass measurements attainable with these instruments. Time-of-flight (TOF) and Orbitrap mass analyzers, but especially their hybrid configurations, such as quadrupole-time-of-flight (Q-TOF), quadrupole-Orbitrap (Q-Orbitrap) and linear trap quadrupole-Orbitrap (LTQ-Orbitrap), because of their fragmentation capabilities, are the most frequently HRMS instruments used for the identification of polyphenols and curcuminoids in turmeric products [ 43 , 44 , 45 , 46 , 47 , 48 ]. In addition, ambient mass spectrometry techniques, such as direct analysis in real time-mass spectrometry (DART-MS), have also been described for the spatial localization of curcumin and the rapid screening of the chemical composition of turmeric rhizomes [ 49 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization, Classification and Authentication of Turmeric and Curry Samples by Targeted LC-HRMS Polyphenolic and Curcuminoid Profiling and Chemometrics

et al. 2020

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The importance of monitoring bioactive substances as food features to address sample classification and authentication is increasing. In this work, targeted liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) polyphenolic and curcuminoid profiles were evaluated as chemical descriptors to deal with the characterization and classification of turmeric and curry samples. The profiles corresponding to bioactive substances were obtained by TraceFinderTM software using accurate mass databases with 53 and 24 polyphenolic and curcuminoid related compounds, respectively. For that purpose, 21 turmeric and 9 curry samples commercially available were analyzed in triplicate by a simple liquid–solid extraction procedure using dimethyl sulfoxide as extracting solvent. The obtained results demonstrate that the proposed profiles were excellent chemical descriptors for sample characterization and classification by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), achieving 100% classification rates. Curcuminoids and some specific phenolic acids such as trans-cinnamic, ferulic and sinapic acids, helped on the discrimination of turmeric samples; polyphenols, in general, were responsible for the curry sample distinction. Besides, the combination of both polyphenolic and curcuminoid profiles was necessary for the simultaneous characterization and classification of turmeric and curry samples. Discrimination among turmeric species such as Curcuma longa vs. Curcuma zedoaria, as well as among different Curcuma longa varieties (Alleppey, Madras and Erode) was also accomplished.

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“…A total of 38 prototypes and 45 metabolites were identified in vivo when rats were orally administered P. cuspidatum ; however, the prototypes were all in trace levels (Fu et al, ). Gopi's group investigated the pharmacokinetics of curcuminoids and curcumin metabolites in human plasma after the oral administration of turmeric, in which curcumin and tetrahydrocurcumin displayed high exposure levels (Jude et al, ). Eight compounds were quantified in rat plasma after oral administration of H. japonicum extracts, including four organic acids and four flavones (Pang, Tian, Mi, Wang, & Xu, ).…”

Section: Introductionmentioning

confidence: 99%

Systematic investigation on the chemical basis of anti‐NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

Liu

Sun

Tian

et al. 2020

Biomedical Chromatography

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Qushi Huayu Fang (QHF) is a clinic-empirical prescription for treating non-alcoholic fatty liver disease (NAFLD) in China, which is composed of five herbs. However, the bioactive constituents responsible for the efficacy of QHF remain unclear. Thus, a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method was established and adopted to identify the constituents of QHF, and profile its metabolism in vivo and in vitro. Among the 66 constituents in QHF, only 14 compounds of six structural types were absorbed, and 34 metabolites were generated through eight metabolic pathways. A total of 20 metabolites were first reported, including four organic acids, one iridoid, two flavones, five naphthols, three anthraquinones, and five stilbenes. Glucuronidation and sulfation were the main metabolic pathways, and the intestinal metabolism played an important role in the biotransformation of QHF. Many compounds, especially those detected in the liver, the target organ of QHF, were reported to display the anti-NAFLD activity. This is the first study to explore the constituents of QHF and its metabolism in vivo and in vitro, thus realizing the first step to clarify the chemical basis of QHF qualitatively, and laying the foundation for further research on the anti-NAFLD mechanism of QHF. K E Y W O R D S HPLC-Q-TOF, intestinal bacteria, liver, metabolism, Qushi Huayu FangAbbreviations: HPLC-Q-TOF, high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry; IRE1α, inositol-requiring transmembrane kinase/endoribonuclease 1α; NAFLD, non-alcoholic fatty liver disease; Nrf-2, nuclear erythroid 2-related factor 2; PI3K, phosphatidylinositol 3-kinase; PPARα, peroxisomal proliferator-activated receptor α; QHF, Qushi Huayu Fang; SD, Sprague Dawley; SREBP-1c, sterol regulatory element-binding protein-1c; TCM, traditional Chinese medicines; TNF-α, tumor necrosis factor-α; XBP1s, X-box binding protein 1; YCHD, Yinchenhao Decoction. # Huan Liu and Zhaolin Sun contributed equally to this study.

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Development of Validated Methods and Quantification of Curcuminoids and Curcumin Metabolites and Their Pharmacokinetic Study of Oral Administration of Complete Natural Turmeric Formulation (Cureit™) in Human Plasma via UPLC/ESI-Q-TOF-MS Spectrometry

Cited by 46 publications

References 21 publications

Curcumin: Biological, Pharmaceutical, Nutraceutical, and Analytical Aspects

Curcumin: Biological, Pharmaceutical, Nutraceutical, and Analytical Aspects

Characterization, Classification and Authentication of Turmeric and Curry Samples by Targeted LC-HRMS Polyphenolic and Curcuminoid Profiling and Chemometrics

Systematic investigation on the chemical basis of anti‐NAFLD Qushi Huayu Fang. Part 1: A study of metabolic profiles in vivo and in vitro by high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry

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