Development of UPLC-MS/MS method for analyzing phorate: application to wastewater treatment

Pretreatment and analysis techniques development of TKIs in biological samples for pharmacokinetic studies and therapeutic drug monitoring

“…The parameter was optimized using a 3-level factorial design approach, and the best results were obtained under the following conditions: C 18 column, ammonium format buffer consisting of acetonitrile (28.19:71.81, V / V ), and buffer with pH 3. This optimized method was successfully applied to a pharmacokinetic study, demonstrating high recovery (99.91%), specificity (99.94%), precision (RSD < 0.543%), and robustness (RSD < 0.500%) ( Table S1 ) [ 2 , 3 , 5 , 8 , 12 , [15] , [16] , [17] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [37] , [38] , [39] , 41 , [48] , [49] , [50] , 52 , 54 , 55 , 61 , 63 , 64 , 76 , 80 , 83 , 84 , 86 , [88] , [89] , [90] , [91] , [92] , [93] , [94] , [95] , [97] , [98] , [99] , [100] , [101] , [102] , [103] , [104] , [105] , 109 , [111] , [112] , [113] , [114] ,…”

“…Commonly used protein precipitants include methanol, acetonitrile, dichloromethane, among others. Acetonitrile exhibits superior performance compared to methanol and other organic solvents like dichloromethane as it promotes enhanced protein precipitation and maximizes the recovery of strongly protein-bound imatinib [ [30] , [31] , [32] ].…”