Development of Transgenic Sugarcane Resistant to Sugarcane Borer

Cristofoletti, Plı́nio T.; Kemper, Edson L.; Capella, A.; Carmago, Sandra R.; Cazoto, Juliana L.; Ferrari, Fernanda; Galvan, Tederson L.; Gauer, Luciane; Monge, Gustavo A.; Nishikawa, Marcelo Akira Naime; Santos, Nícolas Zendonadi dos; Semeão, Altair Arlindo; Silva, Leandro; Willse, Alan; Zanca, Almir S.; Edgerton, Michael D.

doi:10.1007/s12042-018-9198-y

Cited by 35 publications

(25 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Arvinth et al (2010) telah menggunakan pyramiding kombinasi gen cry1Ab dan lektin snowdrop (Galanthus nivalis agglutinin/GNA) untuk mengembangkan tebu tahan hama penggerek di India. Sementara, Cristofoletti et al (2018) telah mengembangkan tebu tahan hama penggerek di Brazil dengan menggunakan gen fusi cry1Ab dan cry2A.…”

Section: Pendahuluanunclassified

Development of Transgenic Sugarcane Resistant to Stem Borer by Transforming cry1Ab-cry1Ac Fusion Gene through Agrobacterium tumefaciens Transformation Method

2021

View full text Add to dashboard Cite

<p>Bulu Lawang (BL) is a sugarcane variety preferred by farmers in Indonesia due to its high yield, but this cultivar is susceptible to shoot and stem borer insect pests. Genetic engineering using cry1Ab and cry1Ac fusion gene is an effort to generate BL varieties resistant to the insect pests. This study aimed to 1) transform T-DNA containing cry1Ab-cry1Ac fusion gene into sugarcane genome by using Agrobacterium tumefaciens method, 2) obtain selection media composition of callus transformants, and 3) obtain transformation efficiency comparison of A. tumefaciens strains EHA105 and GV3101. The research was conducted at the Laboratory of Cell and Tissue Biology, the Laboratory of Molecular Biology, and the greenhouse of the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor from March to August 2019. Research activities consisted of four parts namely 1) callus induction and Agrobacterium culture preparation containing pCambia5300-cry1Ab-cry1Ac ///// pCambia-5300_OaRbcS-prom-cTP-cry1Ab-cry1Ac plasmid, 2) callus incubation in cocultivation and resting media, 3) selection and differentiation of shoots on regeneration media, and 4) molecular analysis using PCR method. Results showed that the composition of media, both for selection and regeneration processes of putative plant transformants, was the key to the success of this experiment. A. tumefaciens strain EHA105 resulted in higher transformation efficiency (11.1%) compared to that of strain GV3101 (9.0%). Molecular analysis showed that cryIAb-cryIAc fusion gene was successfully inserted into the sugarcane genome suggesting that the transgenic plant containing cry1Ab-cry1Ac fusion gene was obtained. The putative transgenic plants need further assay through bioassay tests to verify its resistance phenotype to the insect pests.</p>

show abstract

Section: Pendahuluanunclassified

Development of Transgenic Sugarcane Resistant to Stem Borer by Transforming cry1Ab-cry1Ac Fusion Gene through Agrobacterium tumefaciens Transformation Method

2021

View full text Add to dashboard Cite

show abstract

“…Currently, transgenic sugarcane varieties resistant to D. saccharalis containing genes from Bacillus AC Tomaz et al thuringiensis bacteria (Bt) have been released in Brazil (MAPA 2019). However, there is the possibility of the rapid evolution of sugarcane borer resistance to Bt proteins under large-scale field use (Girón-Pérez et al 2014, Cristofoletti et al 2018. The development of sugarcane genotypes resistant to the borer through conventional breeding could enable more durable resistance and could be performed in sugarcane breeding programs with no access to transgenic technologies.…”

Section: Introductionmentioning

confidence: 99%

Genetic parameters and selection of sugarcane for borer resistance in the advanced selection stage

Tomaz

Gonçalves

Resende

et al. 2020

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

This study aimed to investigate genetic parameters and selection strategies for sugarcane resistance to borer and determine the effects of selection for borer resistance on yield traits. The infestation index by borer (IIB) and yield traits were assessed over 3 years in 35 sugarcane clones planted in four locations in southeastern Brazil. There was no genotype × environment (year or location) interaction for borer resistance. Consequently, selection may be performed at only one location and year or by the mean of clones in several locations and/or years. The borer population affected the genotypic variance for borer resistance but when genotypic variance was significant, heritability was elevated and suitable genetic gain was achieved. Selection for borer resistance reduced the genetic gains for tons of cane per hectare, theoretical recoverable sugar, and tons of sugar per hectare; however, satisfactory selection gains for both borer resistance and yield were achieved using selection index.

show abstract

“…Due to the intrinsic difficulties of traditional sugarcane breeding, the development of cultivars by genetic modification, including gene editing, offers a great potential as it can overcome some of the limitations (Brinegar et al, 2017;Hilscher et al, 2017;Ricroch et al, 2017;Wang et al, 2017;Cristofoletti et al, 2018;Eriksson et al, 2018;Nerkar et al, 2018;Zhang et al, 2018;Khan et al, 2019). The commercial release of GM cultivars is conditioned to the assessment of biosafety risks (Cheavegatti- Gianotto et al, 2011), to meet the requirements of the national regulatory systems (Jaffe, 2004;Eriksson et al, 2018;Khan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil

Bressan

Carvalho

Borges

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

Development of Transgenic Sugarcane Resistant to Sugarcane Borer

Cited by 35 publications

References 33 publications

Development of Transgenic Sugarcane Resistant to Stem Borer by Transforming cry1Ab-cry1Ac Fusion Gene through Agrobacterium tumefaciens Transformation Method

Development of Transgenic Sugarcane Resistant to Stem Borer by Transforming cry1Ab-cry1Ac Fusion Gene through Agrobacterium tumefaciens Transformation Method

Genetic parameters and selection of sugarcane for borer resistance in the advanced selection stage

Assessment of Gene Flow to Wild Relatives and Nutritional Composition of Sugarcane in Brazil

Contact Info

Product

Resources

About