Development of thick paste-like inks based on superconcentrated gelatin/alginate for 3D printing of scaffolds with shape fidelity and stability

Curti, Filis; Drăgușin, Diana-Maria; Serafim, Andrada; Iovu, Horia; Stancu, Izabela‐Cristina

doi:10.1016/j.msec.2021.111866

Cited by 27 publications

(13 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shape fidelity and integrity are two major challenges in the 3D printing process with hydrogel-based biomaterial inks, as they can affect the overall performance of 3D structures. [38][39][40][41] The material's printability degree, defined as its ability to deposit and solidify, is a key consideration in fabricating a desired scaffold. 42 vol/vol of the 35% Aloe vera solution) proved to be suitable for 3D…”

Section: Print Quality Assessment and Rheological Evaluationmentioning

confidence: 99%

Aloe vera‐based biomaterial ink for 3D bioprinting of wound dressing constructs

Ceballos‐Santa,

Sierra,

Zalbidea

et al. 2024

J Biomed Mater Res

View full text Add to dashboard Cite

This study emphasizes the development of a multifunctional biomaterial ink for wound healing constructs. The biomaterial ink benefits from Aloe vera's intrinsic biocompatible, biodegradable, antioxidant, antimicrobial, anti‐inflammatory, and immunomodulatory attributes, thus alleviating the need for supplementary substances employed to combat infections and stimulate tissue regeneration. Moreover, this biomaterial ink seeks to address the scarcity of standardized printable materials possessing adequate biocompatibility and physicochemical properties, which hinder its widespread clinical adoption. The biomaterial ink was synthesized via ionic crosslinking to enhance its rheological and mechanical characteristics. The findings revealed that Aloe vera substantially boosted the hydrogel's viscoelastic behavior, enabling superior compressive modulus and the extrusion of fine filaments. The bioprinted constructs exhibited desirable resolution and mechanical strength while displaying a porous microstructure analogous to the native extracellular matrix. Biological response demonstrated no detrimental impact on stem cell viability upon exposure to the biomaterial ink, as confirmed by live/dead assays. These outcomes validate the potential of the developed biomaterial ink as a resource for the bioprinting of wound dressings that effectively foster cellular proliferation, thereby promoting enhanced wound healing by leveraging Aloe vera's inherent properties.

show abstract

Section: Print Quality Assessment and Rheological Evaluationmentioning

confidence: 99%

Aloe vera‐based biomaterial ink for 3D bioprinting of wound dressing constructs

Ceballos‐Santa,

Sierra,

Zalbidea

et al. 2024

J Biomed Mater Res

View full text Add to dashboard Cite

show abstract

“…CT-based evaluation is also widely run for scaffolds fabricated through the 3D printing of a variety of materials, such as natural or synthetic polymers [73][74][75] and polymerbased composites [76][77][78], ceramic powders [79][80][81][82], and glasses [83]. A method article thoroughly describes a micro-CT scanning protocol suitable for Ti6Al4V metal powders with a mean size of 1.4 µm.…”

Section: Visualization Of Architectural Features Through Ct Imagingmentioning

confidence: 99%

Computed Tomography as a Characterization Tool for Engineered Scaffolds with Biomedical Applications

et al. 2021

Self Cite

View full text Add to dashboard Cite

The ever-growing field of materials with applications in the biomedical field holds great promise regarding the design and fabrication of devices with specific characteristics, especially scaffolds with personalized geometry and architecture. The continuous technological development pushes the limits of innovation in obtaining adequate scaffolds and establishing their characteristics and performance. To this end, computed tomography (CT) proved to be a reliable, nondestructive, high-performance machine, enabling visualization and structure analysis at submicronic resolutions. CT allows both qualitative and quantitative data of the 3D model, offering an overall image of its specific architectural features and reliable numerical data for rigorous analyses. The precise engineering of scaffolds consists in the fabrication of objects with well-defined morphometric parameters (e.g., shape, porosity, wall thickness) and in their performance validation through thorough control over their behavior (in situ visualization, degradation, new tissue formation, wear, etc.). This review is focused on the use of CT in biomaterial science with the aim of qualitatively and quantitatively assessing the scaffolds’ features and monitoring their behavior following in vivo or in vitro experiments. Furthermore, the paper presents the benefits and limitations regarding the employment of this technique when engineering materials with applications in the biomedical field.

show abstract

“…However, a limitation of synthetic polymers is their lack of inherent cell adhesive motifs in their chemical structure, which can inhibit cell adhesion. Consequently, when implanted in vivo , these materials may trigger a significant inflammatory response. − …”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Extrusion Printed Urinary Specific Grafts: Mechanistic Insights into Buildability and Biophysical Properties

Chowdhury,

Mondal,

Ratnayake

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

The compositional formulations and the optimization of process parameters to fabricate hydrogel scaffolds with urological tissue-mimicking biophysical properties are not yet extensively explored, including a comprehensive assessment of a spectrum of properties, such as mechanical strength, viscoelasticity, antimicrobial property, and cytocompatibility. While addressing this aspect, the present work provides mechanistic insights into process science, to produce shape-fidelity compliant alginate-based biomaterial ink blended with gelatin and synthetic nanocellulose. The composition-dependent pseudoplasticity, viscoelasticity, thixotropy, and gel stability over a longer duration in physiological context have been rationalized in terms of intermolecular hydrogen bonding interactions among the biomaterial ink constituents. By varying the hybrid hydrogel ink composition within a narrow compositional window, the resulting hydrogel closely mimics the natural urological tissue-like properties, including tensile stretchability, compressive strength, and biophysical properties. Based on the printability assessment using a critical analysis of gel strength, we have established the buildability of the acellular hydrogel ink and have been successful in fabricating shape-fidelity compliant urological patches or hollow cylindrical grafts using 3D extrusion printing. Importantly, the new hydrogel formulations with good hydrophilicity, support fibroblast cell proliferation and inhibit the growth of Gram-negative E. coli bacteria. These attributes were rationalized in terms of nanocellulose-induced physicochemical changes on the scaffold surface. Taken together, the present study uncovers the process-science-based understanding of the 3D extrudability of the newly formulated alginate-gelatin-nanocellulose-based hydrogels with urological tissue-specific biophysical, cytocompatibility, and antibacterial properties.

show abstract

Development of thick paste-like inks based on superconcentrated gelatin/alginate for 3D printing of scaffolds with shape fidelity and stability

Cited by 27 publications

References 37 publications

Aloe vera‐based biomaterial ink for 3D bioprinting of wound dressing constructs

Aloe vera‐based biomaterial ink for 3D bioprinting of wound dressing constructs

Computed Tomography as a Characterization Tool for Engineered Scaffolds with Biomedical Applications

Three-Dimensional Extrusion Printed Urinary Specific Grafts: Mechanistic Insights into Buildability and Biophysical Properties

Contact Info

Product

Resources

About