Development of thermo-sensitive cytoplasmic male sterile (TCMS) lines of wheat characterized by complete male sterility at lower-temperatures and partially restored fertility at higher-temperatures

Song, Xi Yue; Zhang, Ling Li; Zeng, Jun Li; Qian, Huan Huan; Li, Hong Bin; He, Bei Ru

doi:10.1007/s10681-013-0871-9

Cited by 13 publications

(14 citation statements)

References 9 publications

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“…The wheat CMS line KTM3315A was developed via consecutive backcrosses with TM3315B as the donor parent [ 9 ]. KTM3315A, a TCMS line, was developed by Northwest A&F University, China, where its thermo-sensitive fertility depends on the recessive nuclear gene rfv 1 ma from the 1BS chromosome of Triticum macha [ 15 ].…”

Section: Resultsmentioning

confidence: 99%

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Identification of Proteins Involved in Carbohydrate Metabolism and Energy Metabolism Pathways and Their Regulation of Cytoplasmic Male Sterility in Wheat

Geng

Yang

et al. 2018

IJMS

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Cytoplasmic male sterility (CMS) where no functional pollen is produced has important roles in wheat breeding. The anther is a unique organ for male gametogenesis and its abnormal development can cause male sterility. However, the mechanisms and regulatory networks related to plant male sterility are poorly understood. In this study, we conducted comparative analyses using isobaric tags for relative and absolute quantification (iTRAQ) of the pollen proteins in a CMS line and its wheat maintainer. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology classifications, metabolic pathways and transcriptional regulation networks using Blast2GO. We identified 5570 proteins based on 23,277 peptides, which matched with 73,688 spectra, including proteins in key pathways such as glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase and 6-phosphofructokinase 1 in the glycolysis pathway, isocitrate dehydrogenase and citrate synthase in the tricarboxylic acid cycle and nicotinamide adenine dinucleotide (NADH)-dehydrogenase and adenosine-triphosphate (ATP) synthases in the oxidative phosphorylation pathway. These proteins may comprise a network that regulates male sterility in wheat. Quantitative real time polymerase chain reaction (qRT-PCR) analysis, ATP assays and total sugar assays validated the iTRAQ results. These DAPs could be associated with abnormal pollen grain formation and male sterility. Our findings provide insights into the molecular mechanism related to male sterility in wheat.

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Section: Resultsmentioning

confidence: 99%

“…Currently, a two-line system involving thermo-sensitive CMS (TCMS) is being studied to assess its possible application in hybrid wheat breeding. This system can maintain and multiply male sterile lines via self-pollination, which is much simpler and cheaper than a three-line system in wheat [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Proteins Involved in Carbohydrate Metabolism and Energy Metabolism Pathways and Their Regulation of Cytoplasmic Male Sterility in Wheat

Geng

Yang

et al. 2018

IJMS

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“…We have observed some association between photoperiod and plant location; however, our results demonstrated that fertility was not influenced by day-length as in other CMS systems [ 20 ]. For this reason, we cannot reliably separate the effect on fertility of factors ‘Location’ and ‘Length day’ and more studies on the effect of photoperiod on fertility in wheat with H. chilense cytoplasm with and without nuclear genes restorers are needed.…”

Section: Discussionmentioning

confidence: 44%

“…Thermo-sensitive cytoplasmic male sterility lines have been frequently observed in other CMS systems. Indeed, this is the basis for the development of ‘two line hybrid systems’ in which temperature at anthesis determines fertility phenotype [ 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

Contribution of Chromosomes 1HchS and 6HchS to Fertility Restoration in the Wheat msH1 CMS System under Different Environmental Conditions

et al. 2015

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Exploiting hybrid wheat heterosis has been long pursued to increase crop yield, stability and uniformity. Cytoplasmic male sterility (CMS) systems based in the nuclear-cytoplasmic incompatible interactions are a classic way for hybrid seed production, but to date, no definitive system is available in wheat. The msH1 CMS system results from the incompatibility between the nuclear genome of wheat and the cytoplasmic genome of the wild barley Hordeum chilense. Fertility restoration of the CMS phenotype was first associated with the disomic addition of the short arm of chromosome 6H from H. chilense. In further studies it was observed that chromosome arm 1HchS was also implicated, and the combination of genes in both chromosome arms restored fertility more efficiently. In this work we aim to dissect the effect of each chromosome in fertility restoration when combined in different genomic backgrounds and under different environmental conditions. We propose a model to explain how restoration behaves in the msH1 system and generate valuable information necessary to develop an efficient system for hybrid wheat production.

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“…The sterility of KTM3315A is partially restored when plants are grown at temperatures above 20°C during the growth stages 45–52 (Song et al, 2013). Male fertility is not correlated with day length.…”

Section: Introductionmentioning

confidence: 99%

Identification of Candidate Genes and Biosynthesis Pathways Related to Fertility Conversion by Wheat KTM3315A Transcriptome Profiling

Duan

Gan

et al. 2017

Front. Plant Sci.

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The Aegilops kotschyi thermo-sensitive cytoplasmic male sterility (K-TCMS) system may facilitate hybrid wheat (Triticum aestivum L.) seed multiplication and production. The K-TCMS line is completely male sterile during the normal wheat-growing season, whereas its fertility can be restored in a high-temperature environment. To elucidate the molecular mechanisms responsible for male sterility/fertility conversion and candidate genes involved with pollen development in K-TCMS, we employed RNA-seq to sequence the transcriptomes of anthers from K-TCMS line KTM3315A during development under sterile and fertile conditions. We identified 16840 differentially expressed genes (DEGs) in different stages including15157 known genes (15135 nuclear genes and 22 plasmagenes) and 1683 novel genes. Bioinformatics analysis identified possible metabolic pathways involved with fertility based on KEGG pathway enrichment of the DEGs expressed in fertile and sterile plants. We found that most of the genes encoding key enzyme in the phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were significant upregulated in uninucleate, binuclate or trinucleate stage, which both interact with MYB transcription factors, and that link between all play essential roles in fertility conversion. The relevant DEGs were verified by quantitative RT-PCR. Thus, we suggested that phenylpropanoid biosynthesis and jasmonate biosynthesis pathways were involved in fertility conversion of K-TCMS wheat. This will provide a new perspective and an effective foundation for the research of molecular mechanisms of fertility conversion of CMS wheat. Fertility conversion mechanism in thermo-sensitive cytoplasmic male sterile/fertile wheat involves the phenylpropanoid biosynthesis pathway, jasmonate biosynthesis pathway, and MYB transcription factors.

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Development of thermo-sensitive cytoplasmic male sterile (TCMS) lines of wheat characterized by complete male sterility at lower-temperatures and partially restored fertility at higher-temperatures

Cited by 13 publications

References 9 publications

Identification of Proteins Involved in Carbohydrate Metabolism and Energy Metabolism Pathways and Their Regulation of Cytoplasmic Male Sterility in Wheat

Identification of Proteins Involved in Carbohydrate Metabolism and Energy Metabolism Pathways and Their Regulation of Cytoplasmic Male Sterility in Wheat

Contribution of Chromosomes 1HchS and 6HchS to Fertility Restoration in the Wheat msH1 CMS System under Different Environmental Conditions

Identification of Candidate Genes and Biosynthesis Pathways Related to Fertility Conversion by Wheat KTM3315A Transcriptome Profiling

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