Development of three X-linked tetrameric microsatellite markers for forensic purposes

Abstract

In this study, we obtained sequence and population genetic data for three X-linked short tandem repeat markers (X-STRs; DXS7129, DXS2500, G10583). We investigated their population genetics and estimated their forensic parameters in 214 healthy unrelated individuals from the Han population of Northern China (105 males and 109 females). We showed that DXS2500 and G10583 were highly polymorphic and thus have potential for application in forensic medicine. We also estimated the overall linkage disequilibrium between pairs of loci, specific multiallelic or interallelic associations, and haplotype frequencies in males. We showed that the three X-STR loci segregate as stable haplotype blocks; this could be a powerful tool for haplotype analysis in kinship testing.

Allele and haplotype diversity of new multiplex of 19 ChrX‐STR loci in Han population from Guanzhong region (China)

“…However, autosomal STRs may not be effective in some deficiency paternity cases, such as the father–daughter testing that the DNA of mother is not available, and the full sisters or paternal sisters testing that the DNA of their biological father is not available. X‐chromosomal STRs were proved to be effective in these cases , because one of two X‐chromosomes of daughter is certainly inherited from her biological father and the sisters share the same paternal X‐chromosome. However, only several studies were reported with some available multiplex amplification systems, including Mentype® Argus X‐8 kit (Biotype AG, Dresden, Germany), Investigator Argus X‐12 (Qiagen, Hilden, Germany) and ZJGA‐X12 STR kit (AGCU ScienTech, Wuxi, Jiangsu, China) in the past .…”