Development of ultra-thin chips for immobilization of Caenorhabditis elegans in microfluidic channels during irradiation and selection of buffer solution to prevent dehydration

Suzuki, Michiyo; Sakashita, Tetsuya; Hattori, Yuya; Yokota, Yuichiro; Kobayashi, Yasuhiko; Funayama, Tomoo

doi:10.1016/j.jneumeth.2018.05.025

Cited by 13 publications

(30 citation statements)

References 12 publications

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“…We investigated the effects of targeted microbeam irradiation using carbon ion ( 12 C 6+ ) particles accelerated by an azimuthally varying field cyclotron installed at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) facility of QST-Takasaki. Irradiation generally followed previously described methods [16,18]. We delivered targeted microbeam irradiation using a collimating microbeam system [18].…”

Section: Evaluation Of Carbon-ion Collimationmentioning

confidence: 99%

“…Before biological experiments using C. elegans, we evaluated the distribution of the collimated carbon-ion beam using a micro-aperture with a 60-µm and 20-µm diameter. We irradiated an ion-track detector CR-39 film (Solid State Nuclear Track Detector HARZLAS TNF-1, Fukuvi Chemical Industry, Fukui, Japan) with 10 or 100 ion particles and observed the resulting etch pits as described previously [10,16].…”

Section: Evaluation Of Carbon-ion Collimationmentioning

confidence: 99%

“…In the present study, we aimed to examine the irradiated area-and dose-dependent effects of CNS-targeted irradiation on the motility of C. elegans using a collimating microbeam system at QST-Takasaki (Gunma, Japan) [15]. We previously developed an ion-penetrable, ultra-thin polydimethylsiloxane (PDMS) microfluidic chip (Figure 1), named the Worm Sheet, for on-chip immobilization for targeted microbeam irradiation of individual live C. elegans [16]. Here, we propose a novel analysis that combines targeted irradiation with an on-chip behavioral imaging approach using a Worm Sheet that allows the rapid, direct, multiplex measurement of individual animals before and after irradiation.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, this system can replace various micro-aperture cylinders with holes of a specific size (e.g., 20-, 120-, or 250-µm diameter) to form ion beams of a specific size. In addition to a micro-aperture (beam exit) with a 20-µm diameter used in previous studies [10,16], we used a new micro-aperture of 60-µm diameter (equal to the body width of C. elegans), which can apply irradiation consisting of carbon ions, whose range is less than 1 mm in water. Using the two micro-apertures, we investigated the area-dependent effects of targeted irradiation.…”

Section: Introductionmentioning

confidence: 99%

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Targeted Central Nervous System Irradiation of Caenorhabditis elegans Induces a Limited Effect on Motility

Suzuki

Soh

Yamashita

et al. 2020

Biology

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To clarify the tissue responsible for a biological function, that function can be experimentally perturbed by an external stimulus, such as radiation. Radiation can be precisely and finely administered and any subsequent change in function examined. To investigate the involvement of the central nervous system (CNS) in Caenorhabditis elegans’ locomotion, we irradiated a limited 20-µm-diameter area of the CNS with a single dose and evaluated the resulting effects on motility. However, whether irradiated area (beam size)-dependent or dose-dependent effects on motility occur via targeted irradiation remain unknown. In the present study, we examined the irradiated area- and dose-dependent effects of CNS-targeted irradiation on the motility of C. elegans using a collimating microbeam system and confirmed the involvement of the CNS and body-wall muscle cells around the CNS in motility. After CNS-targeted microbeam irradiation, C. elegans’ motility was assayed. The results demonstrated a dose-dependent effect of CNS-targeted irradiation on motility reflecting direct effects on the irradiated CNS. In addition, when irradiated with 1000-Gy irradiation, irradiated area (beam size)-dependent effects were observed. This method has two technical advantages: Performing a series of on-chip imaging analyses before and after irradiation and targeted irradiation using a distinct ion-beam size.

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Section: Evaluation Of Carbon-ion Collimationmentioning

confidence: 99%

Section: Evaluation Of Carbon-ion Collimationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Targeted Central Nervous System Irradiation of Caenorhabditis elegans Induces a Limited Effect on Motility

Suzuki

Soh

Yamashita

et al. 2020

Biology

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“…We identified a suitable buffer solution to maintain good worm conditions in the channel. Using this method, irradiating a specific region of C. elegans with the exact number of heavy ions is now possible as well as analyzing the immediate response on locomotive activity [23][24][25].…”

Section: Manual Targeting Irradiationmentioning

confidence: 99%

Heavy-Ion Microbeams for Biological Science: Development of System and Utilization for Biological Experiments in QST-Takasaki

Funayama

2019

QuBS

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Target irradiation of biological material with a heavy-ion microbeam is a useful means to analyze the mechanisms underlying the effects of heavy-ion irradiation on cells and individuals. At QST-Takasaki, there are two heavy-ion microbeam systems, one using beam collimation and the other beam focusing. They are installed on the vertical beam lines of the azimuthally-varying-field cyclotron of the TIARA facility for analyzing heavy-ion radiation effects on biological samples. The collimating heavy-ion microbeam system is used in a wide range of biological research not only in regard to cultured cells but also small individuals, such as silkworms, nematode C. elegans, and medaka fish. The focusing microbeam system was designed and developed to perform more precise target irradiation that cannot be achieved through collimation. This review describes recent updates of the collimating heavy ion microbeam system and the research performed using it. In addition, a brief outline of the focusing microbeam system and current development status is described.

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An irradiation device for biological targets using focused microbeams of cyclotron-accelerated heavy ion

Funayama

Sakashita

Suzuki

et al. 2020

Nuclear Instruments and Methods in Physics Research Section B:

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Development of ultra-thin chips for immobilization of Caenorhabditis elegans in microfluidic channels during irradiation and selection of buffer solution to prevent dehydration

Cited by 13 publications

References 12 publications

Targeted Central Nervous System Irradiation of Caenorhabditis elegans Induces a Limited Effect on Motility

Targeted Central Nervous System Irradiation of Caenorhabditis elegans Induces a Limited Effect on Motility

Heavy-Ion Microbeams for Biological Science: Development of System and Utilization for Biological Experiments in QST-Takasaki

An irradiation device for biological targets using focused microbeams of cyclotron-accelerated heavy ion

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