Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin B1 in food products

The Optimization of a Lateral Flow Immunoassay for Detection of Aflatoxin B1 in Potable Water Samples

“…As aflatoxin B1 is considered to be one of the most dangerous mycotoxins, it is listed as a group I carcinogen by the International Agency for Research in Cancer [6]. The EU has set maximum permitted levels for aflatoxin B1 in nuts, dried fruits, cereals and spices to range from 2-12 μg/kg, while the maximum permitted level for aflatoxin B1 in infant foods is set at 0.1 μg/kg [7]. Methods developed have mainly focused on detection in the context of food [8,9].…”

Immunochromatographic Assay for Ultrasensitive Detection of Aflatoxin B1 in Maize by Highly Luminescent Quantum Dot Beads

“…For example, Yu et al reported a direct competitive chemiluminescent ELISA for determination of AFB 1 with a limit of detection (LOD) of 1.5 pg mL –1 . 6 Hu et al developed a nonfouling polymer brush-based fluorescent competitive immunoassay microarray to detect multiplex mycotoxins, including AFB 1 with an LOD of 4 pg mL –1 . 7 Deng et al reported an ultrasensitive immunoassay using silica photonic crystal microsphere suspension array method for multiplex mycotoxin detection with an LOD at 0.5 pg mL –1 for AFB 1 .…”

Rapid detection method for aflatoxin B1 in soybean sauce based on fluorescent microspheres probe

“…Several analytical methods, such as HPLC (Ding, Li, Bai, & Zhou, 2012), LCeMS/MS (Soleimany, Jinap, & Abas, 2012) and ELISA (Yu, Gribas, Vdovenko, & Sakharov, 2013), are currently available for AFB 1 determination. However, these methods involve tedious pretreatment, which is not suitable for non-laboratory applications.…”