Development of the Taste System

Krimm, Robin F.; Thirumangalathu, Shoba; Barlow, Linda A.

doi:10.1002/9781118971758.ch33

Cited by 10 publications

(7 citation statements)

References 201 publications

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“…Gustatory gVII neurons project fibers centrally to the brainstem, specifically to the nucleus of the solitary tract (Fig. 4E, NST) (Contreras et al, 1982;Corson et al, 2012;Krimm and Barlow, 2007;Sugimoto et al, 1997). Shh-tdTomato + cells were detected in gVII at 10, 60 and 85 days (Fig.…”

Section: Sensory Neurons That Innervate Fungiform Taste Buds Express Shhmentioning

confidence: 95%

Sonic Hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance

et al. 2017

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The integrity of taste buds is intimately dependent on an intact gustatory innervation, yet the molecular nature of this dependency is unknown. Here, we show that differentiation of new taste bud cells, but not progenitor proliferation, is interrupted in mice treated with a hedgehog (Hh) pathway inhibitor (HPI), and that gustatory nerves are a source of sonic hedgehog (Shh) for taste bud renewal. Additionally, epithelial taste precursor cells express Shh transiently, and provide a local supply of Hh ligand that supports taste cell renewal. Taste buds are minimally affected when Shh is lost from either tissue source. However, when both the epithelial and neural supply of Shh are removed, taste buds largely disappear. We conclude Shh supplied by taste nerves and local taste epithelium act in concert to support continued taste bud differentiation. However, although neurally derived Shh is in part responsible for the dependence of taste cell renewal on gustatory innervation, neurotrophic support of taste buds likely involves a complex set of factors.

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Section: Sensory Neurons That Innervate Fungiform Taste Buds Express Shhmentioning

confidence: 95%

Sonic Hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance

et al. 2017

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“…Embryonic taste papillae development, especially for anterior tongue, has been well-studied [ 6 – 8 ]. Canonical developmental pathways such as Wnt, sonic hedgehog (Shh), Notch, and fibroblast growth factor (Fgf) pathways drive embryonic taste papillae development [reviewed in: 2 , 6 , 9 ]. In adult mice, taste cells survive only 3–24 days, necessitating their regeneration throughout life [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Gli3 is a negative regulator of Tas1r3-expressing taste cells

et al. 2018

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Mouse taste receptor cells survive from 3–24 days, necessitating their regeneration throughout adulthood. In anterior tongue, sonic hedgehog (SHH), released by a subpopulation of basal taste cells, regulates transcription factors Gli2 and Gli3 in stem cells to control taste cell regeneration. Using single-cell RNA-Seq we found that Gli3 is highly expressed in Tas1r3-expressing taste receptor cells and Lgr5+ taste stem cells in posterior tongue. By PCR and immunohistochemistry we found that Gli3 was expressed in taste buds in all taste fields. Conditional knockout mice lacking Gli3 in the posterior tongue (Gli3CKO) had larger taste buds containing more taste cells than did control wild-type (Gli3WT) mice. In comparison to wild-type mice, Gli3CKO mice had more Lgr5+ and Tas1r3+ cells, but fewer type III cells. Similar changes were observed ex vivo in Gli3CKO taste organoids cultured from Lgr5+ taste stem cells. Further, the expression of several taste marker and Gli3 target genes was altered in Gli3CKO mice and/or organoids. Mirroring these changes, Gli3CKO mice had increased lick responses to sweet and umami stimuli, decreased lick responses to bitter and sour taste stimuli, and increased glossopharyngeal taste nerve responses to sweet and bitter compounds. Our results indicate that Gli3 is a suppressor of stem cell proliferation that affects the number and function of mature taste cells, especially Tas1r3+ cells, in adult posterior tongue. Our findings shed light on the role of the Shh pathway in adult taste cell regeneration and may help devise strategies for treating taste distortions from chemotherapy and aging.

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“…In Neurogenin1/Neurogenin2-deficient embryos, which lack sensory nerves and Schwann cells in the limb skin, the progressive branching pattern of vessels is disrupted (Mukouyama et al, 2002;Mukouyama et al, 2005). Recently, Li et al (2013) (Kapsimali & Barlow, 2013;Krimm, Thirumangalathu, & Barlow, 2015).…”

Section: Nerves and The Vasculaturementioning

confidence: 99%

The emerging role of cranial nerves in shaping craniofacial development

Sudiwala

Knox

2019

Genesis

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Summary Organs and structures of the vertebrate head perform a plethora of tasks including visualization, digestion, vocalization/communication, auditory functions, and respiration in response to neuronal input. This input is primarily derived from afferent and efferent fibers of the cranial nerves (sensory and motor respectively) and efferent fibers of the cervical sympathetic trunk. Despite their essential contribution to the function and integration of processes necessary for survival, how organ innervation is established remains poorly understood. Furthermore, while it has been appreciated for some time that innervation of organs by cranial nerves is regulated in part by secreted factors and cell surface ligands expressed by those organs, whether nerves also regulate the development of facial organs is only beginning to be elucidated. This review will provide an overview of cranial nerve development in relation to the organs they innervate, and outline their known contributions to craniofacial development, thereby providing insight into how nerves may shape the organs they innervate during development. Throughout, the interaction between different cell and tissue types will be highlighted.

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Development of the Taste System

Cited by 10 publications

References 201 publications

Sonic Hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance

Sonic Hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance

Gli3 is a negative regulator of Tas1r3-expressing taste cells

The emerging role of cranial nerves in shaping craniofacial development

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