Development of transformation vectors for the production of potentially high oleate transgenic oil palm

Masani, Mat Yunus Abdul; Parveez, Ghulam Kadir Ahmad

doi:10.2225/vol11-issue3-fulltext-7

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…Other targets are increasing specialty fatty acids such as ricinoleic, palmitoleic, and stearic, increasing lycopene content as well as synthesizing biodegradable plastics. In order to achieve the above target, construction of transformation vectors for various products have (Yunus and Kadir, 2008; Yunus et al, 2008; Masani et al, 2009) and are being carried out before they could be used to transform oil palm. Being a perennial tree and having a long regeneration time of around 7–10 years (Rajanaidu and Jalani, 1995) the use of conventional breeding, with the need of crossing and back-crossing, will require nearly half a decade to introduce a new trait into this species.…”

Section: Introductionmentioning

confidence: 99%

Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

et al. 2015

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DOGR1, which encodes 2-deoxyglucose-6-phosphate phosphatase, has been used as a selectable marker gene to produce transgenic plants. In this study, a transformation vector, pBIDOG, which contains the DOGR1 gene, was transformed into oil palm embryogenic calli (EC) mediated by Agrobacterium tumefaciens strain LBA4404. Transformed EC were exposed to 400 mg l-1 2-deoxyglucose (2-DOG) as the selection agent. 2-DOG resistant tissues were regenerated into whole plantlets on various regeneration media containing the same concentration of 2-DOG. The plantlets were later transferred into soil and grown in a biosafety screenhouse. PCR and subsequently Southern blot analyses were carried out to confirm the integration of the transgene in the plantlets. A transformation efficiency of about 1.0% was obtained using DOGR1 gene into the genome of oil palm. This result demonstrates the potential of using combination of DOGR1 gene and 2-DOG for regenerating transgenic oil palm.

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Section: Introductionmentioning

confidence: 99%

Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

et al. 2015

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Oil Palm (Elaeis spp.) Breeding in Malaysia

Malike

Amiruddin

Yaakub

et al. 2019

Advances in Plant Breeding Strategies: Industrial and Food Crops

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Biotechnology of oil palm: strategies towards manipulation of lipid content and composition

et al. 2014

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Oil palm is a major economic crop for Malaysia. The major challenges faced by the industry are labor shortage, availability of arable land and unstable commodity price. This has caused the industry to diversify its applications into higher value products besides increasing its yield. While conventional breeding has its limitations, biotechnology was identified as one of the tools for overcoming the above challenges. Research on biotechnology of oil palm began more than two decades ago leveraging a multidisciplinary approach involving biochemical studies, gene and promoter isolation, transformation vector construction and finally genetic transformation to produce the targeted products. The main target of oil palm biotechnology research is to increase oleic acid in the mesocarp. Other targets are stearic acid, palmitoleic acid, ricinoleic acid, lycopene (carotenoid) and biodegradable plastics. Significant achievements were reported for the biochemical studies, isolation of useful oil palm genes and characterization of important promoters. A large number of transformation constructs for various targeted products were successfully produced using the isolated oil palm genes and promoters. Finally transformation of these constructs into oil palm embryogenic calli was carried out while the regeneration of transgenic oil palm harboring the useful genes is in progress.

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Development of transformation vectors for the production of potentially high oleate transgenic oil palm

Cited by 3 publications

References 17 publications

Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

Evaluation on the effectiveness of 2-deoxyglucose-6-phosphate phosphatase (DOGR1) gene as a selectable marker for oil palm (Elaeis guineensis Jacq.) embryogenic calli transformation mediated by Agrobacterium tumefaciens

Oil Palm (Elaeis spp.) Breeding in Malaysia

Biotechnology of oil palm: strategies towards manipulation of lipid content and composition

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