Development of two-dimensional qualitative visualization method for isoflavones secreted from soybean roots using sheets with immobilized bovine serum albumin

Onodera, Takeshi; Miyazaki, Haruna; Li, Xinzhu; Wang, Jin; Nakayasu, Masaru; Yatabe, Rui; Tahara, Yusuke; Hosoki, Ai; Sakurai, Nozomu; Sugiyama, Akifumi

doi:10.1016/j.bios.2021.113705

Cited by 3 publications

(1 citation statement)

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“…We previously used the fluorescence of bovine serum albumin (BSA) to visualize isoflavones such as daidzein secreted from soybean roots and a surface plasmon resonance sensor using hetero-core fibers to detect plant-related compounds. (2)(3)(4) Other than pH meters, immunochromatography is the most used measurement method to detect chemical compounds in the field. (5)(6)(7) For plant-related substances, test strips for cadmium are commercially available, and other chemicals can also be rapidly evaluated.…”

Section: Introductionmentioning

confidence: 99%

Development of Monoclonal Antibodies and Immunochromatographic Test Strips Applying Indirect Competitive Method for Daidzein Detection

Onodera,

Ajisaka,

Ogawa

et al. 2024

Sensors and Materials

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In this study, we aimed to detect daidzein secreted in the soybean rhizosphere using an indirect competitive immunochromatographic assay. A specific anti-daidzein monoclonal antibody was obtained through the establishment of a hybridoma. The antibody exhibited a detection limit of 5 ppb daidzein and showed strong cross-reactivity with daidzein, as determined by an indirect competitive method using a surface plasmon resonance sensor. Through the findings, a unique binding pattern differing from those of genistein and other saponins was observed. Enhanced sensitivity with the ability to detect daidzein concentrations as low as 10 nM (2.5 ng/mL) was achieved using immunochromatographic test strips with modified gold nanoparticles.

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